Zinc in PDB 2hih: Crystal Structure of Staphylococcus Hyicus Lipase

All present enzymatic activity of Crystal Structure of Staphylococcus Hyicus Lipase:
3.1.1.3;

The structure of Crystal Structure of Staphylococcus Hyicus Lipase, PDB code: 2hih was solved by J.J.W.Tiesinga, G.Van Pouderoyen, M.Nardini, B.W.Dijkstra, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.71 / 2.86
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	73.310, 77.960, 169.810, 90.00, 90.00, 90.00
R / Rfree (%)	20.8 / 26.3

The structure of Crystal Structure of Staphylococcus Hyicus Lipase also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of Staphylococcus Hyicus Lipase (pdb code 2hih). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Staphylococcus Hyicus Lipase, PDB code: 2hih:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Staphylococcus Hyicus Lipase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Staphylococcus Hyicus Lipase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:15.5 occ:1.00 OD2 A:ASP243 1.8 17.3 1.0 OE1 A:GLU72 2.0 17.6 1.0 NE2 A:HIS92 2.1 17.9 1.0 NE2 A:HIS98 2.3 18.1 1.0 OE2 A:GLU72 2.6 17.3 1.0 CD A:GLU72 2.6 17.2 1.0 CG A:ASP243 2.7 17.8 1.0 OD1 A:ASP243 3.0 17.6 1.0 CD2 A:HIS92 3.0 18.3 1.0 CD2 A:HIS98 3.1 18.4 1.0 CE1 A:HIS92 3.2 18.1 1.0 CE1 A:HIS98 3.3 18.2 1.0 OG A:SER69 4.1 16.3 1.0 CG A:GLU72 4.1 17.2 1.0 CB A:ASP243 4.1 18.1 1.0 CG A:HIS92 4.2 18.4 1.0 ND1 A:HIS92 4.2 18.1 1.0 CG A:HIS98 4.2 18.4 1.0 CD2 A:HIS71 4.3 17.3 1.0 ND1 A:HIS98 4.3 18.6 1.0 O A:HOH808 4.5 2.2 1.0 CD2 A:TYR96 4.6 18.1 1.0 O A:ASP243 4.8 17.6 1.0 CB A:TYR96 4.8 18.5 1.0 CD2 A:HIS215 4.9 18.8 1.0 NE2 A:HIS215 4.9 18.5 1.0 CB A:GLU72 4.9 17.3 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Staphylococcus Hyicus Lipase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Staphylococcus Hyicus Lipase within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1601 b:13.8 occ:1.00 OD2 B:ASP243 1.8 17.2 1.0 NE2 B:HIS92 2.0 18.6 1.0 OE1 B:GLU72 2.1 17.6 1.0 NE2 B:HIS98 2.3 18.6 1.0 OE2 B:GLU72 2.3 17.6 1.0 CD B:GLU72 2.5 17.2 1.0 CG B:ASP243 2.7 18.1 1.0 CE1 B:HIS92 2.9 18.4 1.0 OD1 B:ASP243 2.9 18.0 1.0 CD2 B:HIS92 3.1 18.7 1.0 CD2 B:HIS98 3.2 18.5 1.0 CE1 B:HIS98 3.3 18.6 1.0 CG B:GLU72 4.0 17.0 1.0 OG B:SER69 4.1 15.8 1.0 ND1 B:HIS92 4.1 18.3 1.0 CB B:ASP243 4.1 18.4 1.0 CG B:HIS92 4.2 18.6 1.0 CG B:HIS98 4.3 18.6 1.0 ND1 B:HIS98 4.3 18.7 1.0 CD2 B:HIS71 4.4 17.7 1.0 CD2 B:TYR96 4.7 18.3 1.0 CD2 B:HIS215 4.8 18.8 1.0 CB B:GLU72 4.9 17.2 1.0 O B:ASP243 4.9 18.2 1.0 CB B:TYR96 4.9 18.6 1.0 NE2 B:HIS215 4.9 18.4 1.0

J.J.Tiesinga, G.Van Pouderoyen, M.Nardini, S.Ransac, B.W.Dijkstra. Structural Basis of Phospholipase Activity of Staphylococcus Hyicus Lipase. J.Mol.Biol. V. 371 447 2007.
ISSN: ISSN 0022-2836
PubMed: 17582438
DOI: 10.1016/J.JMB.2007.05.041