Zinc in PDB 2hpt: Crystal Structure of E. Coli Pepn (Aminopeptidase N)in Complex with Bestatin

All present enzymatic activity of Crystal Structure of E. Coli Pepn (Aminopeptidase N)in Complex with Bestatin:
3.4.11.2;

The structure of Crystal Structure of E. Coli Pepn (Aminopeptidase N)in Complex with Bestatin, PDB code: 2hpt was solved by A.Addlagatta, B.W.Matthews, L.Gay, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.94 / 2.30
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	120.670, 120.670, 170.840, 90.00, 90.00, 120.00
R / Rfree (%)	15.4 / 20.2

The binding sites of Zinc atom in the Crystal Structure of E. Coli Pepn (Aminopeptidase N)in Complex with Bestatin (pdb code 2hpt). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of E. Coli Pepn (Aminopeptidase N)in Complex with Bestatin, PDB code: 2hpt:

Zinc binding site 1 out of 1 in the Crystal Structure of E. Coli Pepn (Aminopeptidase N)in Complex with Bestatin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of E. Coli Pepn (Aminopeptidase N)in Complex with Bestatin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn950 b:21.4 occ:1.00 O2 A:BES960 1.9 14.5 1.0 OE1 A:GLU320 2.1 14.4 1.0 NE2 A:HIS297 2.1 11.0 1.0 NE2 A:HIS301 2.1 7.3 1.0 O3 A:BES960 2.5 22.4 1.0 CD A:GLU320 2.9 14.3 1.0 C2 A:BES960 2.9 17.1 1.0 C3 A:BES960 2.9 18.1 1.0 OE2 A:GLU320 3.0 14.3 1.0 CD2 A:HIS297 3.0 7.7 1.0 CD2 A:HIS301 3.1 4.3 1.0 CE1 A:HIS297 3.1 9.1 1.0 CE1 A:HIS301 3.1 5.8 1.0 C1 A:BES960 3.6 16.6 1.0 N2 A:BES960 3.8 10.1 1.0 OE1 A:GLU298 3.9 11.6 1.0 OH A:TYR381 4.0 12.8 1.0 N1 A:BES960 4.2 18.0 1.0 CE2 A:TYR381 4.2 11.6 1.0 CG A:HIS297 4.2 11.1 1.0 ND1 A:HIS297 4.2 10.7 1.0 ND1 A:HIS301 4.2 8.3 1.0 CG A:HIS301 4.2 8.2 1.0 CG A:GLU320 4.3 11.6 1.0 CZ A:TYR381 4.5 11.8 1.0 OE2 A:GLU298 4.5 14.2 1.0 OE1 A:GLU264 4.5 12.2 1.0 CD A:GLU298 4.6 13.2 1.0 CG2 A:THR323 4.7 8.9 1.0 OE2 A:GLU264 4.8 8.9 1.0 CA A:GLU320 4.8 10.8 1.0 CB A:GLU320 4.8 10.3 1.0 CD A:GLU264 4.9 11.7 1.0 C15 A:BES960 4.9 17.2 1.0 CB A:THR323 4.9 11.1 1.0

A.Addlagatta, L.Gay, B.W.Matthews. Structure of Aminopeptidase N From Escherichia Coli Suggests A Compartmentalized, Gated Active Site. Proc.Natl.Acad.Sci.Usa V. 103 13339 2006.
ISSN: ISSN 0027-8424
PubMed: 16938892
DOI: 10.1073/PNAS.0606167103