Zinc in PDB 5mtm: Monobody Mb(LCK_3) Bound to Lck-SH2 Domain

All present enzymatic activity of Monobody Mb(LCK_3) Bound to Lck-SH2 Domain:
2.7.10.2;

The structure of Monobody Mb(LCK_3) Bound to Lck-SH2 Domain, PDB code: 5mtm was solved by F.Pojer, T.Kukenshoner, S.Koide, O.Hantschel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.92 / 2.41
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	81.834, 81.834, 105.961, 90.00, 90.00, 90.00
R / Rfree (%)	20.5 / 25.7

The binding sites of Zinc atom in the Monobody Mb(LCK_3) Bound to Lck-SH2 Domain (pdb code 5mtm). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Monobody Mb(LCK_3) Bound to Lck-SH2 Domain, PDB code: 5mtm:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Monobody Mb(LCK_3) Bound to Lck-SH2 Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Monobody Mb(LCK_3) Bound to Lck-SH2 Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn101 b:63.7 occ:1.00 OE1 B:GLU40 2.1 61.7 1.0 OD2 B:ASP43 2.4 66.9 1.0 OD1 B:ASP43 2.9 68.6 1.0 CG B:ASP43 3.0 59.8 1.0 CD B:GLU40 3.3 65.8 1.0 CB B:GLU40 4.1 55.7 1.0 CG B:GLU40 4.1 58.4 1.0 OE2 B:GLU40 4.3 67.0 1.0 CB B:ASP43 4.5 67.1 1.0 O B:HOH201 4.9 57.7 1.0

Zinc binding site 2 out of 2 in the Monobody Mb(LCK_3) Bound to Lck-SH2 Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Monobody Mb(LCK_3) Bound to Lck-SH2 Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn102 b:63.9 occ:1.00 OE2 B:GLU11 2.3 72.5 1.0 OE1 B:GLU11 2.7 71.3 1.0 CD B:GLU11 2.8 73.2 1.0 CE B:LYS9 4.0 82.8 1.0 CG B:GLU11 4.3 72.1 1.0 O B:HOH203 4.6 59.4 1.0 CG B:LYS9 4.9 85.7 1.0 CD B:LYS9 5.0 85.8 1.0

T.Kukenshoner, N.E.Schmit, E.Bouda, F.Sha, F.Pojer, A.Koide, M.Seeliger, S.Koide, O.Hantschel. Selective Targeting of SH2 Domain-Phosphotyrosine Interactions of Src Family Tyrosine Kinases with Monobodies. J. Mol. Biol. V. 429 1364 2017.
ISSN: ESSN 1089-8638
PubMed: 28347651
DOI: 10.1016/J.JMB.2017.03.023