Zinc in PDB 3t9k: Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide

The structure of Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide, PDB code: 3t9k was solved by F.Sun, X.Pang, K.Zhang, J.Ma, Q.Zhou, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.30
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	108.258, 164.871, 41.665, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 22.5

The binding sites of Zinc atom in the Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide (pdb code 3t9k). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide, PDB code: 3t9k:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn999 b:37.7 occ:1.00 SG A:CYS440 2.3 37.4 1.0 SG A:CYS423 2.3 43.6 1.0 SG A:CYS420 2.3 38.2 1.0 SG A:CYS443 2.4 39.3 1.0 CB A:CYS443 3.1 33.5 1.0 CB A:CYS420 3.2 37.4 1.0 CB A:CYS423 3.4 42.4 1.0 CB A:CYS440 3.7 38.0 1.0 N A:CYS423 3.7 39.2 1.0 N A:CYS443 4.1 35.4 1.0 CA A:CYS423 4.1 42.4 1.0 N A:CYS440 4.1 33.2 1.0 CA A:CYS443 4.2 34.0 1.0 CB A:GLU425 4.4 44.5 1.0 CA A:CYS440 4.5 35.8 1.0 O A:HOH78 4.5 41.0 1.0 C A:ASP422 4.6 37.8 1.0 NZ A:LYS506 4.6 42.0 1.0 CB A:ASP422 4.6 34.3 1.0 CA A:CYS420 4.6 37.6 1.0 C A:CYS423 4.7 44.6 1.0 N A:ASP422 4.8 34.2 1.0 CD A:LYS506 4.8 40.2 1.0 CA A:ASP422 4.9 35.5 1.0 N A:ARG424 4.9 42.7 1.0 N A:GLU425 4.9 44.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of ACAP1 C-Portion Mutant S554D Fused with Integrin BETA1 Peptide within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn999 b:32.4 occ:1.00 SG B:CYS443 2.3 36.4 1.0 SG B:CYS423 2.3 35.5 1.0 SG B:CYS420 2.3 31.4 1.0 SG B:CYS440 2.3 33.7 1.0 CB B:CYS443 3.1 33.4 1.0 CB B:CYS420 3.2 29.2 1.0 CB B:CYS423 3.2 36.5 1.0 CB B:CYS440 3.6 31.9 1.0 N B:CYS423 3.7 34.4 1.0 N B:CYS440 4.0 28.8 1.0 CA B:CYS423 4.0 35.8 1.0 N B:CYS443 4.1 36.9 1.0 CA B:CYS443 4.2 35.8 1.0 O B:HOH12 4.3 40.0 1.0 CA B:CYS440 4.3 30.8 1.0 CB B:GLU425 4.4 31.4 1.0 NZ B:LYS506 4.4 39.3 1.0 C B:ASP422 4.6 36.0 1.0 C B:CYS423 4.6 35.0 1.0 CA B:CYS420 4.7 28.8 1.0 CB B:ASP422 4.7 35.7 1.0 N B:ARG424 4.8 32.7 1.0 N B:GLU425 4.8 30.8 1.0 N B:ASP422 4.9 32.3 1.0 CD B:LYS506 4.9 42.9 1.0 CA B:ASP422 4.9 34.6 1.0 CE B:LYS506 5.0 41.7 1.0 C B:CYS440 5.0 32.5 1.0 O B:CYS440 5.0 32.9 1.0

M.Bai, X.Pang, J.Lou, Q.Zhou, K.Zhang, J.Ma, J.Li, F.Sun, V.W.Hsu. Mechanistic Insights Into Regulated Cargo Binding By ACAP1 Protein J.Biol.Chem. V. 287 28675 2012.
ISSN: ISSN 0021-9258
PubMed: 22645133
DOI: 10.1074/JBC.M112.378810