Zinc in PDB 7b4a: Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna

The structure of Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna, PDB code: 7b4a was solved by D.Golovenko, H.Rozenberg, O.Degtjarik, Z.Shakked, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.07 / 1.90
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	136.784, 50.347, 67.481, 90, 92.75, 90
R / Rfree (%)	20.7 / 25.4

The binding sites of Zinc atom in the Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna (pdb code 7b4a). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna, PDB code: 7b4a:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:12.5 occ:1.00 ND1 A:HIS179 2.1 18.4 1.0 SG A:CYS176 2.3 16.3 1.0 SG A:CYS238 2.3 12.3 1.0 SG A:CYS242 2.3 13.5 1.0 CE1 A:HIS179 2.9 18.4 1.0 CB A:CYS242 3.1 12.7 1.0 CG A:HIS179 3.1 18.5 1.0 CB A:CYS238 3.1 12.2 1.0 CB A:CYS176 3.4 16.5 1.0 CB A:HIS179 3.5 18.6 1.0 CA A:CYS238 3.7 12.1 1.0 NE2 A:HIS179 4.1 22.2 1.0 N A:CYS176 4.1 15.8 1.0 CD2 A:HIS179 4.2 21.8 1.0 CA A:CYS176 4.3 16.3 1.0 O A:MET237 4.3 14.2 1.0 N A:HIS179 4.3 18.9 1.0 N A:ASN239 4.4 12.0 1.0 CA A:CYS242 4.5 12.4 1.0 CA A:HIS179 4.6 19.7 1.0 C A:CYS238 4.6 12.0 1.0 O A:HOH484 4.7 4.6 1.0 N A:CYS238 4.7 12.1 1.0 O A:CYS176 4.9 17.6 1.0 C A:CYS176 4.9 16.4 1.0 C A:MET237 5.0 17.8 1.0

Zinc binding site 2 out of 2 in the Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structural Basis of Reactivation of Oncogenic P53 Mutants By A Small Molecule: Methylene Quinuclidinone (Mq). Human P53DBD-R273H Mutant Bound to Dna: R273H-Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn301 b:10.4 occ:1.00 ND1 B:HIS179 2.0 7.5 1.0 SG B:CYS238 2.3 15.9 1.0 SG B:CYS242 2.3 15.1 1.0 SG B:CYS176 2.3 12.1 1.0 CE1 B:HIS179 2.9 7.4 1.0 CG B:HIS179 3.1 12.2 1.0 CB B:CYS242 3.2 14.9 1.0 CB B:CYS238 3.2 13.8 1.0 CB B:CYS176 3.3 11.3 1.0 CB B:HIS179 3.5 12.9 1.0 CA B:CYS238 3.8 17.6 1.0 NE2 B:HIS179 4.1 17.7 1.0 N B:CYS176 4.1 13.6 1.0 CD2 B:HIS179 4.2 13.0 1.0 CA B:CYS176 4.3 21.6 1.0 N B:HIS179 4.4 15.0 1.0 O B:MET237 4.4 15.1 1.0 CA B:CYS242 4.6 15.4 1.0 CA B:HIS179 4.6 12.8 1.0 N B:ASN239 4.6 14.8 1.0 O B:HOH487 4.6 18.0 1.0 C B:CYS238 4.7 14.4 1.0 N B:CYS238 4.8 13.6 1.0 O B:CYS176 4.8 15.1 1.0 C B:CYS176 4.9 12.7 1.0

O.Degtjarik, D.Golovenko, Y.Diskin-Posner, L.Abrahmsen, H.Rozenberg, Z.Shakked. P53 Structure 4 To Be Published.