Zinc in PDB 4o6a: Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna

All present enzymatic activity of Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna:
2.7.7.86;

The structure of Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna, PDB code: 4o6a was solved by X.Zhang, Z.Chen, X.W.Zhang, Z.J.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.66 / 1.86
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	183.711, 96.330, 75.989, 90.00, 98.28, 90.00
R / Rfree (%)	18.6 / 21.8

The binding sites of Zinc atom in the Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna (pdb code 4o6a). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna, PDB code: 4o6a:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:21.1 occ:1.00 NE2 A:HIS378 2.0 20.8 1.0 SG A:CYS384 2.3 21.3 1.0 SG A:CYS385 2.3 21.3 1.0 SG A:CYS392 2.4 19.8 1.0 CD2 A:HIS378 2.9 22.7 1.0 CE1 A:HIS378 3.1 20.7 1.0 CB A:CYS385 3.4 20.4 1.0 CB A:CYS392 3.4 23.0 1.0 CB A:CYS384 3.6 18.7 1.0 N A:CYS385 3.7 16.3 1.0 C A:CYS384 3.8 20.7 1.0 N A:CYS392 3.8 19.3 1.0 CG A:HIS378 4.1 18.5 1.0 CA A:CYS385 4.1 18.6 1.0 O A:CYS384 4.1 18.6 1.0 CA A:CYS392 4.1 19.4 1.0 NH1 A:ARG394 4.2 15.2 1.0 ND1 A:HIS378 4.2 20.1 1.0 CA A:CYS384 4.2 17.7 1.0 O A:HOH722 4.2 22.0 1.0 O A:HOH706 4.4 21.6 1.0 O A:ALA390 4.6 22.5 1.0 O A:HOH804 4.7 25.4 1.0 C A:CYS392 4.7 19.2 1.0 O A:HOH768 4.7 28.9 1.0 O A:CYS392 4.8 23.5 1.0 C A:LYS391 4.9 22.9 1.0

Zinc binding site 2 out of 2 in the Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mouse Cyclic Gmp-Amp Synthase (Cgas) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn601 b:25.1 occ:1.00 NE2 B:HIS378 2.1 22.9 1.0 SG B:CYS384 2.3 24.3 1.0 SG B:CYS385 2.3 24.1 1.0 SG B:CYS392 2.4 23.9 1.0 CD2 B:HIS378 2.9 21.9 1.0 CE1 B:HIS378 3.1 26.7 1.0 CB B:CYS392 3.3 23.6 1.0 CB B:CYS385 3.3 18.4 1.0 CB B:CYS384 3.6 22.2 1.0 N B:CYS385 3.6 20.0 1.0 C B:CYS384 3.7 25.7 1.0 N B:CYS392 3.8 27.3 1.0 CA B:CYS385 4.0 24.9 1.0 CA B:CYS392 4.1 26.0 1.0 O B:HOH1047 4.1 28.2 1.0 CG B:HIS378 4.1 22.9 1.0 O B:CYS384 4.1 20.9 1.0 CA B:CYS384 4.2 20.4 1.0 ND1 B:HIS378 4.2 23.3 1.0 NH1 B:ARG394 4.2 21.9 1.0 O B:HOH736 4.4 26.5 1.0 O B:HOH754 4.6 26.0 1.0 O B:ALA390 4.6 25.7 1.0 C B:CYS392 4.7 29.0 1.0 O B:CYS392 4.8 28.5 1.0 C B:LYS391 4.8 31.6 1.0 O C:HOH127 4.9 30.8 1.0

X.Zhang, J.Wu, F.Du, H.Xu, L.Sun, Z.Chen, C.A.Brautigam, X.Zhang, Z.J.Chen. The Cytosolic Dna Sensor Cgas Forms An Oligomeric Complex with Dna and Undergoes Switch-Like Conformational Changes in the Activation Loop. Cell Rep V. 6 421 2014.
ISSN: ESSN 2211-1247
PubMed: 24462292
DOI: 10.1016/J.CELREP.2014.01.003