Zinc in PDB 2hcn: Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus

All present enzymatic activity of Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus:
2.7.7.48;

The structure of Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus, PDB code: 2hcn was solved by M.P.Egloff, H.Malet, Marseilles Structural Genomics Program @ Afmb(Msgp), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.82 / 2.35
Space group	P 43
Cell size a, b, c (Å), α, β, γ (°)	110.064, 110.064, 68.572, 90.00, 90.00, 90.00
R / Rfree (%)	20.7 / 25.9

The structure of Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Zinc atom in the Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus (pdb code 2hcn). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus, PDB code: 2hcn:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2 b:65.0 occ:1.00 OE2 A:GLU440 2.2 55.3 1.0 SG A:CYS452 2.5 68.0 1.0 SG A:CYS449 2.5 66.1 1.0 ND1 A:HIS444 2.7 53.3 1.0 CE1 A:HIS444 2.7 52.9 1.0 CD A:GLU440 3.2 53.6 1.0 CG A:GLU440 3.4 53.5 1.0 CB A:CYS449 3.4 66.1 1.0 O A:THR451 3.9 68.7 1.0 CA A:CYS449 3.9 66.6 1.0 CB A:CYS452 3.9 68.2 1.0 NE2 A:HIS444 4.1 52.5 1.0 CG A:HIS444 4.1 56.4 1.0 OE1 A:GLU440 4.3 56.0 1.0 C A:THR451 4.4 68.3 1.0 N A:HIS450 4.4 67.5 1.0 CD1 A:LEU486 4.5 43.4 1.0 C A:CYS449 4.5 67.0 1.0 OG1 A:THR451 4.6 67.9 1.0 CD2 A:HIS444 4.7 55.0 1.0 N A:THR451 4.8 68.0 1.0 O A:GLU440 4.8 52.8 1.0 N A:CYS452 4.8 68.2 1.0 CB A:HIS444 4.9 57.3 1.0 O A:GLU448 4.9 66.2 1.0 CB A:GLU440 4.9 53.6 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Rna Dependent Rna Polymerase Domain From West Nile Virus within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn3 b:56.9 occ:1.00 NE2 A:HIS717 2.2 52.9 1.0 SG A:CYS852 2.2 61.2 1.0 SG A:CYS733 2.5 54.1 1.0 CE1 A:HIS717 3.1 48.8 1.0 CB A:CYS733 3.2 51.7 1.0 CD2 A:HIS717 3.2 48.2 1.0 CB A:CYS852 3.3 57.4 1.0 ND1 A:HIS717 4.3 51.2 1.0 C A:CYS733 4.3 50.8 1.0 CG A:HIS717 4.3 50.7 1.0 CA A:CYS733 4.3 51.1 1.0 N A:ARG734 4.4 50.3 1.0 NH1 A:ARG774 4.7 51.2 1.0 CA A:CYS852 4.7 57.5 1.0 O A:CYS733 4.7 50.8 1.0 O A:ARG734 4.8 49.8 1.0 CA A:ARG734 4.8 50.2 1.0 C A:ARG734 4.9 49.7 1.0

H.Malet, M.P.Egloff, B.Selisko, R.E.Butcher, P.J.Wright, M.Roberts, A.Gruez, G.Sulzenbacher, C.Vonrhein, G.Bricogne, J.M.Mackenzie, A.A.Khromykh, A.D.Davidson, B.Canard. Crystal Structure of the Rna Polymerase Domain of the West Nile Virus Non-Structural Protein 5 J.Biol.Chem. V. 282 10678 2007.
ISSN: ISSN 0021-9258
PubMed: 17287213
DOI: 10.1074/JBC.M607273200