Zinc in PDB 4xwo: Structure of GET3 Bound to the Transmembrane Domain of SEC22

The structure of Structure of GET3 Bound to the Transmembrane Domain of SEC22, PDB code: 4xwo was solved by A.Mateja, M.Paduch, H.-Y.Chang, A.Szydlowska, A.A.Kossiakoff, R.S.Hegde, R.J.Keenan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.72 / 2.75
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	112.580, 119.450, 147.570, 71.89, 89.86, 66.61
R / Rfree (%)	19.6 / 24.7

The structure of Structure of GET3 Bound to the Transmembrane Domain of SEC22 also contains other interesting chemical elements:

Magnesium

(Mg)

8 atoms

The binding sites of Zinc atom in the Structure of GET3 Bound to the Transmembrane Domain of SEC22 (pdb code 4xwo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of GET3 Bound to the Transmembrane Domain of SEC22, PDB code: 4xwo:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Structure of GET3 Bound to the Transmembrane Domain of SEC22


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of GET3 Bound to the Transmembrane Domain of SEC22 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:65.5 occ:1.00 SG A:CYS288 2.3 70.4 1.0 SG B:CYS288 2.3 73.0 1.0 SG B:CYS285 2.4 82.1 1.0 SG A:CYS285 2.4 96.8 1.0 CB A:CYS285 3.1 94.9 1.0 CB B:CYS288 3.3 69.1 1.0 CB A:CYS288 3.3 69.2 1.0 CB B:CYS285 3.5 88.3 1.0 N B:CYS288 4.3 67.2 1.0 N A:CYS288 4.3 68.5 1.0 CA B:CYS288 4.4 64.5 1.0 CA A:CYS288 4.4 65.6 1.0 CA A:CYS285 4.6 94.5 1.0 CA B:CYS285 4.9 96.8 1.0

Zinc binding site 2 out of 4 in the Structure of GET3 Bound to the Transmembrane Domain of SEC22


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of GET3 Bound to the Transmembrane Domain of SEC22 within 5.0Å range: probe atom residue distance (Å) B Occ G:Zn402 b:67.8 occ:1.00 SG G:CYS285 2.3 91.0 1.0 SG H:CYS288 2.3 65.0 1.0 SG H:CYS285 2.3 88.5 1.0 SG G:CYS288 2.3 79.2 1.0 CB G:CYS285 3.1 97.8 1.0 CB H:CYS288 3.2 65.0 1.0 CB G:CYS288 3.4 77.4 1.0 CB H:CYS285 3.8 92.0 1.0 N H:CYS288 4.2 67.5 1.0 CA H:CYS288 4.3 62.5 1.0 N G:CYS288 4.5 71.2 1.0 CA G:CYS288 4.5 70.7 1.0 CA G:CYS285 4.6 0.2 1.0

Zinc binding site 3 out of 4 in the Structure of GET3 Bound to the Transmembrane Domain of SEC22


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of GET3 Bound to the Transmembrane Domain of SEC22 within 5.0Å range: probe atom residue distance (Å) B Occ M:Zn402 b:72.7 occ:1.00 SG M:CYS288 2.2 63.6 1.0 SG N:CYS288 2.2 81.0 1.0 SG M:CYS285 2.3 72.0 1.0 SG N:CYS285 2.3 59.8 1.0 CB M:CYS288 2.9 55.8 1.0 CB N:CYS288 3.1 73.2 1.0 CB M:CYS285 3.1 70.3 1.0 N N:CYS285 3.5 83.2 1.0 CB N:CYS285 3.6 70.6 1.0 CA N:CYS285 4.1 77.8 1.0 CA M:CYS288 4.2 55.3 1.0 N M:CYS288 4.3 55.3 1.0 CA N:CYS288 4.4 63.8 1.0 C N:ASN284 4.4 93.4 1.0 N N:CYS288 4.4 61.7 1.0 CA N:ASN284 4.4 0.8 1.0 CA M:CYS285 4.6 77.8 1.0 CB N:ASN284 4.8 95.0 1.0 C N:CYS285 5.0 72.0 1.0

Zinc binding site 4 out of 4 in the Structure of GET3 Bound to the Transmembrane Domain of SEC22


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of GET3 Bound to the Transmembrane Domain of SEC22 within 5.0Å range: probe atom residue distance (Å) B Occ S:Zn402 b:97.1 occ:1.00 SG T:CYS288 2.3 87.5 1.0 SG S:CYS288 2.3 82.8 1.0 SG T:CYS285 2.3 76.5 1.0 SG S:CYS285 2.3 97.7 1.0 CB S:CYS285 2.8 99.9 1.0 CB S:CYS288 3.3 86.0 1.0 CB T:CYS288 3.3 90.1 1.0 CB T:CYS285 3.7 81.0 1.0 N T:CYS285 3.8 84.3 1.0 N S:CYS288 4.1 83.5 1.0 CA S:CYS288 4.3 85.6 1.0 CA S:CYS285 4.3 0.5 1.0 CA T:CYS285 4.4 83.6 1.0 CA T:CYS288 4.5 86.6 1.0 N T:CYS288 4.5 84.9 1.0 C T:ASN284 4.8 89.9 1.0 CB T:ASN284 4.9 78.8 1.0 C S:CYS285 4.9 1.0 1.0 CA T:ASN284 4.9 90.4 1.0

A.Mateja, M.Paduch, H.Y.Chang, A.Szydlowska, A.A.Kossiakoff, R.S.Hegde, R.J.Keenan. Protein Targeting. Structure of the GET3 Targeting Factor in Complex with Its Membrane Protein Cargo. Science V. 347 1152 2015.
ISSN: ESSN 1095-9203
PubMed: 25745174
DOI: 10.1126/SCIENCE.1261671