Zinc in PDB 8fbh: Crystal Structure of NSD1 Mutant-T1927A

All present enzymatic activity of Crystal Structure of NSD1 Mutant-T1927A:
2.1.1.357;

The structure of Crystal Structure of NSD1 Mutant-T1927A, PDB code: 8fbh was solved by K.Providokhina, A.Dong, C.H.Arrowsmith, A.M.Edwards, J.Min, Structuralgenomics Consortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.17 / 2.32
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	66.293, 67.382, 70.025, 90, 90, 90
R / Rfree (%)	18.8 / 23.1

The binding sites of Zinc atom in the Crystal Structure of NSD1 Mutant-T1927A (pdb code 8fbh). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of NSD1 Mutant-T1927A, PDB code: 8fbh:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of NSD1 Mutant-T1927A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of NSD1 Mutant-T1927A within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2001 b:44.9 occ:1.00 SG A:CYS1968 2.2 40.8 1.0 SG A:CYS1921 2.3 45.5 1.0 SG A:CYS1970 2.3 44.2 1.0 SG A:CYS1975 2.4 50.5 1.0 CB A:CYS1968 3.2 41.0 1.0 CB A:CYS1975 3.3 48.9 1.0 CB A:CYS1970 3.4 43.4 1.0 CB A:CYS1921 3.5 45.2 1.0 CA A:CYS1975 3.8 54.4 1.0 N A:CYS1921 4.0 41.6 1.0 N A:CYS1970 4.0 46.6 1.0 NE2 A:HIS1919 4.1 35.0 1.0 CA A:CYS1970 4.2 46.6 1.0 O A:HOH2135 4.2 53.9 1.0 N A:SER1976 4.3 47.9 1.0 CA A:CYS1921 4.3 42.5 1.0 CD2 A:HIS1919 4.4 37.0 1.0 C A:CYS1975 4.5 56.7 1.0 CA A:CYS1968 4.5 38.8 1.0 O A:PHE1978 4.5 41.5 1.0 C A:CYS1968 4.7 46.1 1.0 N A:GLY1977 4.8 40.1 1.0 N A:GLY1971 4.9 57.3 1.0 C A:CYS1920 4.9 42.8 1.0 C A:CYS1970 4.9 48.5 1.0 OG A:SER1976 5.0 45.1 1.0 O A:CYS1968 5.0 45.8 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of NSD1 Mutant-T1927A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of NSD1 Mutant-T1927A within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2002 b:40.4 occ:1.00 SG A:CYS1793 2.3 38.6 1.0 SG A:CYS1809 2.3 40.5 1.0 SG A:CYS1803 2.3 39.4 1.0 SG A:CYS1795 2.4 39.7 1.0 CB A:CYS1795 3.1 39.0 1.0 CB A:CYS1793 3.1 42.0 1.0 CB A:CYS1803 3.2 44.2 1.0 CB A:CYS1809 3.2 37.2 1.0 CA A:CYS1803 3.5 44.3 1.0 ZN A:ZN2003 3.8 41.1 1.0 CA A:CYS1809 3.9 38.4 1.0 N A:CYS1803 4.0 40.3 1.0 N A:CYS1795 4.0 40.1 1.0 CA A:CYS1795 4.1 42.8 1.0 SG A:CYS1818 4.4 41.9 1.0 CA A:CYS1793 4.5 42.0 1.0 SG A:CYS1823 4.7 41.9 1.0 N A:ASN1794 4.8 45.4 1.0 C A:CYS1809 4.8 39.7 1.0 C A:CYS1793 4.9 47.0 1.0 OD1 A:ASN1794 4.9 54.9 1.0 C A:CYS1803 4.9 42.5 1.0 O A:HOH2150 5.0 32.9 1.0 C A:PRO1802 5.0 45.6 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of NSD1 Mutant-T1927A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of NSD1 Mutant-T1927A within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2003 b:41.1 occ:1.00 SG A:CYS1803 2.3 39.4 1.0 SG A:CYS1818 2.3 41.9 1.0 SG A:CYS1823 2.3 41.9 1.0 SG A:CYS1829 2.4 45.5 1.0 CB A:CYS1818 3.1 45.4 1.0 CB A:CYS1803 3.3 44.2 1.0 CB A:CYS1823 3.4 41.7 1.0 CB A:CYS1829 3.6 40.4 1.0 ZN A:ZN2002 3.8 40.4 1.0 O A:HOH2150 3.9 32.9 1.0 SG A:CYS1793 4.1 38.6 1.0 CA A:CYS1829 4.2 46.8 1.0 CB A:ASN1831 4.3 39.3 1.0 CA A:CYS1818 4.6 44.1 1.0 CA A:CYS1823 4.7 45.2 1.0 CA A:CYS1803 4.7 44.3 1.0 CB A:ALA1825 4.8 46.5 1.0 SG A:CYS1809 4.9 40.5 1.0

K.Providokhina, A.Dong, C.H.Arrowsmith, A.M.Edwards, J.Min, Structural Genomics Consortium (Sgc). Crystal Structure of NSD1 To Be Published.