Zinc in PDB 8eha: Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out)

All present enzymatic activity of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out):
2.7.7.6;

The structure of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out) (pdb code 8eha). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out), PDB code: 8eha:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out) within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1501 b:147.4 occ:1.00 SG J:CYS85 2.3 92.8 1.0 SG J:CYS70 2.3 86.0 1.0 SG J:CYS88 2.3 90.7 1.0 SG J:CYS72 2.3 93.4 1.0 CB J:CYS72 2.8 93.4 1.0 CB J:CYS85 3.1 92.8 1.0 N J:CYS72 3.4 93.4 1.0 CA J:CYS72 3.6 93.4 1.0 N J:CYS88 3.9 90.7 1.0 CB J:CYS70 3.9 86.0 1.0 CB J:CYS88 3.9 90.7 1.0 N J:GLY73 4.3 87.8 1.0 CG1 J:VAL90 4.4 76.0 1.0 CA J:CYS88 4.4 90.7 1.0 C J:CYS72 4.4 93.4 1.0 N J:LEU71 4.5 83.8 1.0 CA J:CYS85 4.5 92.8 1.0 N J:GLY89 4.5 83.0 1.0 C J:LEU71 4.6 83.8 1.0 CB J:LYS87 4.6 92.3 1.0 N J:LYS87 4.7 92.3 1.0 C J:CYS70 4.8 86.0 1.0 C J:CYS88 4.8 90.7 1.0 N J:LYS74 4.8 85.6 1.0 C J:LYS87 4.9 92.3 1.0 CB J:LYS74 4.9 85.6 1.0 CA J:CYS70 4.9 86.0 1.0 CA J:LEU71 5.0 83.8 1.0 CA J:LYS87 5.0 92.3 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (Out) within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:70.7 occ:1.00 SG J:CYS898 2.3 42.9 1.0 SG J:CYS895 2.3 43.7 1.0 SG J:CYS888 2.3 45.2 1.0 SG J:CYS814 2.3 51.3 1.0 CB J:CYS898 2.6 42.9 1.0 CB J:CYS814 3.1 51.3 1.0 NH2 J:ARG883 3.3 47.5 1.0 CB J:CYS888 3.4 45.2 1.0 CB J:CYS895 3.6 43.7 1.0 CA J:CYS888 3.8 45.2 1.0 CA J:CYS898 3.9 42.9 1.0 N J:ASP889 4.0 52.4 1.0 N J:CYS814 4.1 51.3 1.0 N J:CYS898 4.2 42.9 1.0 CA J:CYS814 4.2 51.3 1.0 N J:CYS895 4.3 43.7 1.0 CZ J:ARG883 4.5 47.5 1.0 CA J:CYS895 4.5 43.7 1.0 C J:CYS888 4.5 45.2 1.0 OG1 J:THR816 4.7 52.0 1.0 NE J:ARG883 4.7 47.5 1.0 O J:CYS895 4.8 43.7 1.0 C J:CYS895 5.0 43.7 1.0

J.Y.Kang, T.V.Mishanina, Y.Bao, J.Chen, E.Llewellyn, J.Liu, S.A.Darst, R.Landick. An Ensemble of Interconverting Conformations of the Elemental Paused Transcription Complex Creates Regulatory Options. Proc.Natl.Acad.Sci.Usa V. 120 45120 2023.
ISSN: ESSN 1091-6490
PubMed: 36795753
DOI: 10.1073/PNAS.2215945120