Zinc in PDB 4z7i: Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand

All present enzymatic activity of Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand:
3.4.11.3;

The structure of Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand, PDB code: 4z7i was solved by A.Mpakali, E.Saridakis, K.Harlos, Y.Zhao, E.Stratikos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	128.18 / 3.31
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	68.520, 256.350, 73.060, 90.00, 111.58, 90.00
R / Rfree (%)	21.1 / 27

The binding sites of Zinc atom in the Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand (pdb code 4z7i). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand, PDB code: 4z7i:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1101 b:86.7 occ:1.00 OE1 A:GLU487 2.1 86.0 1.0 NE2 A:HIS464 2.1 74.6 1.0 NE2 A:HIS468 2.1 96.8 1.0 O13 C:2X01 2.4 92.4 1.0 OE2 A:GLU487 2.5 74.9 1.0 O12 C:2X01 2.6 0.7 1.0 CD A:GLU487 2.6 85.2 1.0 CD2 A:HIS468 2.6 0.2 1.0 CD2 A:HIS464 3.0 89.1 1.0 P11 C:2X01 3.0 95.1 1.0 CE1 A:HIS464 3.1 74.0 1.0 CE1 A:HIS468 3.3 95.0 1.0 CG A:HIS468 3.9 90.2 1.0 N10 C:2X01 4.0 99.6 1.0 C3 C:2X01 4.1 96.8 1.0 CG A:GLU487 4.1 79.6 1.0 CG A:HIS464 4.1 90.2 1.0 ND1 A:HIS464 4.2 76.9 1.0 ND1 A:HIS468 4.2 94.3 1.0 CE2 A:TYR549 4.3 89.4 1.0 C1 C:4L82 4.3 0.5 1.0 CB A:ALA490 4.4 79.0 1.0 OE1 A:GLU431 4.4 0.1 1.0 CA C:4L82 4.4 0.2 1.0 OH A:TYR549 4.5 0.7 1.0 CZ A:TYR549 4.7 96.3 1.0 CB A:GLU487 4.8 74.6 1.0 OE2 A:GLU431 4.8 94.3 1.0 CD A:GLU431 4.8 0.7 1.0 CA A:GLU487 4.8 81.8 1.0 OE1 A:GLU465 4.9 0.9 1.0 O C:4L82 5.0 0.4 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Insulin Regulated Aminopeptidase in Complex with Ligand within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1101 b:0.3 occ:1.00 NE2 B:HIS464 2.1 0.6 1.0 NE2 B:HIS468 2.1 0.6 1.0 OE1 B:GLU487 2.1 0.9 1.0 O12 D:2X01 2.5 0.9 1.0 OE2 B:GLU487 2.5 0.7 1.0 CD B:GLU487 2.6 0.1 1.0 O13 D:2X01 2.7 0.9 1.0 CD2 B:HIS468 2.8 0.9 1.0 CD2 B:HIS464 2.9 0.5 1.0 P11 D:2X01 3.1 0.1 1.0 CE1 B:HIS464 3.2 0.9 1.0 CE1 B:HIS468 3.3 0.1 1.0 CG B:HIS468 4.0 0.9 1.0 CG B:HIS464 4.1 1.0 1.0 CG B:GLU487 4.1 0.2 1.0 N10 D:2X01 4.1 0.7 1.0 C3 D:2X01 4.2 0.7 1.0 ND1 B:HIS464 4.2 0.4 1.0 ND1 B:HIS468 4.2 0.7 1.0 C1 D:4L82 4.4 0.3 1.0 OE2 B:GLU431 4.5 0.7 1.0 OE1 B:GLU431 4.5 0.0 1.0 CD B:GLU431 4.5 0.1 1.0 CA D:4L82 4.6 0.2 1.0 CB B:ALA490 4.6 0.4 1.0 CE2 B:TYR549 4.7 0.6 1.0 CB B:GLU487 4.8 0.9 1.0 CA B:GLU487 4.8 0.5 1.0 CD1 D:4L82 4.9 0.3 1.0 OH B:TYR549 4.9 0.8 1.0 OE1 B:GLU465 4.9 0.0 1.0 O B:HIS464 5.0 0.7 1.0

A.Mpakali, E.Saridakis, K.Harlos, Y.Zhao, A.Papakyriakou, P.Kokkala, D.Georgiadis, E.Stratikos. Crystal Structure of Insulin-Regulated Aminopeptidase with Bound Substrate Analogue Provides Insight on Antigenic Epitope Precursor Recognition and Processing. J Immunol. V. 195 2842 2015.
ISSN: ESSN 1550-6606
PubMed: 26259583
DOI: 10.4049/JIMMUNOL.1501103