Zinc in PDB 2ey4: Crystal Structure of A CBF5-NOP10-GAR1 Complex

The structure of Crystal Structure of A CBF5-NOP10-GAR1 Complex, PDB code: 2ey4 was solved by R.Rashid, B.Liang, H.Li, Southeast Collaboratory For Structural Genomics(Secsg), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.97 / 2.11
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	36.087, 75.361, 103.240, 95.90, 96.90, 94.99
R / Rfree (%)	21.1 / 25.5

The binding sites of Zinc atom in the Crystal Structure of A CBF5-NOP10-GAR1 Complex (pdb code 2ey4). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A CBF5-NOP10-GAR1 Complex, PDB code: 2ey4:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A CBF5-NOP10-GAR1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A CBF5-NOP10-GAR1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn201 b:89.6 occ:1.00 SG E:CYS20 2.4 60.1 1.0 SG E:CYS8 2.6 64.7 1.0 SG E:CYS11 2.7 52.5 1.0 SG E:CYS23 2.7 0.9 1.0 CB E:CYS8 2.9 58.4 1.0 O E:LYS10 3.1 61.5 1.0 CB E:CYS20 3.1 58.6 1.0 CB E:CYS23 3.9 98.2 1.0 N E:CYS23 3.9 79.0 1.0 CB E:CYS11 4.2 55.5 1.0 C E:LYS10 4.3 61.5 1.0 CB E:VAL22 4.3 74.4 1.0 CA E:CYS8 4.4 42.9 1.0 CB E:ARG13 4.4 86.1 1.0 CA E:CYS23 4.5 79.3 1.0 CA E:CYS20 4.5 57.0 1.0 N E:ARG13 4.8 50.0 1.0 C E:VAL22 4.8 80.3 1.0 CA E:VAL22 4.9 79.5 1.0 N E:VAL22 4.9 77.6 1.0 C E:CYS8 4.9 43.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A CBF5-NOP10-GAR1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A CBF5-NOP10-GAR1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn301 b:0.8 occ:1.00 SG F:CYS8 2.7 0.8 1.0 SG F:CYS11 2.7 82.7 1.0 SG F:CYS20 2.7 0.7 1.0 CB F:CYS11 3.0 81.5 1.0 CB F:CYS20 3.0 0.0 1.0 SG F:CYS23 3.0 0.4 1.0 CB F:CYS8 3.4 0.2 1.0 CB F:CYS23 3.4 0.4 1.0 OE2 F:GLU25 3.4 0.8 1.0 N F:CYS23 3.9 0.9 1.0 CD F:GLU25 4.2 0.5 1.0 CA F:CYS23 4.3 0.1 1.0 CA F:CYS11 4.5 78.6 1.0 CA F:CYS20 4.5 73.9 1.0 O F:PRO9 4.5 79.9 1.0 O F:CYS8 4.6 61.6 1.0 CA F:CYS8 4.6 61.2 1.0 CG F:GLU25 4.6 0.9 1.0 CB F:VAL22 4.7 0.0 1.0 C F:CYS8 4.7 62.8 1.0 CB F:ARG13 4.9 75.1 1.0 CB F:GLU25 4.9 0.6 1.0 C F:CYS23 5.0 0.1 1.0

R.Rashid, B.Liang, D.L.Baker, O.A.Youssef, Y.He, K.Phipps, R.M.Terns, M.P.Terns, H.Li. Crystal Structure of A CBF5-NOP10-GAR1 Complex and Implications in Rna-Guided Pseudouridylation and Dyskeratosis Congenita. Mol.Cell V. 21 249 2006.
ISSN: ISSN 1097-2765
PubMed: 16427014
DOI: 10.1016/J.MOLCEL.2005.11.017