Zinc in PDB 5yf4: A Kinase Complex MST4-MOB4

All present enzymatic activity of A Kinase Complex MST4-MOB4:
2.7.11.1;

The structure of A Kinase Complex MST4-MOB4, PDB code: 5yf4 was solved by M.Chen, Z.C.Zhou, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.04 / 1.90
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	88.469, 33.997, 61.023, 90.00, 122.67, 90.00
R / Rfree (%)	15.3 / 19.8

The binding sites of Zinc atom in the A Kinase Complex MST4-MOB4 (pdb code 5yf4). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the A Kinase Complex MST4-MOB4, PDB code: 5yf4:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the A Kinase Complex MST4-MOB4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of A Kinase Complex MST4-MOB4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:14.5 occ:1.00 ND1 A:HIS127 2.1 14.8 1.0 NE2 A:HIS113 2.1 15.7 1.0 SG A:CYS119 2.3 14.4 1.0 SG A:CYS110 2.3 13.8 1.0 CD2 A:HIS113 2.9 14.1 1.0 CE1 A:HIS127 3.0 15.9 1.0 CB A:CYS110 3.0 17.5 1.0 CG A:HIS127 3.1 13.8 1.0 CE1 A:HIS113 3.2 15.8 1.0 CB A:HIS127 3.5 13.9 1.0 CB A:CYS119 3.6 12.6 1.0 NE2 A:HIS127 4.1 14.8 1.0 CG A:HIS113 4.1 15.7 1.0 ND1 A:HIS113 4.2 17.3 1.0 CD2 A:HIS127 4.2 15.5 1.0 O A:ASP123 4.3 13.1 1.0 CA A:CYS110 4.4 13.6 1.0 CB A:ALA112 4.7 22.3 1.0 C A:ASP123 4.7 13.9 1.0 O A:HOH402 4.7 31.1 1.0 CA A:TYR124 4.7 10.8 1.0 CD A:LYS117 4.8 25.8 1.0 N A:TYR124 4.8 9.0 1.0 C A:CYS110 4.8 15.7 1.0 CB A:ASP123 4.9 10.9 1.0 CA A:CYS119 4.9 11.3 1.0 O A:CYS110 4.9 17.1 1.0 N A:ALA112 5.0 17.7 1.0

Zinc binding site 2 out of 2 in the A Kinase Complex MST4-MOB4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of A Kinase Complex MST4-MOB4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn302 b:11.7 occ:1.00 NE2 A:HIS174 2.0 15.4 1.0 NE2 A:HIS169 2.1 8.9 1.0 SG A:CYS92 2.3 12.3 1.0 SG A:CYS97 2.3 14.5 1.0 CE1 A:HIS174 3.0 14.7 1.0 CE1 A:HIS169 3.0 10.2 1.0 CD2 A:HIS174 3.1 12.5 1.0 CD2 A:HIS169 3.1 10.5 1.0 CB A:CYS97 3.1 13.8 1.0 CB A:CYS92 3.2 12.2 1.0 CA A:CYS92 3.7 14.9 1.0 CB A:ALA121 3.9 10.1 1.0 ND1 A:HIS174 4.1 16.7 1.0 ND1 A:HIS169 4.2 9.7 1.0 CG A:HIS174 4.2 15.4 1.0 O A:HOH414 4.2 29.3 1.0 CG A:HIS169 4.2 9.6 1.0 CA A:CYS97 4.6 11.6 1.0 C A:CYS92 4.6 21.2 1.0 CB A:HIS173 4.7 9.1 1.0 O A:HOH459 4.8 23.8 1.0 O A:HOH445 4.9 12.7 1.0 N A:CYS92 4.9 15.1 1.0

M.Chen, H.Zhang, Z.Shi, Y.Li, X.Zhang, Z.Gao, L.Zhou, J.Ma, Q.Xu, J.Guan, Y.Cheng, S.Jiao, Z.C.Zhou. The MST4-MOB4 Complex Disrupts the MST1-MOB1 Complex in the Hippo-Yap Pathway and Plays A Pro-Oncogenic Role in Pancreatic Cancer. J. Biol. Chem. V. 293 14455 2018.
ISSN: ESSN 1083-351X
PubMed: 30072378
DOI: 10.1074/JBC.RA118.003279