Zinc in PDB 5b16: X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8.

All present enzymatic activity of X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8.:
3.1.26.3;

The structure of X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8., PDB code: 5b16 was solved by S.C.Kwon, T.A.Nguyen, Y.G.Choi, M.H.Jo, S.Hohng, V.N.Kim, J.S.Woo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.93 / 3.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	117.254, 118.136, 122.296, 90.00, 102.07, 90.00
R / Rfree (%)	26.7 / 30

The binding sites of Zinc atom in the X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8. (pdb code 5b16). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8., PDB code: 5b16:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1401 b:31.7 occ:1.00 NE2 A:HIS680 2.1 33.0 1.0 NE2 A:HIS609 2.1 30.7 1.0 SG A:CYS561 2.5 54.1 1.0 CE1 A:HIS609 2.5 30.1 1.0 SG A:CYS676 2.8 88.3 1.0 CD2 A:HIS680 2.9 34.0 1.0 CE1 A:HIS680 3.2 38.6 1.0 CD2 A:HIS609 3.4 30.3 1.0 CB A:CYS676 3.7 95.3 1.0 CB A:CYS561 3.7 83.7 1.0 ND1 A:HIS609 3.8 29.2 1.0 CG A:HIS680 4.1 40.0 1.0 CD2 A:LEU571 4.1 35.5 1.0 ND1 A:HIS680 4.2 43.2 1.0 CG A:HIS609 4.3 29.3 1.0 N A:CYS561 4.4 79.7 1.0 CG1 A:VAL642 4.5 59.3 1.0 CA A:CYS561 4.7 83.4 1.0 CD A:PRO677 4.8 64.2 1.0 CA A:CYS676 4.9 98.4 1.0 CG2 A:VAL642 5.0 48.4 1.0

Zinc binding site 2 out of 2 in the X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Structure of Drosha in Complex with the C-Terminal Tail of DGCR8. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1402 b:79.4 occ:1.00 NE2 A:HIS1026 2.3 73.3 1.0 NE2 A:HIS549 2.4 0.3 1.0 SG A:CYS538 2.5 0.3 1.0 CE1 A:HIS549 3.0 0.1 1.0 CE1 A:HIS1026 3.1 71.8 1.0 SG A:CYS536 3.1 89.6 1.0 CD2 A:HIS1026 3.3 72.6 1.0 CB A:CYS538 3.4 96.3 1.0 CD2 A:HIS549 3.5 0.9 1.0 ND1 A:HIS549 4.1 0.4 1.0 CB A:CYS536 4.2 68.9 1.0 ND1 A:HIS1026 4.3 75.7 1.0 CG A:HIS549 4.4 0.9 1.0 N A:CYS538 4.4 89.1 1.0 CG A:HIS1026 4.4 84.1 1.0 CA A:CYS538 4.5 95.5 1.0 CA A:CYS536 4.5 77.9 1.0 N A:LYS537 4.7 80.1 1.0 O A:ALA542 5.0 0.2 1.0

S.C.Kwon, T.A.Nguyen, Y.G.Choi, M.H.Jo, S.Hohng, V.N.Kim, J.S.Woo. Structure of Human Drosha Cell V. 164 81 2016.
ISSN: ISSN 1097-4172
PubMed: 26748718
DOI: 10.1016/J.CELL.2015.12.019