Zinc in PDB 4lo9: Human P53 Core Domain Mutant N235K
Protein crystallography data
The structure of Human P53 Core Domain Mutant N235K, PDB code: 4lo9
was solved by
B.D.Wallentine,
Y.Wang,
H.Luecke,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
53.38 /
2.50
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
69.164,
69.213,
83.851,
90.00,
90.11,
90.00
|
R / Rfree (%)
|
17.6 /
24.7
|
Zinc Binding Sites:
The binding sites of Zinc atom in the Human P53 Core Domain Mutant N235K
(pdb code 4lo9). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the
Human P53 Core Domain Mutant N235K, PDB code: 4lo9:
Jump to Zinc binding site number:
1;
2;
3;
4;
Zinc binding site 1 out
of 4 in 4lo9
Go back to
Zinc Binding Sites List in 4lo9
Zinc binding site 1 out
of 4 in the Human P53 Core Domain Mutant N235K
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Human P53 Core Domain Mutant N235K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn401
b:40.6
occ:1.00
|
ND1
|
A:HIS179
|
1.9
|
36.3
|
1.0
|
SG
|
A:CYS238
|
2.4
|
54.3
|
1.0
|
SG
|
A:CYS176
|
2.4
|
45.2
|
1.0
|
SG
|
A:CYS242
|
2.5
|
45.2
|
1.0
|
CE1
|
A:HIS179
|
2.8
|
37.6
|
1.0
|
CG
|
A:HIS179
|
3.0
|
44.7
|
1.0
|
CB
|
A:CYS242
|
3.3
|
53.0
|
1.0
|
CB
|
A:CYS176
|
3.3
|
46.5
|
1.0
|
CB
|
A:HIS179
|
3.5
|
43.0
|
1.0
|
CB
|
A:CYS238
|
3.7
|
36.0
|
1.0
|
NE2
|
A:HIS179
|
3.9
|
43.9
|
1.0
|
N
|
A:CYS176
|
3.9
|
33.4
|
1.0
|
CA
|
A:CYS238
|
4.0
|
46.0
|
1.0
|
CD2
|
A:HIS179
|
4.0
|
44.6
|
1.0
|
CA
|
A:CYS176
|
4.2
|
41.5
|
1.0
|
N
|
A:ASN239
|
4.2
|
44.4
|
1.0
|
N
|
A:HIS179
|
4.4
|
44.3
|
1.0
|
O
|
A:HOH519
|
4.6
|
45.9
|
1.0
|
CA
|
A:HIS179
|
4.6
|
43.3
|
1.0
|
C
|
A:CYS238
|
4.6
|
36.2
|
1.0
|
CA
|
A:CYS242
|
4.7
|
57.0
|
1.0
|
O
|
A:CYS176
|
4.7
|
36.3
|
1.0
|
C
|
A:CYS176
|
4.8
|
35.6
|
1.0
|
O
|
A:MET237
|
5.0
|
36.9
|
1.0
|
|
Zinc binding site 2 out
of 4 in 4lo9
Go back to
Zinc Binding Sites List in 4lo9
Zinc binding site 2 out
of 4 in the Human P53 Core Domain Mutant N235K
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Human P53 Core Domain Mutant N235K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn401
b:43.0
occ:1.00
|
ND1
|
B:HIS179
|
1.9
|
37.8
|
1.0
|
SG
|
B:CYS242
|
2.4
|
42.4
|
1.0
|
SG
|
B:CYS238
|
2.4
|
48.0
|
1.0
|
SG
|
B:CYS176
|
2.4
|
38.7
|
1.0
|
CE1
|
B:HIS179
|
2.7
|
50.1
|
1.0
|
CG
|
B:HIS179
|
3.0
|
45.9
|
1.0
|
CB
|
B:CYS242
|
3.2
|
54.6
|
1.0
|
CB
|
B:CYS176
|
3.4
|
38.1
|
1.0
|
CB
|
B:HIS179
|
3.6
|
36.6
|
1.0
|
CB
|
B:CYS238
|
3.7
|
42.7
|
1.0
|
NE2
|
B:HIS179
|
3.9
|
50.1
|
1.0
|
CA
|
B:CYS238
|
4.0
|
42.1
|
1.0
|
N
|
B:CYS176
|
4.0
|
35.4
|
1.0
|
CD2
|
B:HIS179
|
4.1
|
45.9
|
1.0
|
N
|
B:ASN239
|
4.2
|
48.4
|
1.0
|
CA
|
B:CYS176
|
4.3
|
37.0
|
1.0
|
N
|
B:HIS179
|
4.4
|
38.5
|
1.0
|
C
|
B:CYS238
|
4.6
|
40.8
|
1.0
|
CA
|
B:CYS242
|
4.6
|
57.2
|
1.0
|
CA
|
B:HIS179
|
4.6
|
40.2
|
1.0
|
O
|
B:CYS176
|
4.9
|
41.2
|
1.0
|
O
|
B:MET237
|
4.9
|
29.8
|
1.0
|
C
|
B:CYS176
|
5.0
|
38.0
|
1.0
|
CD2
|
B:HIS178
|
5.0
|
45.6
|
1.0
|
|
Zinc binding site 3 out
of 4 in 4lo9
Go back to
Zinc Binding Sites List in 4lo9
Zinc binding site 3 out
of 4 in the Human P53 Core Domain Mutant N235K
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Human P53 Core Domain Mutant N235K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Zn401
b:52.1
occ:1.00
|
ND1
|
C:HIS179
|
1.9
|
32.6
|
1.0
|
SG
|
C:CYS242
|
2.3
|
45.2
|
1.0
|
SG
|
C:CYS238
|
2.4
|
56.6
|
1.0
|
CE1
|
C:HIS179
|
2.5
|
41.3
|
1.0
|
SG
|
C:CYS176
|
2.5
|
37.4
|
1.0
|
CB
|
C:CYS242
|
3.0
|
50.2
|
1.0
|
CG
|
C:HIS179
|
3.1
|
42.9
|
1.0
|
CB
|
C:CYS176
|
3.3
|
42.6
|
1.0
|
NE2
|
C:HIS179
|
3.7
|
40.7
|
1.0
|
CB
|
C:CYS238
|
3.8
|
42.5
|
1.0
|
CB
|
C:HIS179
|
3.8
|
43.3
|
1.0
|
CA
|
C:CYS238
|
4.0
|
40.2
|
1.0
|
N
|
C:CYS176
|
4.0
|
40.9
|
1.0
|
CD2
|
C:HIS179
|
4.0
|
44.2
|
1.0
|
N
|
C:ASN239
|
4.1
|
37.4
|
1.0
|
CA
|
C:CYS176
|
4.3
|
42.2
|
1.0
|
CA
|
C:CYS242
|
4.5
|
52.4
|
1.0
|
C
|
C:CYS238
|
4.5
|
39.0
|
1.0
|
N
|
C:HIS179
|
4.7
|
47.9
|
1.0
|
O
|
C:ASN239
|
4.8
|
51.9
|
1.0
|
CA
|
C:HIS179
|
4.9
|
47.8
|
1.0
|
O
|
C:CYS176
|
4.9
|
39.2
|
1.0
|
C
|
C:CYS176
|
5.0
|
42.4
|
1.0
|
|
Zinc binding site 4 out
of 4 in 4lo9
Go back to
Zinc Binding Sites List in 4lo9
Zinc binding site 4 out
of 4 in the Human P53 Core Domain Mutant N235K
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Human P53 Core Domain Mutant N235K within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Zn401
b:56.8
occ:1.00
|
ND1
|
D:HIS179
|
1.9
|
45.5
|
1.0
|
SG
|
D:CYS242
|
2.3
|
53.9
|
1.0
|
SG
|
D:CYS176
|
2.5
|
48.5
|
1.0
|
SG
|
D:CYS238
|
2.5
|
55.6
|
1.0
|
CE1
|
D:HIS179
|
2.5
|
48.9
|
1.0
|
CB
|
D:CYS242
|
3.0
|
57.7
|
1.0
|
CG
|
D:HIS179
|
3.1
|
51.6
|
1.0
|
CB
|
D:CYS176
|
3.1
|
59.8
|
1.0
|
NE2
|
D:HIS179
|
3.7
|
51.9
|
1.0
|
CB
|
D:CYS238
|
3.7
|
37.9
|
1.0
|
CB
|
D:HIS179
|
3.8
|
49.2
|
1.0
|
CA
|
D:CYS238
|
3.9
|
37.9
|
1.0
|
N
|
D:CYS176
|
4.0
|
50.1
|
1.0
|
CD2
|
D:HIS179
|
4.0
|
53.5
|
1.0
|
CA
|
D:CYS176
|
4.1
|
55.0
|
1.0
|
N
|
D:ASN239
|
4.1
|
42.9
|
1.0
|
CA
|
D:CYS242
|
4.5
|
59.9
|
1.0
|
C
|
D:CYS238
|
4.5
|
42.1
|
1.0
|
N
|
D:HIS179
|
4.6
|
50.4
|
1.0
|
CA
|
D:HIS179
|
4.8
|
50.9
|
1.0
|
O
|
D:ASN239
|
4.9
|
50.9
|
1.0
|
C
|
D:CYS176
|
4.9
|
51.7
|
1.0
|
O
|
D:MET237
|
4.9
|
39.4
|
1.0
|
|
Reference:
B.D.Wallentine,
Y.Wang,
V.Tretyachenko-Ladokhina,
M.Tan,
D.F.Senear,
H.Luecke.
Structures of Oncogenic, Suppressor and Rescued P53 Core-Domain Variants: Mechanisms of Mutant P53 Rescue. Acta Crystallogr.,Sect.D V. 69 2146 2013.
ISSN: ISSN 0907-4449
PubMed: 24100332
DOI: 10.1107/S0907444913020830
Page generated: Sun Oct 27 02:02:45 2024
|