Zinc in PDB 4gx2: Gsuk Channel Bound to Nad

The structure of Gsuk Channel Bound to Nad, PDB code: 4gx2 was solved by C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.14 / 3.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	232.548, 111.104, 164.561, 90.00, 134.78, 90.00
R / Rfree (%)	23 / 27

The structure of Gsuk Channel Bound to Nad also contains other interesting chemical elements:

Potassium	(K)	12 atoms
Calcium	(Ca)	4 atoms

The binding sites of Zinc atom in the Gsuk Channel Bound to Nad (pdb code 4gx2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Gsuk Channel Bound to Nad, PDB code: 4gx2:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn606 b:91.7 occ:1.00 NE2 A:HIS359 2.6 96.6 1.0 ND1 A:HIS391 2.6 0.4 1.0 SG A:CYS388 2.8 0.0 1.0 SG A:CYS364 2.9 0.3 1.0 CB A:CYS364 3.2 90.2 1.0 CE1 A:HIS359 3.4 98.2 1.0 CG A:HIS391 3.4 0.2 1.0 CB A:HIS391 3.5 0.2 1.0 CD2 A:HIS359 3.6 94.0 1.0 CB A:CYS388 3.6 0.2 1.0 CE1 A:HIS391 3.7 0.7 1.0 CA A:CYS364 3.8 88.5 1.0 ND1 A:HIS359 4.5 96.4 1.0 O A:CYS364 4.6 93.4 1.0 CG A:HIS359 4.6 92.7 1.0 N A:HIS391 4.6 0.8 1.0 C A:CYS364 4.6 89.0 1.0 CD2 A:HIS391 4.6 0.0 1.0 CA A:HIS391 4.7 0.5 1.0 NE2 A:HIS391 4.7 0.2 1.0 CD1 A:LEU379 4.8 67.4 1.0 N A:CYS364 4.9 83.6 1.0

Zinc binding site 2 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn602 b:92.4 occ:1.00 ND1 B:HIS391 2.6 96.5 1.0 NE2 B:HIS359 2.6 0.0 1.0 SG B:CYS388 2.8 0.5 1.0 SG B:CYS364 2.9 0.9 1.0 CB B:HIS391 3.2 1.0 1.0 CG B:HIS391 3.3 0.9 1.0 CE1 B:HIS359 3.5 0.6 1.0 CB B:CYS388 3.5 0.8 1.0 CB B:CYS364 3.6 97.1 1.0 CD2 B:HIS359 3.7 97.0 1.0 CE1 B:HIS391 3.7 98.2 1.0 CA B:CYS364 4.1 93.9 1.0 N B:HIS391 4.4 0.5 1.0 CA B:HIS391 4.4 0.1 1.0 CD2 B:HIS391 4.5 0.3 1.0 CD1 B:LEU379 4.6 76.7 1.0 ND1 B:HIS359 4.6 99.5 1.0 NE2 B:HIS391 4.7 0.8 1.0 CG B:HIS359 4.8 96.7 1.0 O B:CYS364 4.8 94.7 1.0 C B:CYS364 4.8 92.5 1.0 CA B:CYS388 5.0 0.3 1.0

Zinc binding site 3 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn606 b:87.1 occ:1.00 NE2 C:HIS359 2.6 0.1 1.0 ND1 C:HIS391 2.6 92.8 1.0 SG C:CYS388 2.7 87.6 1.0 SG C:CYS364 2.9 89.5 1.0 CG C:HIS391 3.0 94.5 1.0 CB C:HIS391 3.1 95.6 1.0 CB C:CYS364 3.3 89.7 1.0 CD2 C:HIS359 3.5 98.9 1.0 CE1 C:HIS359 3.5 1.0 1.0 CE1 C:HIS391 3.6 92.9 1.0 CA C:CYS364 3.7 86.8 1.0 CB C:CYS388 3.8 0.9 1.0 CD2 C:HIS391 4.1 95.3 1.0 NE2 C:HIS391 4.3 94.2 1.0 CD1 C:LEU379 4.3 68.0 1.0 CA C:HIS391 4.4 99.9 1.0 N C:HIS391 4.4 0.2 1.0 O C:CYS364 4.5 86.0 1.0 C C:CYS364 4.5 82.7 1.0 ND1 C:HIS359 4.6 99.7 1.0 CG C:HIS359 4.7 97.1 1.0 N C:CYS364 4.8 84.7 1.0

Zinc binding site 4 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn602 b:81.9 occ:1.00 NE2 D:HIS359 2.6 92.9 1.0 ND1 D:HIS391 2.6 88.0 1.0 SG D:CYS388 2.8 0.8 1.0 SG D:CYS364 2.9 0.5 1.0 CG D:HIS391 3.2 90.7 1.0 CB D:CYS364 3.2 79.8 1.0 CE1 D:HIS359 3.3 93.5 1.0 CB D:HIS391 3.4 92.9 1.0 CB D:CYS388 3.5 0.7 1.0 CE1 D:HIS391 3.5 87.7 1.0 CD2 D:HIS359 3.6 93.1 1.0 CA D:CYS364 3.8 79.3 1.0 CD2 D:HIS391 4.2 91.2 1.0 NE2 D:HIS391 4.3 89.7 1.0 ND1 D:HIS359 4.4 93.3 1.0 O D:CYS364 4.6 82.9 1.0 CG D:HIS359 4.6 92.4 1.0 N D:HIS391 4.6 1.0 1.0 CA D:HIS391 4.6 97.5 1.0 C D:CYS364 4.6 77.3 1.0 CD1 D:LEU379 4.8 64.1 1.0 CA D:CYS388 5.0 0.5 1.0 N D:CYS364 5.0 77.4 1.0

C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang. Distinct Gating Mechanisms Revealed By the Structures of A Multi-Ligand Gated K(+) Channel. Elife V. 1 00184 2012.
PubMed: 23240087
DOI: 10.7554/ELIFE.00184