Zinc in PDB 4h0f: Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae

The structure of Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae, PDB code: 4h0f was solved by P.Karthe, R.Preethi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	42.400, 93.990, 66.880, 90.00, 105.42, 90.00
R / Rfree (%)	24 / 28.1

The binding sites of Zinc atom in the Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae (pdb code 4h0f). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae, PDB code: 4h0f:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:26.2 occ:1.00 OE2 B:GLU281 2.0 20.2 1.0 NE2 B:HIS206 2.1 21.5 1.0 NE2 B:HIS66 2.1 34.0 1.0 NE2 B:HIS142 2.2 17.7 1.0 CD B:GLU281 2.8 18.0 1.0 CD2 B:HIS206 3.0 23.2 1.0 OE1 B:GLU281 3.0 15.9 1.0 CD2 B:HIS142 3.0 18.9 1.0 CD2 B:HIS66 3.1 34.1 1.0 CE1 B:HIS206 3.1 22.9 1.0 CE1 B:HIS66 3.1 34.5 1.0 CE1 B:HIS142 3.2 14.3 1.0 O B:ILE226 3.3 35.1 1.0 CB B:ALA208 3.8 23.1 1.0 CG B:GLU281 4.1 16.3 1.0 CG B:HIS206 4.2 24.0 1.0 ND1 B:HIS206 4.2 25.1 1.0 CG B:HIS142 4.2 17.6 1.0 ND1 B:HIS142 4.2 18.6 1.0 ND1 B:HIS66 4.2 35.4 1.0 CG B:HIS66 4.2 34.1 1.0 C B:ILE226 4.5 36.0 1.0 CA B:ALA208 4.6 23.3 1.0 CD B:PRO228 4.8 35.9 1.0 O B:THR207 5.0 25.0 1.0

Zinc binding site 2 out of 2 in the Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mutant Structure of Laminin-Binding Adhesin (Lmb) From Streptococcus Agalactiae within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:21.6 occ:1.00 NE2 A:HIS66 1.9 27.5 1.0 OE2 A:GLU281 2.0 25.6 1.0 NE2 A:HIS142 2.2 19.9 1.0 NE2 A:HIS206 2.3 32.9 1.0 CD A:GLU281 2.8 23.5 1.0 CD2 A:HIS66 2.9 29.3 1.0 CE1 A:HIS66 2.9 30.2 1.0 CD2 A:HIS142 2.9 21.2 1.0 OE1 A:GLU281 2.9 22.3 1.0 CD2 A:HIS206 3.2 33.0 1.0 CE1 A:HIS206 3.3 32.3 1.0 CE1 A:HIS142 3.3 16.9 1.0 O A:ILE226 3.5 36.2 1.0 ND1 A:HIS66 4.0 30.5 1.0 CB A:ALA208 4.0 24.2 1.0 CG A:HIS66 4.0 30.8 1.0 CG A:HIS142 4.2 18.7 1.0 CG A:GLU281 4.2 25.8 1.0 ND1 A:HIS142 4.3 19.4 1.0 ND1 A:HIS206 4.4 34.1 1.0 CG A:HIS206 4.4 33.6 1.0 C A:ILE226 4.7 36.2 1.0 CD A:PRO228 4.8 40.7 1.0 CA A:ALA208 4.9 24.0 1.0

P.Ragunathan, D.Sridaran, A.Weigel, S.Shabayek, B.Spellerberg, K.Ponnuraj. Metal Binding Is Critical For the Folding and Function of Laminin Binding Protein, Lmb of Streptococcus Agalactiae. Plos One V. 8 67517 2013.
ISSN: ESSN 1932-6203
PubMed: 23826314
DOI: 10.1371/JOURNAL.PONE.0067517