Zinc in PDB 5y88: Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
Enzymatic activity of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
All present enzymatic activity of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom:
2.3.2.27;
3.6.4.13;
Other elements in 5y88:
The structure of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
(pdb code 5y88). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the
Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom, PDB code: 5y88:
Jump to Zinc binding site number:
1;
2;
3;
4;
5;
6;
Zinc binding site 1 out
of 6 in 5y88
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Zinc Binding Sites List in 5y88
Zinc binding site 1 out
of 6 in the Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
L:Zn1000
b:60.0
occ:1.00
|
SG
|
L:CYS108
|
2.2
|
56.4
|
1.0
|
SG
|
L:CYS148
|
2.2
|
58.4
|
1.0
|
CB
|
L:CYS104
|
2.2
|
55.5
|
1.0
|
SG
|
L:CYS105
|
2.3
|
85.3
|
1.0
|
SG
|
L:CYS104
|
2.3
|
74.3
|
1.0
|
CB
|
L:CYS148
|
3.1
|
54.1
|
1.0
|
CB
|
L:CYS105
|
3.3
|
60.5
|
1.0
|
CA
|
L:CYS104
|
3.6
|
53.8
|
1.0
|
N
|
L:CYS105
|
3.7
|
63.1
|
1.0
|
ZN
|
L:ZN1002
|
3.7
|
51.5
|
1.0
|
CB
|
L:CYS108
|
3.7
|
65.4
|
1.0
|
ZN
|
L:ZN1001
|
3.9
|
46.5
|
1.0
|
C
|
L:CYS104
|
3.9
|
60.2
|
1.0
|
CA
|
L:CYS105
|
4.1
|
59.9
|
1.0
|
SG
|
L:CYS122
|
4.3
|
55.9
|
1.0
|
SG
|
L:CYS145
|
4.4
|
45.7
|
1.0
|
CA
|
L:CYS148
|
4.4
|
55.9
|
1.0
|
CB
|
L:CYS150
|
4.6
|
73.6
|
1.0
|
O
|
L:CYS104
|
4.7
|
58.1
|
1.0
|
N
|
L:CYS104
|
4.7
|
51.6
|
1.0
|
N
|
L:CYS108
|
4.8
|
70.0
|
1.0
|
SG
|
L:CYS153
|
4.8
|
63.1
|
1.0
|
CA
|
L:CYS108
|
4.8
|
63.5
|
1.0
|
N
|
L:CYS148
|
4.9
|
58.1
|
1.0
|
|
Zinc binding site 2 out
of 6 in 5y88
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Zinc Binding Sites List in 5y88
Zinc binding site 2 out
of 6 in the Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
L:Zn1001
b:46.5
occ:1.00
|
SG
|
L:CYS153
|
2.1
|
63.1
|
1.0
|
SG
|
L:CYS122
|
2.2
|
55.9
|
1.0
|
SG
|
L:CYS104
|
2.3
|
74.3
|
1.0
|
SG
|
L:CYS150
|
2.3
|
96.8
|
1.0
|
CB
|
L:CYS150
|
2.4
|
73.6
|
1.0
|
CB
|
L:CYS104
|
3.2
|
55.5
|
1.0
|
CB
|
L:CYS153
|
3.4
|
71.3
|
1.0
|
CB
|
L:CYS122
|
3.7
|
53.4
|
1.0
|
O
|
L:CYS122
|
3.7
|
54.6
|
1.0
|
N
|
L:CYS153
|
3.9
|
66.9
|
1.0
|
ZN
|
L:ZN1000
|
3.9
|
60.0
|
1.0
|
CA
|
L:CYS150
|
3.9
|
67.0
|
1.0
|
CA
|
L:CYS153
|
4.0
|
65.6
|
1.0
|
ZN
|
L:ZN1002
|
4.0
|
51.5
|
1.0
|
CA
|
L:CYS104
|
4.1
|
53.8
|
1.0
|
N
|
L:CYS104
|
4.2
|
51.6
|
1.0
|
SG
|
L:CYS108
|
4.2
|
56.4
|
1.0
|
C
|
L:CYS122
|
4.3
|
50.7
|
1.0
|
CA
|
L:CYS122
|
4.5
|
50.4
|
1.0
|
C
|
L:CYS150
|
4.6
|
68.3
|
1.0
|
N
|
L:CYS150
|
4.7
|
62.1
|
1.0
|
CB
|
L:CYS108
|
4.9
|
65.4
|
1.0
|
N
|
L:ARG151
|
4.9
|
68.2
|
1.0
|
C
|
L:GLY152
|
5.0
|
66.6
|
1.0
|
|
Zinc binding site 3 out
of 6 in 5y88
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Zinc Binding Sites List in 5y88
Zinc binding site 3 out
of 6 in the Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
L:Zn1002
b:51.5
occ:1.00
|
SG
|
L:CYS145
|
2.1
|
45.7
|
1.0
|
SG
|
L:CYS120
|
2.3
|
69.2
|
1.0
|
SG
|
L:CYS108
|
2.3
|
56.4
|
1.0
|
SG
|
L:CYS122
|
2.4
|
55.9
|
1.0
|
CB
|
L:CYS122
|
2.8
|
53.4
|
1.0
|
CB
|
L:CYS108
|
3.1
|
65.4
|
1.0
|
CB
|
L:CYS120
|
3.2
|
51.6
|
1.0
|
CB
|
L:CYS145
|
3.3
|
54.0
|
1.0
|
ZN
|
L:ZN1000
|
3.7
|
60.0
|
1.0
|
SG
|
L:CYS148
|
3.8
|
58.4
|
1.0
|
CA
|
L:CYS108
|
3.9
|
63.5
|
1.0
|
ZN
|
L:ZN1001
|
4.0
|
46.5
|
1.0
|
CA
|
L:CYS122
|
4.3
|
50.4
|
1.0
|
CD2
|
L:HIS147
|
4.4
|
75.2
|
1.0
|
SG
|
L:CYS104
|
4.5
|
74.3
|
1.0
|
CA
|
L:CYS120
|
4.6
|
51.4
|
1.0
|
CA
|
L:CYS145
|
4.6
|
56.0
|
1.0
|
N
|
L:CYS108
|
4.8
|
70.0
|
1.0
|
CB
|
L:CYS150
|
4.8
|
73.6
|
1.0
|
N
|
L:CYS122
|
4.8
|
52.4
|
1.0
|
CB
|
L:CYS148
|
4.9
|
54.1
|
1.0
|
C
|
L:CYS108
|
4.9
|
55.8
|
1.0
|
O
|
L:CYS108
|
4.9
|
54.9
|
1.0
|
C
|
L:CYS122
|
4.9
|
50.7
|
1.0
|
CB
|
L:CYS104
|
5.0
|
55.5
|
1.0
|
|
Zinc binding site 4 out
of 6 in 5y88
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Zinc Binding Sites List in 5y88
Zinc binding site 4 out
of 6 in the Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
M:Zn400
b:0.5
occ:1.00
|
SG
|
M:CYS74
|
2.2
|
81.3
|
1.0
|
SG
|
M:CYS71
|
2.3
|
0.1
|
1.0
|
SG
|
M:CYS73
|
2.3
|
0.3
|
1.0
|
SG
|
M:CYS13
|
2.3
|
0.8
|
1.0
|
CB
|
M:CYS13
|
3.0
|
0.0
|
1.0
|
CB
|
M:CYS74
|
3.1
|
73.0
|
1.0
|
N
|
M:CYS74
|
3.3
|
72.4
|
1.0
|
CB
|
M:CYS16
|
3.4
|
0.4
|
1.0
|
N
|
M:CYS13
|
3.6
|
94.8
|
1.0
|
CB
|
M:CYS71
|
3.6
|
89.3
|
1.0
|
CA
|
M:CYS74
|
3.8
|
69.2
|
1.0
|
CA
|
M:CYS13
|
3.9
|
0.9
|
1.0
|
CB
|
M:CYS73
|
3.9
|
95.1
|
1.0
|
C
|
M:CYS73
|
4.2
|
72.6
|
1.0
|
N
|
M:CYS16
|
4.3
|
0.6
|
1.0
|
CG2
|
M:ILE12
|
4.4
|
0.8
|
1.0
|
CA
|
M:CYS73
|
4.4
|
79.7
|
1.0
|
CA
|
M:CYS16
|
4.5
|
0.5
|
1.0
|
N
|
M:CYS73
|
4.5
|
77.1
|
1.0
|
SG
|
M:CYS16
|
4.5
|
0.6
|
1.0
|
C
|
M:CYS13
|
4.6
|
0.8
|
1.0
|
C
|
M:ILE12
|
4.7
|
88.3
|
1.0
|
O
|
M:CYS13
|
4.7
|
0.2
|
1.0
|
CA
|
M:CYS71
|
4.9
|
78.3
|
1.0
|
|
Zinc binding site 5 out
of 6 in 5y88
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Zinc Binding Sites List in 5y88
Zinc binding site 5 out
of 6 in the Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 5 of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
M:Zn401
b:70.8
occ:1.00
|
SG
|
M:CYS64
|
2.1
|
63.6
|
1.0
|
SG
|
M:CYS34
|
2.3
|
62.6
|
1.0
|
SG
|
M:CYS61
|
2.4
|
94.3
|
1.0
|
SG
|
M:CYS37
|
2.4
|
76.3
|
1.0
|
CB
|
M:CYS61
|
2.9
|
69.4
|
1.0
|
CB
|
M:CYS37
|
3.0
|
58.6
|
1.0
|
CB
|
M:CYS34
|
3.2
|
56.1
|
1.0
|
CB
|
M:CYS64
|
3.3
|
68.6
|
1.0
|
N
|
M:CYS61
|
3.8
|
55.5
|
1.0
|
CA
|
M:CYS61
|
3.9
|
59.0
|
1.0
|
N
|
M:CYS37
|
3.9
|
61.8
|
1.0
|
CA
|
M:CYS37
|
4.0
|
53.7
|
1.0
|
N
|
M:CYS64
|
4.4
|
65.1
|
1.0
|
CA
|
M:CYS64
|
4.4
|
65.2
|
1.0
|
CA
|
M:CYS34
|
4.5
|
56.2
|
1.0
|
CB
|
M:SER114
|
4.8
|
79.1
|
1.0
|
NH1
|
M:ARG63
|
4.8
|
0.3
|
1.0
|
C
|
M:CYS37
|
4.8
|
46.4
|
1.0
|
N
|
M:CYS34
|
4.9
|
56.9
|
1.0
|
N
|
M:THR38
|
4.9
|
46.4
|
1.0
|
OG
|
M:SER114
|
4.9
|
78.8
|
1.0
|
O
|
M:LEU39
|
4.9
|
53.5
|
1.0
|
C
|
M:ILE60
|
5.0
|
51.9
|
1.0
|
|
Zinc binding site 6 out
of 6 in 5y88
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Zinc Binding Sites List in 5y88
Zinc binding site 6 out
of 6 in the Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 6 of Cryo-Em Structure of the Intron-Lariat Spliceosome Ready For Disassembly From S.Cerevisiae at 3.5 Angstrom within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
N:Zn400
b:36.1
occ:1.00
|
SG
|
N:CYS73
|
2.3
|
58.5
|
1.0
|
SG
|
N:CYS81
|
2.3
|
57.9
|
1.0
|
CE1
|
N:HIS91
|
2.3
|
76.0
|
1.0
|
SG
|
N:CYS87
|
2.3
|
55.6
|
1.0
|
NE2
|
N:HIS91
|
2.3
|
70.8
|
1.0
|
CB
|
N:CYS81
|
3.1
|
58.4
|
1.0
|
CB
|
N:CYS73
|
3.1
|
54.5
|
1.0
|
CB
|
N:CYS87
|
3.3
|
69.0
|
1.0
|
ND1
|
N:HIS91
|
3.5
|
69.3
|
1.0
|
CD2
|
N:HIS91
|
3.6
|
65.4
|
1.0
|
CG
|
N:HIS91
|
4.2
|
63.0
|
1.0
|
CB
|
N:PHE76
|
4.5
|
67.6
|
1.0
|
N
|
N:PHE76
|
4.5
|
57.4
|
1.0
|
CA
|
N:CYS81
|
4.6
|
59.6
|
1.0
|
N6
|
D:A35
|
4.6
|
54.2
|
1.0
|
CA
|
N:CYS73
|
4.6
|
53.2
|
1.0
|
CA
|
N:CYS87
|
4.7
|
68.7
|
1.0
|
O
|
N:TYR89
|
4.8
|
93.4
|
1.0
|
CB
|
N:PHE75
|
5.0
|
56.3
|
1.0
|
CA
|
N:PHE76
|
5.0
|
58.2
|
1.0
|
|
Reference:
R.Wan,
C.Yan,
R.Bai,
J.Lei,
Y.Shi.
Structure of An Intron Lariat Spliceosome From Saccharomyces Cerevisiae Cell(Cambridge,Mass.) V. 171 120 2017.
ISSN: ISSN 0092-8674
PubMed: 28919079
DOI: 10.1016/J.CELL.2017.08.029
Page generated: Mon Oct 28 15:28:21 2024
|