Zinc in PDB 9lhu: Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn

All present enzymatic activity of Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn:
5.1.2.7;

The structure of Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn, PDB code: 9lhu was solved by H.L.Wei, Y.X.Wang, W.D.Liu, L.L.Zhu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.88 / 2.43
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	66.784, 85.533, 108.516, 90, 90, 90
R / Rfree (%)	21.8 / 25.9

The binding sites of Zinc atom in the Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn (pdb code 9lhu). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn, PDB code: 9lhu:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:147.3 occ:1.00 O A:HOH762 1.9 60.5 1.0 ND1 A:HIS355 2.4 73.2 1.0 NE2 A:HIS175 2.7 73.0 1.0 OD2 A:ASP284 3.3 80.7 1.0 CD2 A:HIS175 3.3 69.6 1.0 CG A:HIS355 3.4 70.7 1.0 CE1 A:HIS355 3.5 68.2 1.0 CB A:HIS355 3.5 60.0 1.0 CE1 A:HIS175 3.9 82.4 1.0 OD2 A:ASP174 4.2 57.8 1.0 CG A:ASP284 4.4 76.5 1.0 CD2 A:HIS355 4.5 56.0 1.0 CA A:HIS355 4.5 55.5 1.0 N A:SER356 4.5 62.9 1.0 NE2 A:HIS355 4.6 56.9 1.0 CG A:HIS175 4.6 62.1 1.0 ND1 A:HIS175 4.8 63.4 1.0 OG A:SER356 4.9 71.2 1.0 OD1 A:ASP174 4.9 51.4 1.0 CG A:ASP174 5.0 57.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of An Agose Isomerase Mutant 5 (TST4EASE M5) From Thermotogota Bacterium with Zn within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn602 b:75.3 occ:1.00 NE2 A:HIS200 2.3 66.5 1.0 ND1 A:HIS261 2.3 58.1 1.0 CE1 A:HIS200 2.9 61.8 1.0 CE1 A:HIS261 3.1 59.3 1.0 CD2 A:HIS200 3.4 56.8 1.0 CG A:HIS261 3.4 51.8 1.0 CB A:HIS261 3.8 53.4 1.0 ND1 A:HIS200 4.1 64.8 1.0 CG A:HIS200 4.3 58.1 1.0 NE2 A:HIS261 4.3 48.9 1.0 CA A:HIS261 4.3 40.3 1.0 CE A:LYS264 4.4 75.4 1.0 CD2 A:HIS261 4.4 46.8 1.0 NZ A:LYS264 4.8 73.3 1.0

Y.Wang, Z.Tan, H.Wei, N.Zhang, L.Wang, Y.Yang, W.Liu, L.Zhu. Protein Engineering of Tagatose 4-Epimerase For D-Tagatose Production. J.Agric.Food Chem. V. 73 12353 2025.
ISSN: ESSN 1520-5118
PubMed: 40350604
DOI: 10.1021/ACS.JAFC.5C01663