Zinc in PDB 3egx: Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1

The structure of Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1, PDB code: 3egx was solved by J.Goldberg, J.D.Mancias, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 3.30
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	148.150, 97.230, 129.510, 90.00, 90.01, 90.00
R / Rfree (%)	20.6 / 29.2

The binding sites of Zinc atom in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1 (pdb code 3egx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1, PDB code: 3egx:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn765 b:34.5 occ:1.00 SG A:CYS85 2.4 36.9 1.0 SG A:CYS66 2.4 67.6 1.0 SG A:CYS88 2.6 70.8 1.0 CB A:CYS66 2.8 63.0 1.0 SG A:CYS61 2.8 58.6 1.0 CB A:CYS61 3.3 62.6 1.0 CB A:CYS85 3.3 39.1 1.0 CB A:CYS88 3.6 65.0 1.0 N A:CYS88 4.0 60.4 1.0 N A:ARG67 4.1 55.2 1.0 CA A:CYS66 4.2 61.5 1.0 CA A:CYS88 4.4 63.4 1.0 CB A:GLN90 4.4 73.5 1.0 N A:ALA68 4.4 53.3 1.0 CB A:ARG63 4.5 85.0 1.0 CB A:PHE87 4.5 58.5 1.0 C A:CYS66 4.6 55.9 1.0 C A:PHE87 4.7 59.9 1.0 CA A:CYS85 4.8 40.4 1.0 CB A:ALA68 4.8 49.4 1.0 CA A:CYS61 4.8 60.5 1.0 CA A:PHE87 5.0 58.2 1.0 CD A:ARG63 5.0 91.2 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Mammalian Copii-Coat Protein SEC23A/24A Complexed with the Snare Protein SEC22B and Bound to the Transport Signal Sequence of the Snare Protein BET1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1094 b:44.0 occ:1.00 SG B:CYS455 2.3 37.2 1.0 SG B:CYS452 2.3 60.5 1.0 SG B:CYS431 2.3 59.0 1.0 SG B:CYS434 2.5 47.6 1.0 CB B:CYS452 3.0 48.5 1.0 CB B:CYS431 3.1 57.3 1.0 CB B:CYS434 3.6 40.5 1.0 CB B:CYS455 3.7 38.9 1.0 N B:CYS434 3.7 41.5 1.0 N B:CYS455 3.9 38.7 1.0 OG1 B:THR436 4.2 30.1 1.0 CA B:CYS434 4.2 41.7 1.0 CA B:CYS455 4.2 38.6 1.0 CA B:CYS452 4.5 45.4 1.0 CA B:CYS431 4.5 56.7 1.0 CB B:SER433 4.5 47.3 1.0 CB B:THR436 4.6 27.8 1.0 C B:CYS455 4.6 39.7 1.0 C B:CYS434 4.7 40.2 1.0 N B:ARG435 4.7 40.4 1.0 C B:SER433 4.8 44.0 1.0 C B:LEU454 4.8 38.0 1.0 N B:THR436 4.8 30.4 1.0 N B:SER433 4.9 52.0 1.0 CB B:LEU454 4.9 37.1 1.0 C B:CYS431 5.0 58.6 1.0 N B:TYR456 5.0 41.9 1.0 CA B:SER433 5.0 47.2 1.0 CB B:ARG457 5.0 60.5 1.0 O B:CYS455 5.0 45.3 1.0

J.D.Mancias, J.Goldberg. Structural Basis of Cargo Membrane Protein Discrimination By the Human Copii Coat Machinery. Embo J. V. 27 2918 2008.
ISSN: ISSN 0261-4189
PubMed: 18843296
DOI: 10.1038/EMBOJ.2008.208