Zinc in PDB 6xkm: Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography

The structure of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography, PDB code: 6xkm was solved by M.Wilamowski, D.A.Sherrell, G.Minasov, Y.Kim, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Ofinfectious Diseases (Csgid), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.57 / 2.25
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	170.410, 170.410, 52.631, 90.00, 90.00, 120.00
R / Rfree (%)	20.7 / 21.3

The structure of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Zinc atom in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography (pdb code 6xkm). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography, PDB code: 6xkm:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4401 b:34.6 occ:1.00 NE2 B:HIS83 2.1 54.5 1.0 SG B:CYS74 2.3 38.5 1.0 SG B:CYS90 2.3 51.5 1.0 SG B:CYS77 2.3 39.6 1.0 CD2 B:HIS83 3.1 55.2 1.0 CE1 B:HIS83 3.1 54.8 1.0 CB B:CYS74 3.2 36.2 1.0 CB B:CYS90 3.4 56.8 1.0 CB B:CYS77 3.4 37.0 1.0 N B:CYS77 3.7 37.6 1.0 CA B:CYS77 4.0 36.7 1.0 ND1 B:HIS83 4.2 58.5 1.0 CG B:HIS83 4.2 58.4 1.0 CA B:CYS90 4.5 59.5 1.0 CA B:CYS74 4.6 35.0 1.0 CB B:TYR76 4.6 41.7 1.0 C B:TYR76 4.7 38.2 1.0 CB B:LYS93 4.8 39.5 1.0 N B:LYS93 4.9 37.8 1.0

Zinc binding site 2 out of 2 in the Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn4402 b:66.5 occ:1.00 SG B:CYS117 2.3 79.9 1.0 SG B:CYS120 2.4 80.3 1.0 SG B:CYS130 2.4 0.3 1.0 SG B:CYS128 2.4 76.5 1.0 CB B:CYS117 3.3 79.1 1.0 CB B:CYS130 3.3 0.1 1.0 CB B:CYS128 3.4 77.8 1.0 CB B:CYS120 3.4 78.2 1.0 CG1 B:VAL119 3.7 84.6 1.0 N B:CYS120 3.8 76.3 1.0 CA B:CYS128 3.9 76.3 1.0 N B:CYS130 4.0 0.2 1.0 CA B:CYS120 4.2 74.8 1.0 CA B:CYS130 4.3 0.5 1.0 N B:SER129 4.4 84.7 1.0 C B:CYS128 4.4 78.6 1.0 CA B:CYS117 4.7 75.6 1.0 C B:VAL119 4.9 77.7 1.0 N B:GLY121 5.0 63.4 1.0 C B:CYS120 5.0 68.1 1.0 CB B:VAL119 5.0 84.1 1.0

M.Wilamowski, D.A.Sherrell, G.Minasov, Y.Kim, L.Shuvalova, A.Lavens, R.Chard, M.Rosas-Lemus, N.Maltseva, R.Jedrzejczak, K.Michalska, K.J.F.Satchell, A.Joachimiak, Center For Structural Genomics Of Infectious Diseases(Csgid). Room Temperature Structure of Sars-Cov-2 NSP10/NSP16 Methyltransferase in A Complex with Sam Determined By Fixed-Target Serial Crystallography To Be Published.