Zinc in PDB 8eos: M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp

All present enzymatic activity of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp:
2.7.7.6;

The structure of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp (pdb code 8eos). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp, PDB code: 8eos:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2003 b:117.3 occ:1.00 SG D:CYS978 2.3 57.8 1.0 SG D:CYS975 2.3 45.9 1.0 SG D:CYS968 2.3 44.7 1.0 SG D:CYS891 2.3 57.0 1.0 CB D:CYS978 3.3 40.7 1.0 CB D:CYS891 3.5 45.2 1.0 CB D:CYS968 3.6 36.8 1.0 CB D:CYS975 3.7 32.2 1.0 CA D:CYS968 4.0 41.0 1.0 NH2 D:ARG963 4.1 41.3 1.0 N D:CYS891 4.2 46.1 1.0 N D:CYS978 4.3 47.7 1.0 N D:ALA969 4.3 42.0 1.0 CA D:CYS978 4.4 37.8 1.0 N D:CYS975 4.4 29.4 1.0 CA D:CYS891 4.5 45.3 1.0 CA D:CYS975 4.6 31.9 1.0 CG2 D:THR970 4.6 43.6 1.0 C D:CYS968 4.8 45.3 1.0 CG2 D:THR893 4.8 56.6 1.0 O D:CYS975 5.0 42.0 1.0

Zinc binding site 2 out of 2 in the M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of M. Tuberculosis Rnap Elongation Complex with Nusg and Cmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2004 b:194.6 occ:1.00 SG D:CYS60 2.3 121.6 1.0 SG D:CYS62 2.3 128.2 1.0 SG D:CYS78 2.3 132.1 1.0 SG D:CYS75 2.3 127.6 1.0 CB D:CYS78 3.2 128.2 1.0 N D:CYS62 3.4 126.8 1.0 CB D:CYS62 3.4 126.8 1.0 CB D:CYS75 3.5 126.3 1.0 N D:CYS78 3.6 129.9 1.0 CA D:CYS62 4.0 125.5 1.0 CB D:CYS60 4.0 122.7 1.0 CA D:CYS78 4.0 127.9 1.0 N D:TYR61 4.1 120.3 1.0 CB D:ARG77 4.3 142.7 1.0 C D:TYR61 4.4 121.9 1.0 C D:ARG77 4.5 142.4 1.0 CA D:TYR61 4.6 120.6 1.0 N D:GLY63 4.6 131.8 1.0 CB D:TYR61 4.7 120.1 1.0 CA D:ARG77 4.8 142.2 1.0 C D:CYS62 4.9 126.2 1.0 NH1 D:ARG77 4.9 144.2 1.0 N D:ARG77 4.9 140.9 1.0 CG D:LYS64 4.9 142.9 1.0 CA D:CYS75 4.9 127.8 1.0 CB D:LYS64 5.0 143.5 1.0 C D:CYS60 5.0 122.0 1.0

R.K.Vishwakarma, M.Z.Qayyum, P.Babitzke, K.S.Murakami. Structural Basis of Nusg-Dependent Rna Polymerase Transcription Pausing in Bacteria To Be Published.