Zinc in PDB 4a47: Crosstalk Between Cu(I) and Zn(II) Homeostasis

The structure of Crosstalk Between Cu(I) and Zn(II) Homeostasis, PDB code: 4a47 was solved by A.Badarau, A.Basle, S.J.Firbank, C.Denninson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	68.98 / 1.90
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	85.620, 85.620, 188.142, 90.00, 90.00, 120.00
R / Rfree (%)	19.466 / 24.302

The binding sites of Zinc atom in the Crosstalk Between Cu(I) and Zn(II) Homeostasis (pdb code 4a47). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crosstalk Between Cu(I) and Zn(II) Homeostasis, PDB code: 4a47:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crosstalk Between Cu(I) and Zn(II) Homeostasis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crosstalk Between Cu(I) and Zn(II) Homeostasis within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1065 b:22.2 occ:0.50 SG A:CYS12 2.3 23.0 1.0 SG A:CYS15 2.3 23.4 1.0 CB A:CYS12 3.3 22.5 1.0 N A:CYS15 3.4 22.6 1.0 CB A:CYS15 3.5 22.7 1.0 N A:CYS12 3.7 22.5 1.0 CA A:CYS15 3.9 22.7 1.0 CA A:CYS12 4.0 22.4 1.0 C A:SER14 4.4 22.5 1.0 C A:CYS12 4.5 21.4 1.0 O A:CYS12 4.5 20.4 1.0 CB A:SER14 4.6 23.4 1.0 C A:ASP11 4.7 23.4 1.0 N A:ASP11 4.8 23.7 1.0 N A:SER14 4.8 21.8 1.0 CA A:SER14 4.8 22.5 1.0

Zinc binding site 2 out of 4 in the Crosstalk Between Cu(I) and Zn(II) Homeostasis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crosstalk Between Cu(I) and Zn(II) Homeostasis within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1065 b:24.3 occ:0.50 SG B:CYS12 2.3 24.6 1.0 SG B:CYS15 2.4 23.4 1.0 CB B:CYS12 3.3 23.8 1.0 CB B:CYS15 3.5 23.5 1.0 N B:CYS15 3.5 23.2 1.0 N B:CYS12 3.7 24.5 1.0 CA B:CYS15 3.9 23.0 1.0 CA B:CYS12 4.0 24.5 1.0 C B:SER14 4.4 23.5 1.0 O B:CYS12 4.4 24.4 1.0 C B:CYS12 4.4 24.4 1.0 CB B:SER14 4.5 23.9 1.0 C B:ASP11 4.6 25.4 1.0 O B:HOH2007 4.7 20.1 0.5 N B:ASP11 4.8 25.2 1.0 CA B:SER14 4.8 23.9 1.0 N B:SER14 4.8 23.9 1.0

Zinc binding site 3 out of 4 in the Crosstalk Between Cu(I) and Zn(II) Homeostasis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crosstalk Between Cu(I) and Zn(II) Homeostasis within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1065 b:26.1 occ:0.50 SG C:CYS12 2.3 26.5 1.0 SG C:CYS15 2.4 25.1 1.0 CB C:CYS12 3.3 24.6 1.0 N C:CYS15 3.5 25.1 1.0 CB C:CYS15 3.6 25.3 1.0 N C:CYS12 3.8 24.9 1.0 CA C:CYS15 3.9 25.9 1.0 CA C:CYS12 4.0 24.8 1.0 C C:SER14 4.4 25.3 1.0 C C:CYS12 4.5 24.4 1.0 O C:CYS12 4.5 24.1 1.0 CB C:SER14 4.5 25.4 1.0 C C:ASP11 4.7 25.5 1.0 N C:ASP11 4.8 26.3 1.0 CA C:SER14 4.8 25.1 1.0 N C:SER14 4.8 24.4 1.0

Zinc binding site 4 out of 4 in the Crosstalk Between Cu(I) and Zn(II) Homeostasis


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crosstalk Between Cu(I) and Zn(II) Homeostasis within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1065 b:28.4 occ:0.50 SG D:CYS15 2.3 27.9 1.0 SG D:CYS12 2.4 26.2 1.0 N D:CYS15 3.4 25.9 1.0 CB D:CYS12 3.4 25.1 1.0 CB D:CYS15 3.5 26.5 1.0 N D:CYS12 3.7 26.2 1.0 CA D:CYS15 3.8 26.8 1.0 CA D:CYS12 4.0 25.7 1.0 C D:SER14 4.3 26.3 1.0 O D:CYS12 4.5 26.0 1.0 C D:CYS12 4.5 25.6 1.0 CB D:SER14 4.5 26.4 1.0 C D:ASP11 4.7 26.9 1.0 CA D:SER14 4.8 26.2 1.0 N D:ASP11 4.8 28.7 1.0 N D:SER14 4.8 25.9 1.0

A.Badarau, A.Basle, S.J.Firbank, C.Dennison. Investigating the Role of Zinc and Copper Binding Motifs of Trafficking Sites in the Cyanobacterium Synechocystis Pcc 6803. Biochemistry V. 52 6816 2013.
ISSN: ISSN 0006-2960
PubMed: 24050657
DOI: 10.1021/BI400492T