Zinc in PDB 7knj: C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine

All present enzymatic activity of C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine:
2.7.11.13;

The structure of C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine, PDB code: 7knj was solved by S.S.Katti, I.Krieger, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.24 / 1.57
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	34.205, 26.138, 58.158, 90, 96.92, 90
R / Rfree (%)	17.8 / 22.5

The binding sites of Zinc atom in the C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine (pdb code 7knj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine, PDB code: 7knj:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn303 b:12.9 occ:1.00 ND1 A:HIS231 2.0 11.4 1.0 SG A:CYS264 2.3 13.2 1.0 SG A:CYS280 2.3 13.6 1.0 SG A:CYS261 2.3 9.7 1.0 CE1 A:HIS231 2.9 16.8 1.0 CG A:HIS231 3.1 10.7 1.0 CB A:CYS264 3.2 13.0 1.0 CB A:CYS280 3.3 17.7 1.0 CB A:CYS261 3.3 7.2 1.0 CB A:HIS231 3.6 11.7 1.0 N A:CYS264 3.7 12.7 1.0 CA A:HIS231 3.8 10.7 1.0 CA A:CYS280 3.9 18.7 1.0 NE2 A:HIS231 4.0 15.1 1.0 CA A:CYS264 4.0 13.2 1.0 CD2 A:HIS231 4.2 15.6 1.0 O A:HOH436 4.4 28.4 1.0 CA A:CYS261 4.7 9.9 1.0 C A:ASP263 4.7 16.5 1.0 N A:ARG232 4.7 7.8 1.0 CB A:ASP263 4.7 16.3 1.0 C A:CYS280 4.7 30.1 1.0 N A:HIS231 4.8 12.8 1.0 C A:CYS264 4.8 13.5 1.0 C A:HIS231 4.9 12.2 1.0 CB A:MET266 4.9 13.1 0.6 O A:HOH434 4.9 22.0 1.0 N A:GLY265 4.9 8.9 1.0 CB A:MET266 4.9 13.1 0.4 N A:ASP263 5.0 9.7 1.0

Zinc binding site 2 out of 2 in the C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of C1B Domain of Protein Kinase C in Complex with Phorbol Ester and Phosphatidylcholine within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn304 b:11.3 occ:1.00 ND1 A:HIS269 2.1 11.4 1.0 SG A:CYS247 2.3 11.8 1.0 SG A:CYS272 2.3 13.4 1.0 SG A:CYS244 2.3 8.5 1.0 CE1 A:HIS269 3.0 10.6 1.0 CG A:HIS269 3.1 10.9 1.0 CB A:CYS244 3.1 9.2 1.0 CB A:CYS247 3.3 15.3 1.0 CB A:CYS272 3.4 13.1 1.0 CB A:HIS269 3.4 9.3 1.0 N A:CYS247 3.8 13.3 1.0 N A:HIS269 3.9 8.2 1.0 CA A:CYS247 4.1 10.0 1.0 NE2 A:HIS269 4.2 10.0 1.0 O A:HOH437 4.2 28.5 1.0 CD2 A:HIS269 4.2 11.1 1.0 CA A:HIS269 4.3 8.7 1.0 CA A:CYS244 4.6 6.6 1.0 CB A:HIS246 4.6 11.9 1.0 CA A:CYS272 4.7 11.2 1.0 C A:CYS247 4.8 9.2 1.0 OG A:SER249 4.9 19.4 1.0 C A:HIS246 4.9 13.3 1.0 CD2 A:HIS246 5.0 13.5 1.0

S.S.Katti, I.V.Krieger, J.Ann, J.Lee, J.C.Sacchettini, T.I.Igumenova. Structural Anatomy of Protein Kinase C C1 Domain Interactions with Diacylglycerol and Other Agonists. Nat Commun V. 13 2695 2022.
ISSN: ESSN 2041-1723
PubMed: 35577811
DOI: 10.1038/S41467-022-30389-2