Zinc in PDB 2wc0: Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin

All present enzymatic activity of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin:
3.4.24.56;

The structure of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin, PDB code: 2wc0 was solved by M.Manolopoulou, Q.Guo, E.Malito, A.B.Schilling, W.J.Tang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.85 / 2.80
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	263.169, 263.169, 90.875, 90.00, 90.00, 120.00
R / Rfree (%)	17 / 22

The binding sites of Zinc atom in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin (pdb code 2wc0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin, PDB code: 2wc0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn3012 b:2.0 occ:1.00 OE1 A:GLU189 1.4 19.5 1.0 N D:PHE1 1.8 30.3 1.0 NE2 A:HIS108 1.8 24.9 1.0 NE2 A:HIS112 2.1 28.7 1.0 CD A:GLU189 2.5 30.4 1.0 CE1 A:HIS108 2.7 26.7 1.0 OE2 A:GLU189 2.8 33.7 1.0 CD2 A:HIS108 2.8 30.3 1.0 CA D:PHE1 3.0 31.1 1.0 CE1 A:HIS112 3.1 35.2 1.0 CD2 A:HIS112 3.1 33.2 1.0 C D:PHE1 3.7 30.9 1.0 ND1 A:HIS108 3.9 24.2 1.0 O D:HOH2001 3.9 20.6 1.0 CG A:HIS108 3.9 27.0 1.0 NE2 A:GLN111 4.0 28.3 1.0 CG A:GLU189 4.0 27.8 1.0 O D:PHE1 4.1 39.7 1.0 ND1 A:HIS112 4.2 32.8 1.0 CG A:HIS112 4.3 31.1 1.0 CB D:PHE1 4.3 30.7 1.0 N D:VAL2 4.4 32.0 1.0 OE1 A:GLN111 4.6 40.5 1.0 OH A:TYR831 4.7 35.3 1.0 CD A:GLN111 4.7 32.6 1.0 CE1 A:TYR831 4.9 32.1 1.0 CB A:GLU189 5.0 30.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Human Insulin Degrading Enzyme in Complex with Iodinated Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn3012 b:2.0 occ:1.00 OE2 B:GLU189 1.6 33.4 1.0 NE2 B:HIS112 1.9 31.8 1.0 NE2 B:HIS108 1.9 36.5 1.0 CD B:GLU189 2.4 39.6 1.0 OE1 B:GLU189 2.5 48.4 1.0 CA F:PHE1 2.6 50.0 1.0 CE1 B:HIS112 2.8 38.6 1.0 CE1 B:HIS108 2.9 38.0 1.0 CD2 B:HIS112 3.0 35.5 1.0 CD2 B:HIS108 3.0 39.5 1.0 CB F:PHE1 3.4 40.4 1.0 N F:PHE1 3.6 41.9 1.0 C F:PHE1 3.7 49.0 1.0 ND1 B:HIS112 3.9 32.5 1.0 N F:VAL2 4.0 45.6 1.0 ND1 B:HIS108 4.0 34.0 1.0 CG B:HIS112 4.0 34.8 1.0 CG B:GLU189 4.0 35.8 1.0 CG B:HIS108 4.1 33.2 1.0 NE2 B:GLN111 4.3 34.9 1.0 OH B:TYR831 4.3 43.0 1.0 CE1 B:TYR831 4.5 41.1 1.0 OE1 B:GLN111 4.6 34.6 1.0 O F:PHE1 4.6 41.0 1.0 CG F:PHE1 4.7 41.3 1.0 CD B:GLN111 4.8 35.4 1.0 CZ B:TYR831 4.8 42.2 1.0 CB B:GLU189 4.9 38.0 1.0

M.Manolopoulou, Q.Guo, E.Malito, A.B.Schilling, W.J.Tang. Molecular Basis of Catalytic Chamber-Assisted Unfolding and Cleavage of Human Insulin By Human Insulin Degrading Enzyme. J.Biol.Chem. V. 284 14177 2009.
ISSN: ISSN 0021-9258
PubMed: 19321446
DOI: 10.1074/JBC.M900068200