Zinc in PDB 3eb6: Structure of the CIAP2 Ring Domain Bound to UBCH5B

All present enzymatic activity of Structure of the CIAP2 Ring Domain Bound to UBCH5B:
6.3.2.19;

The structure of Structure of the CIAP2 Ring Domain Bound to UBCH5B, PDB code: 3eb6 was solved by P.D.Mace, K.Linke, F.-R.Schumacher, C.A.Smith, C.L.Day, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.52 / 3.40
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	137.226, 137.226, 111.870, 90.00, 90.00, 120.00
R / Rfree (%)	28.1 / 32

The binding sites of Zinc atom in the Structure of the CIAP2 Ring Domain Bound to UBCH5B (pdb code 3eb6). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of the CIAP2 Ring Domain Bound to UBCH5B, PDB code: 3eb6:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of the CIAP2 Ring Domain Bound to UBCH5B


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of the CIAP2 Ring Domain Bound to UBCH5B within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1001 b:82.9 occ:1.00 SG A:CYS581 2.3 83.6 1.0 SG A:CYS560 2.3 83.8 1.0 SG A:CYS578 2.3 85.9 1.0 SG A:CYS557 2.3 85.8 1.0 CB A:CYS581 3.2 86.6 1.0 CB A:CYS560 3.2 84.1 1.0 CB A:CYS557 3.3 85.7 1.0 CB A:CYS578 3.4 87.1 1.0 N A:CYS578 3.7 87.2 1.0 N A:CYS581 3.8 87.1 1.0 N A:CYS560 3.9 84.5 1.0 CA A:CYS578 4.0 87.2 1.0 CA A:CYS581 4.1 86.9 1.0 CA A:CYS560 4.1 84.1 1.0 O A:CYS578 4.2 87.3 1.0 C A:CYS578 4.4 87.4 1.0 CB A:LYS563 4.6 83.2 1.0 CA A:CYS557 4.8 85.7 1.0 O A:LYS563 4.8 83.1 1.0 CG2 A:VAL559 4.9 85.2 1.0 C A:ASP580 4.9 87.2 1.0 C A:VAL577 4.9 87.2 1.0 C A:CYS560 5.0 83.7 1.0

Zinc binding site 2 out of 2 in the Structure of the CIAP2 Ring Domain Bound to UBCH5B


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of the CIAP2 Ring Domain Bound to UBCH5B within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1002 b:82.3 occ:1.00 ND1 A:HIS574 1.9 82.5 1.0 CE1 A:HIS574 2.2 82.5 1.0 SG A:CYS572 2.3 81.0 1.0 SG A:CYS588 2.3 90.4 1.0 SG A:CYS591 2.3 88.2 1.0 CB A:CYS588 3.2 90.9 1.0 CB A:CYS572 3.2 82.4 1.0 CG A:HIS574 3.2 83.5 1.0 O A:CYS591 3.4 89.8 1.0 CB A:CYS591 3.5 88.9 1.0 NE2 A:HIS574 3.5 82.4 1.0 N A:CYS591 3.5 89.5 1.0 CA A:CYS591 3.9 89.2 1.0 CD2 A:HIS574 4.0 82.6 1.0 CB A:HIS574 4.1 84.0 1.0 C A:CYS591 4.1 89.4 1.0 CB A:SER593 4.4 88.2 1.0 CG2 A:ILE590 4.5 90.3 1.0 CA A:CYS572 4.5 82.4 1.0 CA A:CYS588 4.6 91.0 1.0 CB A:ILE590 4.6 89.8 1.0 O A:CYS572 4.6 82.8 1.0 C A:ILE590 4.6 89.6 1.0 C A:CYS572 4.7 82.7 1.0 O A:CYS588 4.9 90.8 1.0 C A:CYS588 4.9 90.8 1.0 N A:ILE590 4.9 90.0 1.0 CA A:ILE590 4.9 89.8 1.0 N A:SER593 5.0 88.5 1.0

P.D.Mace, K.Linke, R.Feltham, F.R.Schumacher, C.A.Smith, D.L.Vaux, J.Silke, C.L.Day. Structures of the CIAP2 Ring Domain Reveal Conformational Changes Associated with Ubiquitin-Conjugating Enzyme (E2) Recruitment. J.Biol.Chem. V. 283 31633 2008.
ISSN: ISSN 0021-9258
PubMed: 18784070
DOI: 10.1074/JBC.M804753200