Zinc in PDB 5zx2: Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna

All present enzymatic activity of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna:
2.7.7.6;

The structure of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna, PDB code: 5zx2 was solved by L.Li, Y.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.94 / 2.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	129.824, 164.027, 214.792, 90.00, 90.00, 90.00
R / Rfree (%)	22 / 25.5

The binding sites of Zinc atom in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna (pdb code 5zx2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna, PDB code: 5zx2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2001 b:72.5 occ:1.00 SG D:CYS62 2.1 69.7 1.0 SG D:CYS75 2.1 66.1 1.0 SG D:CYS78 2.1 65.8 1.0 SG D:CYS60 2.3 61.4 1.0 CB D:CYS75 2.8 59.2 1.0 CB D:CYS60 3.3 55.7 1.0 CB D:CYS62 3.4 58.8 1.0 CB D:CYS78 3.4 62.8 1.0 N D:CYS62 3.6 68.1 1.0 N D:CYS78 3.8 61.5 1.0 CA D:CYS62 4.0 66.7 1.0 CA D:CYS78 4.2 57.7 1.0 N D:TYR61 4.3 58.1 1.0 N D:GLY63 4.3 62.1 1.0 CA D:CYS75 4.3 61.8 1.0 N D:LYS64 4.3 63.7 1.0 CB D:LYS64 4.3 66.0 1.0 C D:CYS60 4.5 58.1 1.0 CA D:CYS60 4.5 55.0 1.0 C D:CYS62 4.5 64.7 1.0 CD2 D:TYR65 4.6 68.8 1.0 CB D:ARG77 4.7 69.5 1.0 C D:TYR61 4.7 60.9 1.0 C D:CYS75 4.9 65.1 1.0 C D:ARG77 4.9 64.0 1.0 CA D:LYS64 4.9 63.6 1.0 N D:TYR65 5.0 59.3 1.0

Zinc binding site 2 out of 2 in the Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mycobacterium Tuberculosis Rna Polymerase Transcription Initiation Complex with Ecf Sigma Factor Sigma H and 7NT Rna within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2002 b:66.0 occ:1.00 SG D:CYS978 2.1 57.9 1.0 SG D:CYS975 2.1 51.7 1.0 SG D:CYS968 2.2 58.5 1.0 SG D:CYS891 2.2 68.9 1.0 CB D:CYS978 2.8 57.2 1.0 CB D:CYS968 3.2 59.3 1.0 CB D:CYS975 3.4 57.6 1.0 CA D:CYS968 3.5 60.6 1.0 CB D:CYS891 3.6 62.8 1.0 N D:ALA969 4.0 63.4 1.0 N D:CYS975 4.0 54.1 1.0 CA D:CYS978 4.0 54.9 1.0 N D:CYS978 4.1 55.2 1.0 OG1 D:THR970 4.1 60.4 1.0 CA D:CYS975 4.2 52.9 1.0 NH1 D:ARG963 4.2 62.3 1.0 C D:CYS968 4.2 62.1 1.0 N D:CYS891 4.3 64.6 1.0 O D:CYS975 4.4 49.1 1.0 CG2 D:THR893 4.4 70.1 1.0 CA D:CYS891 4.5 66.1 1.0 C D:CYS975 4.6 50.7 1.0 N D:THR970 4.7 62.7 1.0 N D:CYS968 4.8 60.5 1.0

L.Li, C.Fang, N.Zhuang, T.Wang, Y.Zhang. Structural Basis For Transcription Initiation By Bacterial Ecf Sigma Factors. Nat Commun V. 10 1153 2019.
ISSN: ESSN 2041-1723
PubMed: 30858373
DOI: 10.1038/S41467-019-09096-Y