Zinc in PDB 7mfd: Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved

All present enzymatic activity of Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved:
2.7.11.1; 2.7.12.2;

The structure of Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved also contains other interesting chemical elements:

Fluorine

(F)

1 atom

The binding sites of Zinc atom in the Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved (pdb code 7mfd). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved, PDB code: 7mfd:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn901 b:84.8 occ:1.00 SG A:CYS248 2.3 80.3 1.0 SG A:CYS251 2.3 66.3 1.0 SG A:CYS272 2.3 90.1 1.0 CB A:CYS248 3.4 80.3 1.0 CB A:CYS272 3.5 90.1 1.0 N A:CYS251 3.6 66.3 1.0 CB A:CYS251 3.6 66.3 1.0 CB A:HIS269 3.8 92.2 1.0 N A:HIS269 4.0 92.2 1.0 CA A:CYS251 4.1 66.3 1.0 CB A:PHE250 4.5 71.1 1.0 CA A:HIS269 4.5 92.2 1.0 C A:PHE250 4.5 71.1 1.0 N A:PHE250 4.5 71.1 1.0 C A:CYS251 4.7 66.3 1.0 O A:HIS269 4.7 92.2 1.0 N A:ARG252 4.7 59.4 1.0 CA A:PHE250 4.7 71.1 1.0 CA A:CYS272 4.8 90.1 1.0 CA A:CYS248 4.8 80.3 1.0 CB A:LYS253 4.9 59.0 1.0 N A:LYS253 5.0 59.0 1.0

Zinc binding site 2 out of 2 in the Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Autoinhibited Braf:(14-3-3)2:Mek Complex with the Braf Rbd Resolved within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn902 b:60.8 occ:1.00 SG A:CYS264 2.5 69.9 1.0 CB A:CYS264 2.8 69.9 1.0 SG A:CYS261 2.8 69.9 1.0 CD2 A:HIS235 3.1 51.2 1.0 NE2 A:HIS235 3.9 51.2 1.0 N A:CYS264 3.9 69.9 1.0 CB A:CYS261 4.0 69.9 1.0 CA A:CYS264 4.0 69.9 1.0 NZ A:LYS687 4.1 92.4 1.0 CG2 A:THR263 4.1 65.0 1.0 CA A:HIS235 4.1 51.2 1.0 N A:HIS235 4.2 51.2 1.0 CG A:HIS235 4.2 51.2 1.0 CB A:HIS235 4.8 51.2 1.0 C A:CYS264 5.0 69.9 1.0

J.A.Martinez Fiesco, D.E.Durrant, D.K.Morrison, P.Zhang. Structural Insights Into the Braf Monomer-to-Dimer Transition Mediated By Ras Binding. Nat Commun V. 13 486 2022.
ISSN: ESSN 2041-1723
PubMed: 35078985
DOI: 10.1038/S41467-022-28084-3