Zinc in PDB 2hap: Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation

The structure of Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation, PDB code: 2hap was solved by D.A.King, L.Zhang, L.Guarente, R.Marmorstein, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.50
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	92.500, 92.500, 86.500, 90.00, 90.00, 90.00
R / Rfree (%)	24.8 / 30.2

The binding sites of Zinc atom in the Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation (pdb code 2hap). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation, PDB code: 2hap:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn136 b:22.6 occ:1.00 SG C:CYS81 2.3 21.4 1.0 SG C:CYS67 2.3 15.7 1.0 SG C:CYS64 2.4 20.9 1.0 SG C:CYS74 2.4 20.2 1.0 ZN C:ZN137 2.9 17.4 1.0 CB C:CYS81 3.1 20.8 1.0 CB C:CYS67 3.2 18.7 1.0 CB C:CYS74 3.3 18.2 1.0 CB C:CYS64 3.5 17.4 1.0 CA C:CYS74 3.9 19.2 1.0 N C:CYS64 4.0 19.8 1.0 OE1 C:GLN83 4.1 26.1 1.0 CB C:GLN83 4.2 21.4 1.0 CA C:CYS64 4.3 19.5 1.0 N C:CYS67 4.3 19.0 1.0 CA C:CYS67 4.3 18.8 1.0 SG C:CYS84 4.4 21.4 1.0 CA C:CYS81 4.5 22.3 1.0 CG C:GLN83 4.6 23.6 1.0 O C:CYS64 4.7 22.8 1.0 OH C:TYR95 4.7 13.4 1.0 SG C:CYS93 4.8 20.0 1.0 CD C:GLN83 4.8 26.1 1.0 C C:CYS64 4.8 21.3 1.0 N C:CYS84 4.9 22.5 1.0 N C:CYS74 4.9 21.0 1.0 C C:CYS74 4.9 20.0 1.0

Zinc binding site 2 out of 5 in the Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn137 b:17.4 occ:1.00 SG C:CYS64 2.3 20.9 1.0 SG C:CYS93 2.3 20.0 1.0 SG C:CYS84 2.3 21.4 1.0 SG C:CYS81 2.4 21.4 1.0 ZN C:ZN136 2.9 22.6 1.0 CB C:CYS64 3.1 17.4 1.0 CB C:CYS84 3.3 20.6 1.0 CB C:CYS93 3.3 20.1 1.0 CB C:CYS81 3.5 20.8 1.0 CA C:CYS93 4.0 18.1 1.0 N C:CYS81 4.0 23.2 1.0 CA C:CYS81 4.2 22.3 1.0 N C:CYS84 4.3 22.5 1.0 O C:CYS81 4.3 26.0 1.0 CA C:CYS84 4.3 21.6 1.0 CB C:ILE66 4.5 18.4 1.0 SG C:CYS67 4.5 15.7 1.0 CA C:CYS64 4.6 19.5 1.0 C C:CYS81 4.6 23.8 1.0 SG C:CYS74 4.7 20.2 1.0 OH C:TYR95 4.8 13.4 1.0 C C:CYS93 4.9 18.5 1.0 N C:HIS94 4.9 18.9 1.0 N C:CYS67 4.9 19.0 1.0

Zinc binding site 3 out of 5 in the Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn136 b:27.8 occ:0.50 ND1 D:HIS91 1.9 9.5 1.0 NE2 D:HIS80 2.2 17.4 1.0 CE1 D:HIS91 2.8 11.2 1.0 CG D:HIS91 2.9 8.8 1.0 CE1 D:HIS80 3.2 15.2 1.0 CD2 D:HIS80 3.2 15.2 1.0 CB D:HIS91 3.3 10.2 1.0 CA D:HIS91 3.7 13.1 1.0 NE2 D:HIS91 4.0 11.1 1.0 CD2 D:HIS91 4.0 8.6 1.0 ND1 D:HIS80 4.3 14.7 1.0 CG D:HIS80 4.3 15.6 1.0 O D:HIS91 4.6 13.8 1.0 N D:HIS91 4.6 15.7 1.0 C D:HIS91 4.7 13.9 1.0

Zinc binding site 4 out of 5 in the Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn137 b:22.3 occ:1.00 SG D:CYS81 2.3 19.0 1.0 SG D:CYS67 2.4 23.1 1.0 SG D:CYS74 2.4 21.3 1.0 SG D:CYS64 2.4 17.6 1.0 ZN D:ZN138 3.0 20.5 1.0 CB D:CYS81 3.2 19.8 1.0 CB D:CYS74 3.3 19.9 1.0 CB D:CYS64 3.5 17.1 1.0 CB D:CYS67 3.6 26.3 1.0 CA D:CYS74 3.9 18.4 1.0 N D:CYS64 4.0 16.8 1.0 CA D:CYS64 4.3 16.4 1.0 CB D:GLN83 4.3 24.2 1.0 SG D:CYS84 4.4 16.1 1.0 O D:CYS64 4.5 17.8 1.0 N D:CYS67 4.6 25.1 1.0 CA D:CYS67 4.6 25.9 1.0 CA D:CYS81 4.6 19.3 1.0 C D:CYS74 4.7 17.3 1.0 SG D:CYS93 4.7 19.5 1.0 N D:ASP75 4.8 17.7 1.0 C D:CYS64 4.8 17.1 1.0 CG D:GLN83 4.8 30.3 1.0 O D:ASP75 4.9 23.6 1.0 OD1 D:ASP75 4.9 22.0 1.0 OH D:TYR95 4.9 9.7 1.0

Zinc binding site 5 out of 5 in the Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Structure of A HAP1-18/Dna Complex Reveals That Protein/Dna Interactions Can Have Direct Allosteric Effects on Transcriptional Activation within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn138 b:20.5 occ:1.00 SG D:CYS93 2.3 19.5 1.0 SG D:CYS84 2.3 16.1 1.0 SG D:CYS64 2.3 17.6 1.0 SG D:CYS81 2.4 19.0 1.0 ZN D:ZN137 3.0 22.3 1.0 CB D:CYS64 3.1 17.1 1.0 CB D:CYS93 3.2 17.2 1.0 CB D:CYS84 3.3 18.4 1.0 CB D:CYS81 3.6 19.8 1.0 CA D:CYS93 4.0 15.5 1.0 N D:CYS81 4.1 18.5 1.0 N D:CYS84 4.3 21.1 1.0 CA D:CYS81 4.3 19.3 1.0 CA D:CYS84 4.3 19.6 1.0 O D:CYS81 4.4 22.4 1.0 CB D:ILE66 4.6 20.2 1.0 CA D:CYS64 4.6 16.4 1.0 SG D:CYS67 4.7 23.1 1.0 C D:CYS81 4.8 21.5 1.0 C D:CYS93 4.9 15.4 1.0 N D:CYS67 4.9 25.1 1.0 SG D:CYS74 4.9 21.3 1.0 OH D:TYR95 4.9 9.7 1.0 CB D:CYS67 4.9 26.3 1.0 CG2 D:ILE66 5.0 18.8 1.0

D.A.King, L.Zhang, L.Guarente, R.Marmorstein. Structure of HAP1-18-Dna Implicates Direct Allosteric Effect of Protein-Dna Interactions on Transcriptional Activation. Nat.Struct.Biol. V. 6 22 1999.
ISSN: ISSN 1072-8368
PubMed: 9886287
DOI: 10.1038/4893