Zinc in PDB 4qts: Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex

The structure of Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex, PDB code: 4qts was solved by T.Numata, H.Inanaga, T.Osawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.46 / 3.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	74.653, 96.319, 194.797, 90.00, 90.00, 90.00
R / Rfree (%)	22.5 / 28.8

The binding sites of Zinc atom in the Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex (pdb code 4qts). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex, PDB code: 4qts:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:0.4 occ:1.00 ND1 C:HIS81 2.1 0.9 1.0 SG C:CYS89 2.4 0.8 1.0 CB C:CYS86 2.5 0.8 1.0 CE1 C:HIS81 2.5 0.0 1.0 SG C:CYS83 2.7 0.3 1.0 SG C:CYS86 2.8 0.2 1.0 CB C:CYS89 3.3 0.5 1.0 CG C:HIS81 3.4 1.0 1.0 NE2 C:HIS81 3.8 1.0 1.0 CB C:CYS83 4.0 0.9 1.0 CA C:CYS86 4.0 1.0 1.0 N C:CYS83 4.0 0.7 1.0 CB C:HIS81 4.1 0.9 1.0 CD2 C:HIS81 4.2 0.6 1.0 O C:CYS83 4.2 0.6 1.0 N C:CYS89 4.2 0.4 1.0 CA C:CYS83 4.4 0.4 1.0 CA C:CYS89 4.4 0.8 1.0 C C:CYS83 4.5 0.5 1.0 O C:GLU85 4.6 0.5 1.0 N C:CYS86 4.6 0.9 1.0 C C:CYS86 4.7 0.8 1.0 N C:ASP82 4.8 0.3 1.0 C C:GLU85 4.8 0.0 1.0 C C:ASP82 4.9 0.4 1.0 C C:HIS81 4.9 0.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn301 b:0.3 occ:1.00 ND1 D:HIS81 2.1 0.1 1.0 SG D:CYS89 2.6 0.8 1.0 SG D:CYS83 2.6 0.4 1.0 CE1 D:HIS81 2.7 0.1 1.0 CB D:CYS86 2.9 0.5 1.0 SG D:CYS86 3.0 0.1 1.0 CG D:HIS81 3.4 0.5 1.0 CB D:CYS89 3.6 0.6 1.0 NE2 D:HIS81 3.9 1.0 1.0 CB D:CYS83 3.9 0.6 1.0 CB D:HIS81 4.1 1.0 1.0 N D:CYS83 4.1 0.1 1.0 CD2 D:HIS81 4.2 0.3 1.0 CA D:CYS86 4.4 0.6 1.0 CA D:CYS83 4.4 0.4 1.0 O D:CYS83 4.5 0.9 1.0 O D:GLU85 4.6 1.0 1.0 C D:CYS83 4.7 0.1 1.0 CG1 D:ILE88 4.8 0.3 1.0 N D:CYS89 4.8 0.3 1.0 N D:ASP82 4.8 0.4 1.0 CA D:CYS89 4.8 0.6 1.0 C D:ASP82 4.9 0.2 1.0 N D:CYS86 5.0 0.1 1.0 C D:HIS81 5.0 0.8 1.0

T.Numata, H.Inanaga, C.Sato, T.Osawa. Crystal Structure of the CSM3-CSM4 Subcomplex in the Type III-A Crispr-Cas Interference Complex. J.Mol.Biol. 2014.
ISSN: ESSN 1089-8638
PubMed: 25451598
DOI: 10.1016/J.JMB.2014.09.029