Zinc in PDB 5ix2: Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide

The structure of Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide, PDB code: 5ix2 was solved by S.Li, J.Du, D.J.Patel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.75 / 2.90
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	112.202, 147.542, 173.611, 90.00, 90.00, 90.00
R / Rfree (%)	22 / 23.6

The structure of Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide (pdb code 5ix2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide, PDB code: 5ix2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:92.9 occ:1.00 SG A:CYS416 2.4 90.4 1.0 SG A:CYS413 2.4 91.4 1.0 SG A:CYS446 2.5 90.2 1.0 SG A:CYS435 2.5 89.0 1.0 CB A:CYS435 2.6 86.5 1.0 N A:CYS435 2.9 89.8 1.0 CB A:CYS413 3.0 90.9 1.0 CA A:CYS435 3.3 87.4 1.0 CB A:CYS446 3.6 89.6 1.0 CA A:CYS446 3.7 91.8 1.0 CB A:CYS416 3.8 93.3 1.0 C A:TYR434 4.1 89.9 1.0 N A:CYS416 4.1 96.4 1.0 N A:CYS446 4.3 93.6 1.0 CA A:CYS413 4.4 92.4 1.0 CA A:TYR434 4.5 91.2 1.0 CA A:CYS416 4.5 96.1 1.0 C A:CYS435 4.5 86.2 1.0 C A:ALA415 4.6 0.8 1.0 N A:ALA415 4.8 97.8 1.0 N A:SER436 4.8 85.7 1.0 CB A:ALA415 5.0 97.5 1.0 C A:CYS446 5.0 88.7 1.0 C A:CYS413 5.0 96.1 1.0 CB A:TYR434 5.0 91.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Mouse MORC3 Atpase-Cw Cassette in Complex with Amppnp and Unmodified H3 Peptide within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn501 b:84.1 occ:1.00 SG B:CYS446 2.0 0.1 1.0 SG B:CYS416 2.1 0.1 1.0 CB B:CYS446 2.3 87.8 1.0 SG B:CYS413 2.4 87.2 1.0 CB B:CYS435 2.9 82.0 1.0 SG B:CYS435 3.0 93.5 1.0 CB B:CYS416 3.1 93.2 1.0 CB B:CYS413 3.1 87.2 1.0 CA B:CYS446 3.5 94.1 1.0 N B:CYS435 3.8 85.9 1.0 N B:CYS416 3.9 93.6 1.0 CA B:CYS435 4.0 81.3 1.0 N B:CYS446 4.0 94.8 1.0 CA B:CYS416 4.1 94.8 1.0 CA B:CYS413 4.6 91.0 1.0 CG B:LYS418 4.6 89.1 1.0 CB B:ALA415 4.7 94.8 1.0 C B:CYS446 4.7 95.1 1.0 NH2 B:ARG420 4.7 83.6 1.0 C B:CYS416 4.8 94.4 1.0 C B:ALA415 4.9 0.8 1.0 C B:ASN445 4.9 95.6 1.0 O B:CYS446 5.0 96.6 1.0 CB B:LYS418 5.0 87.2 1.0

S.Li, L.Yen, W.A.Pastor, J.B.Johnston, J.Du, C.J.Shew, W.Liu, J.Ho, B.Stender, A.T.Clark, A.L.Burlingame, L.Daxinger, D.J.Patel, S.E.Jacobsen. Mouse MORC3 Is A Ghkl Atpase That Localizes to H3K4ME3 Marked Chromatin Proc.Natl.Acad.Sci.Usa V. 113 E5108 2016.
ISSN: ESSN 1091-6490
PubMed: 27528681
DOI: 10.1073/PNAS.1609709113