Zinc in PDB 1tlx: Thermolysin (Native)

All present enzymatic activity of Thermolysin (Native):
3.4.24.27;

The structure of Thermolysin (Native), PDB code: 1tlx was solved by A.C.English, S.H.Done, C.R.Groom, R.E.Hubbard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.10
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	93.910, 93.910, 131.170, 90.00, 90.00, 120.00
R / Rfree (%)	15.3 / 21.4

The structure of Thermolysin (Native) also contains other interesting chemical elements:

Calcium

(Ca)

4 atoms

The binding sites of Zinc atom in the Thermolysin (Native) (pdb code 1tlx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Thermolysin (Native), PDB code: 1tlx:

Zinc binding site 1 out of 1 in the Thermolysin (Native)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Thermolysin (Native) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:22.1 occ:1.00 NE2 A:HIS146 2.0 20.1 1.0 NE2 A:HIS142 2.0 17.3 1.0 O A:HOH1002 2.1 49.9 1.0 OE1 A:GLU166 2.2 24.9 1.0 OE2 A:GLU166 2.3 30.3 1.0 CD A:GLU166 2.8 23.3 1.0 O A:HOH1001 2.9 58.5 1.0 CE1 A:HIS146 2.9 17.1 1.0 CE1 A:HIS142 3.0 14.7 1.0 CD2 A:HIS146 3.0 16.2 1.0 CD2 A:HIS142 3.1 19.5 1.0 OH A:TYR157 3.7 22.2 0.4 OE2 A:GLU143 4.1 36.6 0.7 ND1 A:HIS146 4.1 18.6 1.0 ND1 A:HIS142 4.2 14.7 1.0 CG A:HIS146 4.2 17.9 1.0 CG A:HIS142 4.2 16.4 1.0 NE2 A:HIS231 4.3 25.5 1.0 CG A:GLU166 4.3 18.5 1.0 OE1 A:GLU143 4.4 24.5 0.7 CB A:SER169 4.4 16.0 1.0 OE1 A:GLU143 4.5 17.6 0.3 CA A:VAL317 4.5 33.4 1.0 O A:HOH1085 4.6 39.4 1.0 CD A:GLU143 4.6 27.0 0.7 CA A:GLU166 4.7 17.4 1.0 OG A:SER169 4.7 18.1 1.0 CZ A:TYR157 4.8 23.0 0.4 CD2 A:HIS231 4.8 30.7 1.0 CB A:GLU166 4.9 16.3 1.0 N A:VAL317 4.9 30.4 1.0 CE1 A:TYR157 4.9 25.0 0.4 C A:VAL317 5.0 32.6 1.0

A.C.English, S.H.Done, L.S.Caves, C.R.Groom, R.E.Hubbard. Locating Interaction Sites on Proteins: the Crystal Structure of Thermolysin Soaked in 2% to 100% Isopropanol. Proteins V. 37 628 1999.
ISSN: ISSN 0887-3585
PubMed: 10651278
DOI: 10.1002/(SICI)1097-0134(19991201)37:4<628::AID-PROT13>3.3.CO;2-7