Zinc in PDB 4ixn: Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase

The structure of Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase, PDB code: 4ixn was solved by M.Jost, C.L.Drennan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	75.30 / 2.05
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	56.071, 68.436, 77.699, 90.00, 104.23, 90.00
R / Rfree (%)	20.3 / 23.8

The binding sites of Zinc atom in the Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase (pdb code 4ixn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase, PDB code: 4ixn:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:36.9 occ:1.00 NE2 B:HIS114 2.0 37.2 1.0 OE1 A:GLU74 2.0 37.2 1.0 NE2 A:HIS114 2.1 45.0 1.0 OE1 B:GLU74 2.1 37.2 1.0 OE2 B:GLU74 2.7 35.5 1.0 CD B:GLU74 2.7 36.3 1.0 CD A:GLU74 2.8 37.3 1.0 CE1 B:HIS114 2.8 41.8 1.0 OE2 A:GLU74 2.9 39.2 1.0 CE1 A:HIS114 3.1 43.6 1.0 CD2 A:HIS114 3.1 39.6 1.0 CD2 B:HIS114 3.1 34.5 1.0 ND1 B:HIS114 4.0 40.8 1.0 CG B:HIS114 4.2 37.7 1.0 ND1 A:HIS114 4.2 46.1 1.0 CG A:HIS114 4.2 43.9 1.0 CG A:GLU74 4.2 40.2 1.0 O B:HOH572 4.2 42.4 1.0 CG B:GLU74 4.2 33.1 1.0 OG A:SER113 4.6 42.4 1.0 CB A:SER113 4.6 39.9 1.0 O B:HOH532 4.8 41.9 1.0 OG B:SER113 4.8 34.8 1.0 O B:HOH533 4.9 45.6 1.0 CB B:SER113 4.9 35.4 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:68.9 occ:1.00 ND1 A:HIS170 2.6 57.4 1.0 OE1 A:GLU167 2.9 83.2 1.0 OE2 A:GLU167 3.3 83.2 1.0 CB A:HIS170 3.3 43.8 1.0 CG A:HIS170 3.3 52.0 1.0 CD A:GLU167 3.5 82.2 1.0 CE1 A:HIS170 3.7 59.8 1.0 CD2 A:HIS170 4.5 55.6 1.0 NE2 A:HIS170 4.7 58.8 1.0 CA A:HIS170 4.8 38.8 1.0 CG A:GLU167 4.9 79.8 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Zn(II)-Bound E37A,C66A,C67A Triple Mutant Yjia Gtpase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn403 b:78.0 occ:1.00 NE2 A:HIS187 2.1 0.1 1.0 CD2 A:HIS187 2.7 0.8 1.0 CE1 A:HIS187 3.3 0.0 1.0 CG A:HIS187 4.0 0.5 1.0 ND1 A:HIS187 4.2 0.9 1.0 CG2 A:THR186 4.5 91.9 1.0 CB A:THR186 4.7 90.9 1.0

A.M.Sydor, M.Jost, K.S.Ryan, K.E.Turo, C.D.Douglas, C.L.Drennan, D.B.Zamble. Metal Binding Properties of Escherichia Coli Yjia, A Member of the Metal Homeostasis-Associated COG0523 Family of Gtpases. Biochemistry V. 52 1788 2013.
ISSN: ISSN 0006-2960
PubMed: 24449932
DOI: 10.1021/BI301600Z