Zinc in PDB 5ze2: Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C

All present enzymatic activity of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C:
2.3.2.27;

The structure of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C, PDB code: 5ze2 was solved by M.S.Kim, W.Chuenchor, X.Chen, M.Gellert, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.63 / 3.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	132.770, 108.120, 155.740, 90.00, 114.45, 90.00
R / Rfree (%)	20 / 23.7

The structure of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C also contains other interesting chemical elements:

Potassium	(K)	2 atoms
Manganese	(Mn)	4 atoms

The binding sites of Zinc atom in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C (pdb code 5ze2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C, PDB code: 5ze2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2001 b:90.6 occ:1.00 NE2 A:HIS942 2.0 88.4 1.0 NE2 A:HIS937 2.1 0.5 1.0 SG A:CYS730 2.2 86.9 1.0 SG A:CYS727 2.3 86.0 1.0 CD2 A:HIS937 2.7 91.9 1.0 CE1 A:HIS942 2.7 95.5 1.0 CB A:CYS727 2.8 86.3 1.0 CD2 A:HIS942 3.1 88.0 1.0 CB A:CYS730 3.3 94.0 1.0 CE1 A:HIS937 3.4 0.7 1.0 N A:CYS730 3.9 89.3 1.0 ND1 A:HIS942 3.9 96.5 1.0 CG A:HIS937 4.0 90.8 1.0 CG A:HIS942 4.1 89.3 1.0 CG2 A:THR732 4.1 98.5 1.0 CA A:CYS730 4.2 94.7 1.0 ND1 A:HIS937 4.3 94.2 1.0 CA A:CYS727 4.3 93.2 1.0 C A:LEU729 4.7 86.7 1.0 CB A:LEU729 4.7 85.2 1.0 CB A:HIS744 4.8 0.4 1.0 C A:CYS730 4.9 94.7 1.0 CB A:THR732 4.9 0.1 1.0 N A:CYS727 5.0 96.2 1.0

Zinc binding site 2 out of 2 in the Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Hairpin Complex, RAG1/2-Hairpin 12RSS/23RSS Complex in 5MM MN2+ For 2 Min at 4'C within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn2001 b:89.1 occ:1.00 NE2 C:HIS942 2.1 81.2 1.0 SG C:CYS727 2.2 89.4 1.0 SG C:CYS730 2.3 91.2 1.0 NE2 C:HIS937 2.3 87.5 1.0 CE1 C:HIS942 3.0 93.1 1.0 CB C:CYS730 3.0 92.2 1.0 CD2 C:HIS937 3.0 85.6 1.0 CD2 C:HIS942 3.0 84.4 1.0 CB C:CYS727 3.0 82.5 1.0 CE1 C:HIS937 3.5 96.5 1.0 N C:CYS730 3.5 93.7 1.0 CA C:CYS730 3.8 85.9 1.0 ND1 C:HIS942 4.1 89.4 1.0 CG C:HIS942 4.1 84.1 1.0 CG C:HIS937 4.3 83.4 1.0 CB C:LEU729 4.3 89.5 1.0 C C:LEU729 4.4 92.1 1.0 C C:CYS730 4.4 86.3 1.0 ND1 C:HIS937 4.5 90.0 1.0 CA C:CYS727 4.5 85.6 1.0 CG2 C:THR732 4.6 95.0 1.0 O C:CYS730 4.6 87.4 1.0 CA C:LEU729 4.7 90.6 1.0 N C:LEU729 4.8 87.2 1.0 CB C:ALA941 5.0 89.8 1.0

M.S.Kim, W.Chuenchor, X.Chen, Y.Cui, X.Zhang, Z.H.Zhou, M.Gellert, W.Yang. Cracking the Dna Code For V(D)J Recombination Mol. Cell V. 70 358 2018.
ISSN: ISSN 1097-4164
PubMed: 29628308
DOI: 10.1016/J.MOLCEL.2018.03.008