Zinc in PDB 4a4c: Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex

All present enzymatic activity of Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex:
2.7.10.2; 6.3.2.19;

The structure of Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex, PDB code: 4a4c was solved by H.Dou, L.Buetow, A.Hock, G.J.Sibbet, K.H.Vousden, D.T.Huang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.33 / 2.70
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	75.285, 75.285, 459.660, 90.00, 90.00, 120.00
R / Rfree (%)	20.3 / 26.8

The structure of Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Zinc atom in the Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex (pdb code 4a4c). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex, PDB code: 4a4c:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1436 b:92.5 occ:1.00 SG A:CYS381 2.2 78.0 1.0 SG A:CYS404 2.3 80.1 1.0 SG A:CYS401 2.4 90.5 1.0 SG A:CYS384 2.5 1.0 1.0 CB A:CYS381 2.9 77.0 1.0 CB A:CYS384 3.2 90.5 1.0 CB A:CYS404 3.3 59.7 1.0 N A:CYS384 3.5 79.6 1.0 CB A:CYS401 3.7 98.6 1.0 CA A:CYS384 3.9 88.0 1.0 N A:CYS401 4.2 80.9 1.0 CA A:CYS381 4.3 89.8 1.0 CB A:ILE383 4.4 70.4 1.0 N A:CYS404 4.4 80.0 1.0 CA A:CYS404 4.4 60.2 1.0 CA A:CYS401 4.4 90.8 1.0 C A:ILE383 4.5 79.7 1.0 O A:CYS401 4.6 89.5 1.0 C A:CYS381 4.7 90.9 1.0 OD1 A:ASN387 4.7 0.1 1.0 O A:CYS381 4.7 95.7 1.0 CA A:ILE383 4.8 76.4 1.0 N A:ILE383 4.8 82.8 1.0 CB A:ASN387 4.8 0.5 1.0 C A:CYS384 4.9 86.2 1.0 C A:CYS401 4.9 97.2 1.0 O A:ASN387 5.0 0.8 1.0 N A:ASN387 5.0 98.0 1.0

Zinc binding site 2 out of 2 in the Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of PHOSPHOTYR371-C-Cbl-UBCH5B-Zap-70 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1437 b:83.8 occ:1.00 ND1 A:HIS398 2.1 0.5 1.0 SG A:CYS416 2.4 68.2 1.0 SG A:CYS396 2.4 92.6 1.0 SG A:CYS419 2.4 71.5 1.0 CE1 A:HIS398 2.8 0.0 1.0 CB A:CYS396 2.9 73.5 1.0 CG A:HIS398 3.1 0.5 1.0 CB A:CYS416 3.2 57.5 1.0 CB A:CYS419 3.4 85.1 1.0 CB A:HIS398 3.6 85.6 1.0 N A:CYS419 3.7 95.2 1.0 NE2 A:HIS398 4.0 0.7 1.0 CD2 A:HIS398 4.1 0.6 1.0 CA A:CYS419 4.2 86.4 1.0 CB A:PHE418 4.2 83.0 1.0 CA A:CYS396 4.4 78.0 1.0 N A:HIS398 4.6 86.2 1.0 CA A:CYS416 4.6 66.1 1.0 CA A:HIS398 4.7 90.1 1.0 C A:PHE418 4.8 90.4 1.0 C A:CYS396 4.8 86.0 1.0 N A:ARG420 4.9 86.9 1.0 C A:CYS419 4.9 84.7 1.0 CA A:PHE418 4.9 81.4 1.0 N A:PHE418 5.0 79.8 1.0

H.Dou, L.Buetow, A.Hock, G.J.Sibbet, K.H.Vousden, D.T.Huang. Structural Basis For Autoinhibition and Phosphorylation-Dependent Activation of C-Cbl Nat.Struct.Mol.Biol. V. 19 184 2012.
ISSN: ISSN 1545-9993
PubMed: 22266821
DOI: 10.1038/NSMB.2231