Zinc in PDB 4m1c: Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40)

All present enzymatic activity of Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40):
3.4.24.56;

The structure of Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40), PDB code: 4m1c was solved by L.M.Mccord, W.Liang, M.Farcasanu, K.Scherpelz, S.C.Meredith, S.Koide, W.J.Tang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.06 / 3.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.583, 135.324, 368.524, 90.00, 90.00, 90.00
R / Rfree (%)	22.8 / 27

The binding sites of Zinc atom in the Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40) (pdb code 4m1c). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40), PDB code: 4m1c:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1101 b:68.3 occ:1.00 NE2 A:HIS112 2.1 90.6 1.0 O G:PHE19 2.2 0.4 1.0 OE1 A:GLU189 2.3 88.5 1.0 CE1 A:HIS108 2.3 84.8 1.0 NE2 A:HIS108 2.3 81.3 1.0 CE1 A:HIS112 2.4 94.8 1.0 OE2 A:GLU189 2.7 92.1 1.0 CD A:GLU189 2.8 90.5 1.0 C G:PHE19 3.1 0.5 1.0 CD2 A:HIS112 3.5 90.1 1.0 ND1 A:HIS108 3.6 87.0 1.0 CD2 A:HIS108 3.6 80.7 1.0 ND1 A:HIS112 3.7 97.1 1.0 CA G:PHE20 3.7 0.8 1.0 N G:PHE20 3.8 0.2 1.0 O G:VAL18 4.2 0.6 1.0 CG A:HIS112 4.2 92.3 1.0 CG A:HIS108 4.2 83.8 1.0 CA G:PHE19 4.2 0.9 1.0 CG A:GLU189 4.2 89.4 1.0 N G:PHE19 4.3 0.7 1.0 C G:VAL18 4.3 0.5 1.0 NE2 A:GLN111 4.3 84.1 1.0 C G:PHE20 4.6 0.2 1.0 CB G:PHE20 4.7 0.6 1.0 CB G:VAL18 4.8 94.0 1.0 O G:PHE20 4.9 0.8 1.0 CB A:GLU189 4.9 84.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2001 b:59.3 occ:1.00 NE2 B:HIS112 2.1 80.8 1.0 CE1 B:HIS112 2.3 87.9 1.0 OE1 B:GLU189 2.4 72.5 1.0 CE1 B:HIS108 2.4 87.2 1.0 NE2 B:HIS108 2.5 85.5 1.0 OE2 B:GLU189 2.6 70.1 1.0 CD B:GLU189 2.8 70.1 1.0 CD2 B:HIS112 3.2 76.2 1.0 ND1 B:HIS108 3.4 85.0 1.0 ND1 B:HIS112 3.4 88.0 1.0 CD2 B:HIS108 3.6 82.2 1.0 NE2 B:GLN111 3.8 83.6 1.0 CG B:HIS112 3.9 78.2 1.0 CG B:HIS108 4.0 82.2 1.0 O B:HIS108 4.3 79.3 1.0 CG B:GLU189 4.4 67.9 1.0 CD B:GLN111 4.5 80.2 1.0 OE1 B:GLN111 4.6 84.6 1.0 OH B:TYR831 4.9 62.8 1.0 CE1 B:TYR831 4.9 55.5 1.0

L.A.Mccord, W.Liang, M.Farcasanu, K.Scherpelz, S.C.Meredith, S.Koide, W.J.Tang. Crystal Structure Analysis of Fab-Bound Human Insulin Degrading Enzyme (Ide) in Complex with Amyloid-Beta (1-40) To Be Published.