Zinc in PDB 4j1l: Mutant Endotoxin Tent

All present enzymatic activity of Mutant Endotoxin Tent:
3.4.24.68;

The structure of Mutant Endotoxin Tent, PDB code: 4j1l was solved by J.Guo, X.Pan, Y.Zhao, S.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.12 / 2.60
Space group	C 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	105.380, 176.830, 57.360, 90.00, 90.00, 90.00
R / Rfree (%)	23.8 / 26.6

The binding sites of Zinc atom in the Mutant Endotoxin Tent (pdb code 4j1l). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Mutant Endotoxin Tent, PDB code: 4j1l:

Zinc binding site 1 out of 1 in the Mutant Endotoxin Tent


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mutant Endotoxin Tent within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:38.7 occ:1.00 OE2 A:GLU271 1.5 33.7 1.0 NE2 A:HIS237 2.2 18.3 1.0 NE2 A:HIS233 2.3 17.0 1.0 CD A:GLU271 2.4 36.6 1.0 OE1 A:GLU271 2.8 42.5 1.0 CD2 A:HIS237 3.0 16.5 1.0 CD2 A:HIS233 3.1 18.9 1.0 CE1 A:HIS237 3.2 18.6 1.0 O A:HOH665 3.3 28.4 1.0 CE1 A:HIS233 3.5 16.7 1.0 CG A:GLU271 3.7 31.0 1.0 OE2 A:GLU234 4.2 31.5 1.0 OH A:TYR375 4.3 63.5 1.0 CG A:HIS237 4.3 16.9 1.0 ND1 A:HIS237 4.3 17.2 1.0 CG A:HIS233 4.3 19.5 1.0 OE1 A:GLU234 4.3 27.3 1.0 ND1 A:HIS233 4.5 20.9 1.0 CD A:GLU234 4.7 25.2 1.0 CG2 A:THR274 4.7 18.4 1.0 CB A:GLU271 4.9 26.1 1.0

J.Guo, X.Pan, Y.Zhao, S.Chen. Engineering Clostridia Neurotoxins with Elevated Catalytic Activity Toxicon V. 74 158 2013.
ISSN: ISSN 0041-0101
PubMed: 23994593
DOI: 10.1016/J.TOXICON.2013.08.055