Zinc in PDB 3f5l: Semi-Active E176Q Mutant of Rice BGLU1, A Plant Exoglucanase/Beta- Glucosidase

All present enzymatic activity of Semi-Active E176Q Mutant of Rice BGLU1, A Plant Exoglucanase/Beta- Glucosidase:
3.2.1.21;

The structure of Semi-Active E176Q Mutant of Rice BGLU1, A Plant Exoglucanase/Beta- Glucosidase, PDB code: 3f5l was solved by W.Chuenchor, J.R.Ketudat Cairns, S.Pengthaisong, R.C.Robinson, J.Yuvaniyama, C.-J.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.06 / 1.37
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	79.624, 100.804, 127.631, 90.00, 90.00, 90.00
R / Rfree (%)	18.1 / 19.8

The binding sites of Zinc atom in the Semi-Active E176Q Mutant of Rice BGLU1, A Plant Exoglucanase/Beta- Glucosidase (pdb code 3f5l). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Semi-Active E176Q Mutant of Rice BGLU1, A Plant Exoglucanase/Beta- Glucosidase, PDB code: 3f5l:

Zinc binding site 1 out of 1 in the Semi-Active E176Q Mutant of Rice BGLU1, A Plant Exoglucanase/Beta- Glucosidase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Semi-Active E176Q Mutant of Rice BGLU1, A Plant Exoglucanase/Beta- Glucosidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1001 b:6.2 occ:1.00 OD2 A:ASP65 2.0 5.8 1.0 OD2 B:ASP65 2.0 5.6 1.0 ND1 B:HIS68 2.0 7.7 1.0 ND1 A:HIS68 2.0 7.1 1.0 CG B:ASP65 2.7 6.9 1.0 CG A:ASP65 2.8 6.7 1.0 OD1 B:ASP65 2.8 6.7 1.0 OD1 A:ASP65 2.9 6.9 1.0 CE1 B:HIS68 3.0 7.2 1.0 CE1 A:HIS68 3.0 7.1 1.0 CG B:HIS68 3.0 6.5 1.0 CG A:HIS68 3.0 6.4 1.0 CB B:HIS68 3.4 7.5 1.0 CB A:HIS68 3.4 7.7 1.0 O A:HOH601 4.1 12.6 1.0 NE2 B:HIS68 4.1 7.2 1.0 NE2 A:HIS68 4.1 7.1 1.0 CD2 B:HIS68 4.2 7.5 1.0 O B:HOH579 4.2 11.7 1.0 CD2 A:HIS68 4.2 7.5 1.0 CB B:ASP65 4.2 5.8 1.0 CB A:ASP65 4.2 5.6 1.0 O B:HOH536 4.7 9.9 1.0 O A:HOH540 4.7 9.0 1.0 CA B:HIS68 4.7 7.6 1.0 CA A:HIS68 4.8 7.9 1.0 N B:HIS68 5.0 7.2 1.0

W.Chuenchor, S.Pengthaisong, R.C.Robinson, J.Yuvaniyama, J.Svasti, J.R.Ketudat Cairns. The Structural Basis of Oligosaccharide Binding By Rice BGLU1 Beta-Glucosidase J.Struct.Biol. V. 173 169 2011.
ISSN: ISSN 1047-8477
PubMed: 20884352
DOI: 10.1016/J.JSB.2010.09.021