Zinc in PDB 3e50: Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha

All present enzymatic activity of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha:
3.4.24.56;

The structure of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha, PDB code: 3e50 was solved by Q.Guo, M.Manolopoulou, W.-J.Tang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.30
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	262.172, 262.172, 90.522, 90.00, 90.00, 120.00
R / Rfree (%)	19.1 / 23.1

The binding sites of Zinc atom in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha (pdb code 3e50). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha, PDB code: 3e50:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1 b:2.0 occ:1.00 OE2 A:GLU189 0.9 2.0 1.0 NE2 A:HIS108 2.0 17.7 1.0 CD A:GLU189 2.1 19.3 1.0 O A:HOH1289 2.1 30.1 1.0 NE2 A:HIS112 2.2 24.4 1.0 OE1 A:GLU189 2.7 23.8 1.0 CD2 A:HIS108 2.9 22.9 1.0 CE1 A:HIS108 3.0 20.8 1.0 CD2 A:HIS112 3.1 24.4 1.0 CE1 A:HIS112 3.2 25.2 1.0 CG A:GLU189 3.5 25.0 1.0 NE2 A:GLN111 3.8 29.6 1.0 CG A:HIS108 4.1 22.1 1.0 ND1 A:HIS108 4.1 21.0 1.0 CG A:HIS112 4.3 21.7 1.0 ND1 A:HIS112 4.3 23.8 1.0 CD A:GLN111 4.5 29.4 1.0 OE1 A:GLN111 4.6 32.9 1.0 CE1 A:TYR831 4.9 25.2 1.0 CB A:GLU189 4.9 21.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Transforming Growth Factor-Alpha within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn51 b:2.0 occ:1.00 OE2 B:GLU189 1.0 18.1 1.0 O D:HIS12 1.6 63.9 1.0 NE2 B:HIS108 1.9 20.7 1.0 NE2 B:HIS112 2.1 22.6 1.0 CD B:GLU189 2.3 27.2 1.0 CE1 B:HIS108 2.8 28.2 1.0 C D:HIS12 2.9 66.6 1.0 OE1 B:GLU189 2.9 28.0 1.0 CD2 B:HIS108 2.9 22.2 1.0 CD2 B:HIS112 3.0 26.6 1.0 CE1 B:HIS112 3.1 28.8 1.0 N D:THR13 3.7 68.0 1.0 CG B:GLU189 3.9 25.8 1.0 CA D:THR13 3.9 69.2 1.0 ND1 B:HIS108 3.9 23.0 1.0 CA D:HIS12 3.9 66.9 1.0 N D:HIS12 4.0 67.0 1.0 CG B:HIS108 4.0 23.3 1.0 CG B:HIS112 4.2 25.3 1.0 ND1 B:HIS112 4.2 21.8 1.0 C D:SER11 4.3 66.9 1.0 NE2 B:GLN111 4.3 25.6 1.0 O D:SER11 4.4 65.7 1.0 CE1 B:TYR831 4.6 27.8 1.0 OE1 B:GLN111 4.7 28.1 1.0 N D:SER11 4.7 68.6 1.0 CB B:GLU189 4.8 28.9 1.0 CD B:GLN111 4.8 26.3 1.0 C D:THR13 4.9 70.1 1.0 CG2 D:THR13 4.9 68.2 1.0 OH B:TYR831 4.9 35.0 1.0

Q.Guo, M.Manolopoulou, Y.Bian, A.B.Schilling, W.J.Tang. Molecular Basis For the Recognition and Cleavages of Igf-II, Tgf-Alpha, and Amylin By Human Insulin-Degrading Enzyme. J.Mol.Biol. V. 395 430 2010.
ISSN: ISSN 0022-2836
PubMed: 19896952
DOI: 10.1016/J.JMB.2009.10.072