Zinc in PDB 4msj: Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group

The structure of Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group, PDB code: 4msj was solved by R.K.Shrestha, J.A.Ronau, C.Das, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.31 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.799, 58.066, 187.500, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 23.7

The binding sites of Zinc atom in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group (pdb code 4msj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group, PDB code: 4msj:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn509 b:19.4 occ:1.00 OD2 A:ASP354 1.9 17.6 1.0 O A:HOH633 2.0 21.9 1.0 NE2 A:HIS343 2.0 19.8 1.0 NE2 A:HIS341 2.0 16.4 1.0 CG A:ASP354 2.7 17.8 1.0 OD1 A:ASP354 2.8 21.2 1.0 CD2 A:HIS343 2.9 16.8 1.0 CE1 A:HIS341 2.9 19.9 1.0 CD2 A:HIS341 3.1 17.2 1.0 CE1 A:HIS343 3.1 16.6 1.0 O A:HOH603 3.8 26.0 1.0 OE2 A:GLU286 3.8 22.1 1.0 O A:HOH679 3.9 29.6 1.0 CB A:ASP354 4.1 19.5 1.0 OG A:SER351 4.1 21.1 1.0 ND1 A:HIS341 4.1 14.8 1.0 CG A:HIS343 4.1 16.9 1.0 ND1 A:HIS343 4.2 20.4 1.0 CG A:HIS341 4.2 15.4 1.0 OE1 A:GLU286 4.3 21.3 1.0 O A:HOH690 4.3 42.3 1.0 CD A:GLU286 4.4 18.2 1.0 CB A:SER351 4.4 20.9 1.0 O A:PHE349 4.5 23.1 1.0 N A:SER351 4.5 20.1 1.0 CG1 A:VAL372 4.8 17.1 1.0

Zinc binding site 2 out of 6 in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn510 b:24.8 occ:1.00 NE2 A:HIS404 2.0 27.6 1.0 NE2 A:HIS406 2.1 24.3 1.0 NE2 A:HIS356 2.1 23.7 1.0 SG A:CYS397 2.3 25.5 1.0 CE1 A:HIS406 3.0 28.4 1.0 CD2 A:HIS404 3.0 26.5 1.0 CD2 A:HIS356 3.0 24.0 1.0 CD2 A:HIS406 3.1 23.6 1.0 CE1 A:HIS404 3.1 32.8 1.0 CE1 A:HIS356 3.1 21.5 1.0 CB A:CYS397 3.2 27.7 1.0 ND1 A:HIS406 4.1 26.2 1.0 CG A:HIS404 4.1 29.7 1.0 ND1 A:HIS404 4.1 26.5 1.0 CG A:HIS406 4.1 27.7 1.0 ND1 A:HIS356 4.2 22.2 1.0 CG A:HIS356 4.2 21.0 1.0 OG A:SER352 4.4 29.0 1.0 CD1 A:ILE394 4.5 23.1 1.0 CA A:CYS397 4.6 30.6 1.0 CB A:LYS399 4.8 26.6 1.0 C A:CYS397 4.8 33.1 1.0 O A:CYS397 4.8 29.6 1.0 CG A:LYS399 5.0 27.3 1.0

Zinc binding site 3 out of 6 in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn503 b:27.2 occ:1.00 OD2 B:ASP354 1.9 24.1 1.0 NE2 B:HIS343 2.1 27.1 1.0 NE2 B:HIS341 2.1 27.9 1.0 O B:HOH612 2.2 31.7 1.0 CG B:ASP354 2.7 23.8 1.0 OD1 B:ASP354 2.8 26.6 1.0 CD2 B:HIS343 3.0 24.4 1.0 CE1 B:HIS341 3.0 25.3 1.0 CE1 B:HIS343 3.1 28.4 1.0 CD2 B:HIS341 3.1 25.5 1.0 OG B:SER351 3.9 26.5 1.0 OE2 B:GLU286 4.0 30.2 1.0 CB B:ASP354 4.1 23.9 1.0 ND1 B:HIS341 4.1 25.1 1.0 CG B:HIS343 4.1 28.4 1.0 ND1 B:HIS343 4.2 26.0 1.0 CG B:HIS341 4.2 25.2 1.0 N B:SER351 4.3 24.4 1.0 CB B:SER351 4.4 30.4 1.0 O B:PHE349 4.4 26.6 1.0 OE1 B:GLU286 4.4 32.9 1.0 CD B:GLU286 4.6 32.9 1.0 O B:HOH650 4.8 38.1 1.0 CG1 B:VAL372 4.9 22.1 1.0

Zinc binding site 4 out of 6 in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn504 b:31.8 occ:1.00 NE2 B:HIS356 2.1 29.8 1.0 NE2 B:HIS404 2.1 38.7 1.0 NE2 B:HIS406 2.1 30.2 1.0 SG B:CYS397 2.3 31.9 1.0 CD2 B:HIS404 2.9 38.8 1.0 CE1 B:HIS356 2.9 31.9 1.0 CD2 B:HIS406 3.0 30.2 1.0 CE1 B:HIS406 3.1 32.3 1.0 CB B:CYS397 3.2 33.7 1.0 CD2 B:HIS356 3.2 31.7 1.0 CE1 B:HIS404 3.2 36.4 1.0 ND1 B:HIS356 4.1 29.2 1.0 CG B:HIS404 4.1 40.0 1.0 CG B:HIS406 4.2 30.6 1.0 ND1 B:HIS406 4.2 27.8 1.0 ND1 B:HIS404 4.2 42.4 1.0 CG B:HIS356 4.2 27.1 1.0 OG B:SER352 4.3 34.6 1.0 CA B:CYS397 4.5 31.8 1.0 O B:CYS397 4.6 34.0 1.0 C B:CYS397 4.7 34.0 1.0 CB B:LYS399 4.8 43.4 1.0 NZ B:LYS399 4.8 46.2 1.0 CD1 B:ILE394 4.9 28.2 1.0 O B:LYS399 4.9 43.8 1.0 N B:LYS399 4.9 43.2 1.0

Zinc binding site 5 out of 6 in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn503 b:41.4 occ:1.00 OD2 C:ASP354 1.7 33.2 1.0 NE2 C:HIS343 1.9 39.3 1.0 NE2 C:HIS341 2.1 33.5 1.0 O C:HOH601 2.2 43.8 1.0 CG C:ASP354 2.6 40.3 1.0 OD1 C:ASP354 2.9 38.7 1.0 CE1 C:HIS343 2.9 40.7 1.0 CD2 C:HIS343 2.9 38.5 1.0 CD2 C:HIS341 3.0 39.4 1.0 CE1 C:HIS341 3.2 38.0 1.0 OE2 C:GLU286 3.8 49.5 1.0 ND1 C:HIS343 4.0 37.0 1.0 CB C:ASP354 4.1 36.8 1.0 CG C:HIS343 4.1 40.8 1.0 OG C:SER351 4.2 49.3 1.0 CG C:HIS341 4.2 38.7 1.0 ND1 C:HIS341 4.3 37.0 1.0 O C:PHE349 4.4 41.4 1.0 CD C:GLU286 4.5 50.3 1.0 OE1 C:GLU286 4.5 48.4 1.0 CB C:SER351 4.5 42.6 1.0 N C:SER351 4.5 41.9 1.0 O C:THR342 4.9 45.1 1.0 CG1 C:VAL372 5.0 39.2 1.0

Zinc binding site 6 out of 6 in the Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of S. Pombe Amsh-Like Protease SST2 Catalytic Domain From P212121 Space Group within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn504 b:45.8 occ:1.00 NE2 C:HIS406 1.9 49.4 1.0 NE2 C:HIS404 2.0 51.3 1.0 CE1 C:HIS406 2.1 50.8 1.0 NE2 C:HIS356 2.1 44.2 1.0 SG C:CYS397 2.4 44.2 1.0 CE1 C:HIS404 3.0 51.4 1.0 CD2 C:HIS356 3.0 44.2 1.0 CD2 C:HIS404 3.0 50.8 1.0 CE1 C:HIS356 3.1 46.4 1.0 CD2 C:HIS406 3.2 49.1 1.0 ND1 C:HIS406 3.3 51.7 1.0 CB C:CYS397 3.5 44.5 1.0 CG C:HIS406 3.9 54.1 1.0 ND1 C:HIS404 4.1 53.5 1.0 CG C:HIS404 4.2 54.0 1.0 CG C:HIS356 4.2 40.4 1.0 ND1 C:HIS356 4.2 38.5 1.0 OG C:SER352 4.4 44.8 1.0 CB C:LYS399 4.9 51.6 1.0 CA C:CYS397 4.9 49.8 1.0 O C:LYS399 5.0 56.7 1.0

R.K.Shrestha, J.A.Ronau, C.W.Davies, R.G.Guenette, E.R.Strieter, L.N.Paul, C.Das. Insights Into the Mechanism of Deubiquitination By Jamm Deubiquitinases From Cocrystal Structures of the Enzyme with the Substrate and Product. Biochemistry V. 53 3199 2014.
ISSN: ISSN 0006-2960
PubMed: 24787148
DOI: 10.1021/BI5003162