Zinc in PDB 4wrn: Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein

The structure of Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein, PDB code: 4wrn was solved by M.Bokhove, D.De Sanctis, L.Jovine, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.98 / 3.20
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	242.320, 242.320, 258.860, 90.00, 90.00, 120.00
R / Rfree (%)	22.1 / 24.6

The binding sites of Zinc atom in the Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein (pdb code 4wrn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein, PDB code: 4wrn:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn804 b:0.7 occ:1.00 NE2 A:HIS24 2.0 1.0 1.0 OE2 A:GLU302 2.0 0.6 1.0 NE2 A:HIS20 2.0 1.0 1.0 OE1 A:GLU302 2.1 0.9 1.0 CD A:GLU302 2.3 0.2 1.0 CE1 A:HIS20 2.8 0.3 1.0 HE1 A:HIS20 2.9 0.7 1.0 CE1 A:HIS24 3.0 0.4 1.0 CD2 A:HIS24 3.0 0.2 1.0 HE1 A:HIS24 3.2 0.7 1.0 CD2 A:HIS20 3.2 0.1 1.0 HG22 A:THR55 3.2 0.7 1.0 HD2 A:HIS24 3.2 0.7 1.0 HD2 A:HIS20 3.4 0.7 1.0 HG21 A:THR55 3.7 0.7 1.0 CG A:GLU302 3.8 0.0 1.0 CG2 A:THR55 3.9 0.9 1.0 ND1 A:HIS20 4.0 0.9 1.0 HG3 A:GLU302 4.1 0.7 1.0 ND1 A:HIS24 4.1 0.6 1.0 CG A:HIS24 4.2 0.0 1.0 HG2 A:GLU302 4.2 0.7 1.0 CG A:HIS20 4.2 0.4 1.0 HG23 A:THR55 4.3 0.7 1.0 HB2 A:HIS23 4.3 0.7 1.0 HD1 A:HIS20 4.8 0.7 1.0 HE1 A:PHE51 4.8 0.7 1.0 HB2 A:GLU302 4.8 0.7 1.0 CB A:GLU302 4.8 0.8 1.0 HB3 A:GLU302 4.9 0.7 1.0 HD1 A:HIS24 4.9 0.7 1.0 HH A:TYR307 4.9 0.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Polymerization Region of Human Uromodulin/Tamm-Horsfall Protein within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn801 b:0.7 occ:1.00 OE2 B:GLU302 2.0 1.0 1.0 OE1 B:GLU302 2.1 0.9 1.0 NE2 B:HIS20 2.1 0.5 1.0 NE2 B:HIS24 2.1 0.5 1.0 CD B:GLU302 2.2 0.8 1.0 HE1 B:HIS20 2.5 0.7 1.0 CE1 B:HIS20 2.6 0.9 1.0 CE1 B:HIS24 3.0 0.0 1.0 CD2 B:HIS24 3.1 0.9 1.0 HE1 B:HIS24 3.2 0.7 1.0 HD2 B:HIS24 3.3 0.7 1.0 CD2 B:HIS20 3.3 0.0 1.0 CG B:GLU302 3.7 0.5 1.0 HD2 B:HIS20 3.7 0.7 1.0 ND1 B:HIS20 3.9 0.1 1.0 HG3 B:GLU302 3.9 0.7 1.0 HH B:TYR307 3.9 0.7 1.0 HG2 B:GLU302 4.0 0.7 1.0 ND1 B:HIS24 4.1 0.7 1.0 CG B:HIS24 4.2 0.9 1.0 HG1 B:THR55 4.2 0.7 1.0 CG B:HIS20 4.2 0.3 1.0 HB3 B:HIS23 4.5 0.7 1.0 OH B:TYR307 4.6 0.1 1.0 HB2 B:HIS23 4.6 0.7 1.0 HD21 B:ASN306 4.8 0.7 1.0 CB B:GLU302 4.8 0.3 1.0 HB2 B:GLU302 4.8 0.7 1.0 HD22 B:ASN306 4.9 0.7 1.0 HD1 B:HIS24 4.9 0.7 1.0 HB3 B:GLU302 4.9 0.7 1.0 OG1 B:THR55 4.9 0.8 1.0 HE1 B:TYR307 4.9 0.7 1.0 ND2 B:ASN306 5.0 0.2 1.0

M.Bokhove, K.Nishimura, M.Brunati, L.Han, D.De Sanctis, L.Rampoldi, L.Jovine. A Structured Interdomain Linker Directs Self-Polymerization of Human Uromodulin. Proc.Natl.Acad.Sci.Usa V. 113 1552 2016.
ISSN: ESSN 1091-6490
PubMed: 26811476
DOI: 10.1073/PNAS.1519803113