Zinc in PDB 4eyd: Human Insulin

The structure of Human Insulin, PDB code: 4eyd was solved by M.P.Favero-Retto, L.C.Palmieri, L.M.T.R.Lima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.41 / 1.47
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	80.830, 80.830, 33.160, 90.00, 90.00, 120.00
R / Rfree (%)	15.3 / 22.3

The structure of Human Insulin also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Human Insulin (pdb code 4eyd). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Insulin, PDB code: 4eyd:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn101 b:15.7 occ:0.25 NE2 B:HIS10 2.0 17.6 1.0 CL B:CL102 2.7 35.8 0.2 CE1 B:HIS10 2.8 17.8 1.0 CD2 B:HIS10 3.1 16.7 1.0 ND1 B:HIS10 4.0 20.5 1.0 CG B:HIS10 4.1 18.1 1.0 O B:HOH206 4.9 28.0 1.0

Zinc binding site 2 out of 2 in the Human Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Insulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn101 b:17.9 occ:0.33 NE2 D:HIS10 2.1 15.4 1.0 CL D:CL102 2.6 64.2 0.3 CE1 D:HIS10 2.9 17.6 1.0 CD2 D:HIS10 3.1 15.3 1.0 O D:HOH228 4.0 32.5 1.0 O D:HOH207 4.0 40.3 1.0 ND1 D:HIS10 4.1 20.0 1.0 CG D:HIS10 4.2 16.9 1.0 O D:HOH204 4.6 23.7 1.0

M.P.Favero-Retto, L.C.Palmieri, T.A.Souza, F.C.Almeida, L.M.Lima. Structural Meta-Analysis of Regular Human Insulin in Pharmaceutical Formulations. Eur J Pharm Biopharm V. 85 1112 2013.
ISSN: ISSN 0939-6411
PubMed: 23692694
DOI: 10.1016/J.EJPB.2013.05.005