Zinc in PDB 6o7h: Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System

The binding sites of Zinc atom in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System (pdb code 6o7h). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System, PDB code: 6o7h:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn801 b:64.0 occ:1.00 SG A:CYS389 2.4 26.1 1.0 SG A:CYS392 2.4 20.4 1.0 SG A:CYS416 2.4 30.7 1.0 SG A:CYS413 2.5 30.2 1.0 CB A:CYS389 3.1 26.1 1.0 CB A:CYS392 3.5 20.4 1.0 N A:CYS392 3.6 20.4 1.0 CB A:CYS416 3.7 30.7 1.0 CB A:CYS413 3.9 30.2 1.0 CA A:CYS392 4.0 20.4 1.0 N A:CYS413 4.3 30.2 1.0 CB A:VAL391 4.3 19.7 1.0 N A:GLY393 4.4 20.4 1.0 CA A:CYS389 4.5 26.1 1.0 OH A:TYR258 4.6 21.1 1.0 C A:CYS392 4.6 20.4 1.0 C A:VAL391 4.6 19.7 1.0 CA A:CYS413 4.6 30.2 1.0 N A:VAL391 4.7 19.7 1.0 CA A:VAL391 4.8 19.7 1.0 CA A:CYS416 4.8 30.7 1.0 NH1 A:ARG394 4.8 23.6 1.0 N A:CYS416 4.8 30.7 1.0 O A:CYS413 4.9 30.2 1.0 N A:ARG394 5.0 23.6 1.0

Zinc binding site 2 out of 3 in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:43.4 occ:1.00 NE2 C:HIS111 2.2 21.3 1.0 SG C:CYS122 2.4 18.2 1.0 SG C:CYS125 2.4 17.2 1.0 SG C:CYS113 2.5 24.9 1.0 CD2 C:HIS111 2.9 21.3 1.0 CB C:CYS125 3.0 17.2 1.0 CE1 C:HIS111 3.2 21.3 1.0 CB C:CYS122 3.4 18.2 1.0 CB C:CYS113 3.5 24.9 1.0 N C:CYS125 3.5 17.2 1.0 N C:CYS113 3.6 24.9 1.0 CA C:CYS125 3.9 17.2 1.0 CG C:HIS111 4.0 21.3 1.0 ND1 C:HIS111 4.1 21.3 1.0 CA C:CYS113 4.1 24.9 1.0 CB C:VAL124 4.4 21.0 1.0 C C:VAL112 4.6 21.9 1.0 C C:VAL124 4.6 21.0 1.0 CA C:VAL112 4.8 21.9 1.0 CA C:CYS122 4.8 18.2 1.0 O C:HIS111 4.8 21.3 1.0 CA C:VAL124 4.9 21.0 1.0 CG1 C:VAL124 4.9 21.0 1.0 N C:VAL124 4.9 21.0 1.0

Zinc binding site 3 out of 3 in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Complex with CA4 in Type III-A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn301 b:47.6 occ:1.00 NE2 D:HIS111 2.2 29.9 1.0 SG D:CYS122 2.5 31.1 1.0 SG D:CYS113 2.5 32.5 1.0 SG D:CYS125 2.5 23.1 1.0 CD2 D:HIS111 2.8 29.9 1.0 CB D:CYS125 3.1 23.1 1.0 CE1 D:HIS111 3.3 29.9 1.0 CB D:CYS122 3.4 31.1 1.0 N D:CYS125 3.4 23.1 1.0 CB D:CYS113 3.8 32.5 1.0 CA D:CYS125 3.9 23.1 1.0 CB D:VAL124 3.9 25.6 1.0 N D:CYS113 4.0 32.5 1.0 CG D:HIS111 4.0 29.9 1.0 ND1 D:HIS111 4.2 29.9 1.0 C D:VAL124 4.4 25.6 1.0 CA D:CYS113 4.4 32.5 1.0 CG1 D:VAL124 4.4 25.6 1.0 CA D:VAL124 4.5 25.6 1.0 N D:VAL124 4.6 25.6 1.0 O D:HIS111 4.8 29.9 1.0 CA D:CYS122 4.8 31.1 1.0 CG2 D:VAL124 4.8 25.6 1.0 C D:VAL112 4.9 28.6 1.0 CA D:VAL112 5.0 28.6 1.0

N.Jia, R.Jones, G.Sukenick, D.J.Patel. Second Messenger CA4FORMATION Within the Composite CSM1 Palm Pocket of Type III-A Crispr-Cas Csm Complex and Its Release Path. Mol.Cell V. 75 933 2019.
ISSN: ISSN 1097-2765
PubMed: 31326272
DOI: 10.1016/J.MOLCEL.2019.06.013