Zinc in PDB 8r70: Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4
Protein crystallography data
The structure of Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4, PDB code: 8r70
was solved by
A.Cartmell,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
113.70 /
1.80
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
53.64,
137.301,
202.842,
90,
90,
90
|
R / Rfree (%)
|
18.1 /
22.3
|
Zinc Binding Sites:
The binding sites of Zinc atom in the Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4
(pdb code 8r70). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the
Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4, PDB code: 8r70:
Jump to Zinc binding site number:
1;
2;
Zinc binding site 1 out
of 2 in 8r70
Go back to
Zinc Binding Sites List in 8r70
Zinc binding site 1 out
of 2 in the Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn703
b:30.5
occ:1.00
|
HE2
|
A:HIS490
|
1.3
|
30.0
|
0.0
|
HD1
|
A:HIS446
|
1.4
|
30.0
|
0.0
|
O
|
A:HOH876
|
2.0
|
25.8
|
1.0
|
NE2
|
A:HIS490
|
2.1
|
27.7
|
1.0
|
OD1
|
A:ASP464
|
2.2
|
23.6
|
1.0
|
O
|
A:HOH850
|
2.2
|
24.5
|
1.0
|
ND1
|
A:HIS446
|
2.2
|
30.0
|
1.0
|
O
|
A:HOH1047
|
2.3
|
24.0
|
1.0
|
CG
|
A:ASP464
|
3.0
|
27.8
|
1.0
|
CE1
|
A:HIS446
|
3.1
|
32.9
|
1.0
|
CD2
|
A:HIS490
|
3.1
|
26.5
|
1.0
|
CE1
|
A:HIS490
|
3.1
|
27.7
|
1.0
|
HE1
|
A:HIS446
|
3.1
|
30.6
|
1.0
|
HD2
|
A:HIS490
|
3.2
|
26.7
|
1.0
|
HE1
|
A:HIS490
|
3.3
|
27.9
|
1.0
|
CG
|
A:HIS446
|
3.3
|
31.9
|
1.0
|
OD2
|
A:ASP464
|
3.3
|
26.1
|
1.0
|
HB2
|
A:HIS446
|
3.6
|
32.4
|
1.0
|
HB3
|
A:HIS446
|
3.6
|
33.0
|
1.0
|
H
|
A:GLY466
|
3.7
|
24.4
|
1.0
|
H
|
A:ALA343
|
3.7
|
29.7
|
1.0
|
CB
|
A:HIS446
|
3.7
|
34.3
|
1.0
|
O
|
A:HOH930
|
3.8
|
27.8
|
1.0
|
O
|
A:ALA343
|
4.1
|
27.8
|
1.0
|
O
|
A:THR484
|
4.1
|
33.9
|
1.0
|
ND1
|
A:HIS490
|
4.1
|
28.5
|
1.0
|
CG
|
A:HIS490
|
4.2
|
27.8
|
1.0
|
NE2
|
A:HIS446
|
4.2
|
27.9
|
1.0
|
HA
|
A:PHE342
|
4.3
|
26.1
|
1.0
|
N
|
A:ALA343
|
4.3
|
31.5
|
1.0
|
HA2
|
A:GLY466
|
4.3
|
22.9
|
1.0
|
CD2
|
A:HIS446
|
4.3
|
29.0
|
1.0
|
O
|
A:ASN341
|
4.4
|
24.4
|
1.0
|
HB3
|
A:ASP448
|
4.4
|
29.8
|
1.0
|
OD2
|
A:ASP448
|
4.4
|
29.7
|
1.0
|
H
|
A:ALA465
|
4.4
|
24.2
|
1.0
|
CB
|
A:ASP464
|
4.4
|
23.9
|
1.0
|
N
|
A:GLY466
|
4.5
|
25.6
|
1.0
|
HA
|
A:ALA343
|
4.5
|
30.7
|
1.0
|
HA
|
A:ASP464
|
4.7
|
25.0
|
1.0
|
C
|
A:ALA343
|
4.7
|
29.4
|
1.0
|
N
|
A:ALA465
|
4.7
|
24.1
|
1.0
|
HB3
|
A:ASP464
|
4.8
|
24.8
|
1.0
|
CA
|
A:ALA343
|
4.8
|
30.0
|
1.0
|
C
|
A:ASP464
|
4.9
|
22.9
|
1.0
|
CA
|
A:GLY466
|
4.9
|
21.5
|
1.0
|
CA
|
A:ASP464
|
4.9
|
26.3
|
1.0
|
HD1
|
A:HIS490
|
5.0
|
30.0
|
0.0
|
|
Zinc binding site 2 out
of 2 in 8r70
Go back to
Zinc Binding Sites List in 8r70
Zinc binding site 2 out
of 2 in the Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Polysaccharide Lyase BTPL33HA (BT4410) Y291A with Ha DP4 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn702
b:35.7
occ:1.00
|
HE2
|
B:HIS490
|
1.4
|
30.0
|
0.0
|
HD1
|
B:HIS446
|
1.5
|
30.0
|
0.0
|
O
|
B:HOH809
|
1.9
|
28.9
|
1.0
|
OD1
|
B:ASP464
|
2.1
|
34.3
|
1.0
|
NE2
|
B:HIS490
|
2.1
|
28.4
|
1.0
|
O
|
B:HOH863
|
2.2
|
28.7
|
1.0
|
ND1
|
B:HIS446
|
2.3
|
36.9
|
1.0
|
O
|
B:HOH932
|
2.3
|
35.5
|
1.0
|
CD2
|
B:HIS490
|
2.9
|
28.0
|
1.0
|
HD2
|
B:HIS490
|
3.0
|
28.3
|
1.0
|
CG
|
B:ASP464
|
3.0
|
33.7
|
1.0
|
CE1
|
B:HIS446
|
3.0
|
43.5
|
1.0
|
HE1
|
B:HIS446
|
3.0
|
40.6
|
1.0
|
CE1
|
B:HIS490
|
3.3
|
30.6
|
1.0
|
OD2
|
B:ASP464
|
3.3
|
28.2
|
1.0
|
CG
|
B:HIS446
|
3.4
|
39.9
|
1.0
|
HE1
|
B:HIS490
|
3.6
|
30.0
|
1.0
|
HB3
|
B:HIS446
|
3.6
|
37.5
|
1.0
|
H
|
B:ALA343
|
3.6
|
31.7
|
1.0
|
HB2
|
B:HIS446
|
3.7
|
37.2
|
1.0
|
H
|
B:GLY466
|
3.7
|
35.1
|
1.0
|
CB
|
B:HIS446
|
3.8
|
37.3
|
1.0
|
O
|
B:HOH818
|
3.9
|
29.1
|
1.0
|
O
|
B:THR484
|
3.9
|
38.3
|
1.0
|
O
|
B:ALA343
|
4.1
|
36.2
|
1.0
|
CG
|
B:HIS490
|
4.1
|
30.0
|
1.0
|
HA
|
B:PHE342
|
4.2
|
31.9
|
1.0
|
N
|
B:ALA343
|
4.2
|
30.8
|
1.0
|
NE2
|
B:HIS446
|
4.2
|
38.6
|
1.0
|
ND1
|
B:HIS490
|
4.3
|
29.0
|
1.0
|
H
|
B:ALA465
|
4.3
|
32.7
|
1.0
|
CB
|
B:ASP464
|
4.4
|
28.6
|
1.0
|
HB3
|
B:ASP448
|
4.4
|
28.7
|
1.0
|
HA2
|
B:GLY466
|
4.4
|
35.8
|
1.0
|
CD2
|
B:HIS446
|
4.4
|
39.3
|
1.0
|
OD2
|
B:ASP448
|
4.5
|
30.7
|
1.0
|
O
|
B:ASN341
|
4.5
|
34.5
|
1.0
|
N
|
B:GLY466
|
4.5
|
36.9
|
1.0
|
HA
|
B:ALA343
|
4.6
|
35.3
|
1.0
|
HA
|
B:ASP464
|
4.6
|
29.9
|
1.0
|
HB3
|
B:ASP464
|
4.6
|
29.9
|
1.0
|
N
|
B:ALA465
|
4.7
|
33.1
|
1.0
|
C
|
B:ALA343
|
4.7
|
41.3
|
1.0
|
CA
|
B:ALA343
|
4.8
|
34.6
|
1.0
|
CA
|
B:ASP464
|
4.9
|
30.0
|
1.0
|
C
|
B:ASP464
|
4.9
|
31.6
|
1.0
|
O
|
B:HOH998
|
4.9
|
29.8
|
1.0
|
C
|
B:PHE342
|
4.9
|
30.9
|
1.0
|
CA
|
B:GLY466
|
5.0
|
34.9
|
1.0
|
|
Reference:
A.Cartmell,
M.Couturier.
Polysaccharide Lyase Family 33 Utilises An Evolutionary Conserved Mechanism of Conformationally Induced Catalysis to Drive Processivity To Be Published.
Page generated: Tue Dec 10 21:50:59 2024
|