Zinc in PDB 7ay2: Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg

All present enzymatic activity of Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg:
3.4.19.12;

The structure of Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg, PDB code: 7ay2 was solved by C.Arkinson, M.L.Rennie, H.Walden, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	67.12 / 3.20
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	134.242, 134.242, 274.673, 90, 90, 120
R / Rfree (%)	20.4 / 23.4

The binding sites of Zinc atom in the Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg (pdb code 7ay2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg, PDB code: 7ay2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1000 b:70.6 occ:1.00 SG B:CYS440 2.1 59.1 1.0 SG B:CYS506 2.1 51.8 1.0 SG B:CYS509 2.2 53.6 1.0 SG B:CYS443 2.2 54.6 1.0 CB B:CYS440 3.0 58.9 1.0 CB B:CYS506 3.1 55.1 1.0 CB B:CYS443 3.4 58.8 1.0 CB B:CYS509 3.5 53.4 1.0 N B:CYS443 3.5 63.0 1.0 CA B:CYS443 4.0 57.5 1.0 N B:CYS509 4.0 59.1 1.0 CB B:GLU442 4.1 58.1 1.0 ND2 B:ASN508 4.4 62.8 1.0 CA B:CYS509 4.4 54.8 1.0 CA B:CYS440 4.4 59.5 1.0 C B:GLU442 4.5 60.8 1.0 CG2 B:THR513 4.5 57.6 1.0 OG1 B:THR513 4.6 54.6 1.0 CA B:CYS506 4.6 57.3 1.0 CA B:GLU442 4.7 58.6 1.0 N B:GLU442 4.7 57.5 1.0 C B:CYS440 4.8 59.9 1.0 O B:CYS440 4.8 59.8 1.0 CB B:ASN508 4.8 56.5 1.0 C B:CYS443 4.8 58.1 1.0 N B:GLU444 4.9 63.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Truncated USP1-UAF1 Reacted with Ubiquitin-Prg within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn1000 b:82.3 occ:1.00 SG E:CYS506 2.2 65.8 1.0 SG E:CYS509 2.2 65.0 1.0 SG E:CYS443 2.3 69.9 1.0 SG E:CYS440 2.3 80.0 1.0 CB E:CYS506 3.1 72.5 1.0 CB E:CYS440 3.3 62.1 1.0 CB E:CYS443 3.3 72.6 1.0 CB E:CYS509 3.4 66.8 1.0 N E:CYS443 3.6 70.3 1.0 N E:CYS509 3.9 70.6 1.0 CA E:CYS443 4.1 68.1 1.0 CA E:CYS509 4.2 67.6 1.0 ND2 E:ASN508 4.2 67.5 1.0 CB E:GLU442 4.3 71.6 1.0 CA E:CYS506 4.6 81.2 1.0 OG1 E:THR513 4.6 82.8 1.0 CB E:ASN508 4.6 63.6 1.0 CG2 E:THR513 4.7 75.0 1.0 C E:GLU442 4.7 70.5 1.0 CA E:CYS440 4.7 76.5 1.0 C E:ASN508 4.9 71.9 1.0 C E:CYS443 4.9 73.0 1.0 CA E:GLU442 4.9 72.5 1.0 N E:GLU442 4.9 72.6 1.0 CG E:ASN508 4.9 72.5 1.0

M.L.Rennie, C.Arkinson, V.K.Chaugule, R.Toth, H.Walden. Structural Basis of FANCD2 Deubiquitination By USP1-UAF1 Biorxiv 2020.
DOI: 10.1101/2020.12.05.412924