Zinc in PDB 1nvg: N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form

All present enzymatic activity of N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form:
1.1.1.1;

The structure of N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form, PDB code: 1nvg was solved by L.Esposito, I.Bruno, F.Sica, C.A.Raia, A.Giordano, M.Rossi, L.Mazzarella, A.Zagari, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 2.50
Space group	I 41 2 2
Cell size a, b, c (Å), α, β, γ (°)	125.396, 125.396, 117.186, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 23.6

The binding sites of Zinc atom in the N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form (pdb code 1nvg). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form, PDB code: 1nvg:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn400 b:25.0 occ:1.00 OE2 A:GLU98 2.1 31.9 1.0 SG A:CYS104 2.2 22.3 1.0 SG A:CYS101 2.3 29.1 1.0 SG A:CYS112 2.4 26.6 1.0 CD A:GLU98 3.0 31.8 1.0 CB A:CYS112 3.2 29.3 1.0 CB A:CYS101 3.3 25.5 1.0 CB A:CYS104 3.4 23.5 1.0 CG A:GLU98 3.5 31.0 1.0 CA A:CYS112 3.5 27.6 1.0 N A:GLU98 3.7 32.3 1.0 N A:CYS101 3.8 26.0 1.0 N A:ASP113 3.8 31.7 1.0 N A:GLY99 3.9 33.8 1.0 OE1 A:GLU98 4.0 31.0 1.0 CA A:CYS101 4.1 25.1 1.0 N A:CYS104 4.1 22.3 1.0 C A:CYS112 4.2 29.2 1.0 CA A:CYS104 4.3 22.5 1.0 CA A:GLU98 4.5 32.2 1.0 CA A:GLY97 4.6 28.4 1.0 C A:GLY97 4.6 30.0 1.0 CB A:GLU98 4.6 31.3 1.0 N A:ASN100 4.6 30.4 1.0 C A:CYS101 4.7 24.8 1.0 C A:GLU98 4.7 34.3 1.0 CA A:GLY99 4.7 32.1 1.0 N A:SER114 4.7 28.8 1.0 O A:CYS101 4.8 22.0 1.0 N A:CYS112 4.8 28.4 1.0 C A:GLY99 4.8 32.1 1.0 CA A:ASP113 5.0 31.6 1.0 C A:ASN100 5.0 27.8 1.0

Zinc binding site 2 out of 2 in the N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of N249Y Mutant of the Alcohol Dehydrogenase From the Archaeon Sulfolobus Solfataricus-Tetragonal Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn500 b:35.6 occ:1.00 NE2 A:HIS68 2.1 32.1 1.0 OE2 A:GLU69 2.1 31.6 1.0 SG A:CYS154 2.3 32.4 1.0 SG A:CYS38 2.4 35.6 1.0 CD2 A:HIS68 3.0 30.2 1.0 CD A:GLU69 3.1 32.7 1.0 CE1 A:HIS68 3.1 32.0 1.0 CB A:CYS154 3.2 31.6 1.0 CB A:CYS38 3.3 35.6 1.0 CG A:GLU69 3.5 30.4 1.0 CG A:HIS68 4.2 31.3 1.0 ND1 A:HIS68 4.2 28.8 1.0 OE1 A:GLU69 4.2 31.2 1.0 OG A:SER40 4.2 40.9 1.0 CA A:CYS38 4.5 37.6 1.0 N A:CYS38 4.6 36.9 1.0 CA A:CYS154 4.7 31.5 1.0 CB A:SER40 4.7 39.6 1.0 NH1 A:ARG342 4.8 53.1 1.0 O A:HOH587 4.9 37.5 1.0 CB A:GLU69 5.0 28.1 1.0

L.Esposito, I.Bruno, F.Sica, C.A.Raia, A.Giordano, M.Rossi, L.Mazzarella, A.Zagari. Structural Study of A Single-Point Mutant of Sulfolobus Solfataricus Alcohol Dehydrogenase with Enhanced Activity Febs Lett. V. 539 14 2003.
ISSN: ISSN 0014-5793
PubMed: 12650918
DOI: 10.1016/S0014-5793(03)00173-X