Zinc in PDB 2ev6: Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc

The structure of Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc, PDB code: 2ev6 was solved by J.I.Kliegman, S.L.Griner, J.D.Helmann, R.G.Brennan, A.Glasfeld, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.14 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.710, 46.000, 74.390, 90.00, 93.17, 90.00
R / Rfree (%)	23.3 / 26

The binding sites of Zinc atom in the Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc (pdb code 2ev6). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc, PDB code: 2ev6:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1151 b:16.0 occ:1.00 ND1 A:HIS77 2.0 10.9 1.0 OE2 A:GLU102 2.1 14.7 1.0 OE2 A:GLU11 2.1 17.1 1.0 O A:HOH1249 2.2 39.1 1.0 OE1 A:GLU102 2.5 12.3 1.0 CD A:GLU102 2.6 12.5 1.0 OE2 A:GLU99 2.6 31.6 1.0 CD A:GLU11 2.8 18.3 1.0 CE1 A:HIS77 2.9 11.7 1.0 OE1 A:GLU11 2.9 15.5 1.0 CG A:HIS77 3.0 12.9 1.0 CB A:HIS77 3.5 11.4 1.0 CD A:GLU99 3.8 29.5 1.0 NE2 A:HIS77 4.0 12.7 1.0 CG A:GLU102 4.1 11.1 1.0 CD2 A:HIS77 4.1 10.7 1.0 O A:HOH1163 4.2 22.6 1.0 CG A:GLU11 4.3 14.5 1.0 O A:HOH1153 4.3 15.7 1.0 O A:HOH1194 4.3 29.6 1.0 O A:HOH1175 4.4 25.1 1.0 OE1 A:GLU99 4.5 32.4 1.0 CA A:HIS77 4.5 11.9 1.0 CG1 A:VAL98 4.8 11.7 1.0 CG A:GLU99 4.8 25.1 1.0 NE2 A:HIS103 4.9 17.5 1.0 CB A:GLU102 5.0 13.7 1.0 CA A:GLU99 5.0 17.6 1.0

Zinc binding site 2 out of 2 in the Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound to Zinc within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2151 b:15.9 occ:1.00 OE2 B:GLU11 1.9 15.8 1.0 ND1 B:HIS77 2.0 11.3 1.0 OE2 B:GLU102 2.0 16.4 1.0 O B:HOH2153 2.1 15.3 1.0 OE1 B:GLU102 2.6 13.1 1.0 CD B:GLU102 2.6 13.1 1.0 CD B:GLU11 2.8 19.0 1.0 CE1 B:HIS77 2.9 13.5 1.0 OE1 B:GLU11 3.0 16.0 1.0 OE1 B:GLU99 3.0 36.0 1.0 CG B:HIS77 3.1 13.8 1.0 CB B:HIS77 3.5 12.8 1.0 O B:HOH2188 4.0 19.7 1.0 CD B:GLU99 4.0 31.9 1.0 NE2 B:HIS77 4.1 13.8 1.0 CG B:GLU102 4.1 12.8 1.0 CD2 B:HIS77 4.2 11.3 1.0 O B:HOH2192 4.2 28.7 1.0 CG B:GLU11 4.2 17.3 1.0 O B:HOH2152 4.3 15.7 1.0 OE2 B:GLU99 4.4 36.9 1.0 O B:HOH2172 4.4 21.5 1.0 CA B:HIS77 4.5 12.2 1.0 CG1 B:VAL98 4.8 14.2 1.0 NE2 B:HIS103 4.8 17.8 1.0 CB B:GLU102 5.0 13.7 1.0

J.I.Kliegman, S.L.Griner, J.D.Helmann, R.G.Brennan, A.Glasfeld. Structural Basis For the Metal-Selective Activation of the Manganese Transport Regulator of Bacillus Subtilis. Biochemistry V. 45 3493 2006.
ISSN: ISSN 0006-2960
PubMed: 16533030
DOI: 10.1021/BI0524215