Zinc in PDB 1n25: Crystal Structure of the SV40 Large T Antigen Helicase Domain

The structure of Crystal Structure of the SV40 Large T Antigen Helicase Domain, PDB code: 1n25 was solved by D.Li, R.Zhao, W.Lilyestrom, D.Gai, R.Zhang, J.A.Decaprio, E.Fanning, A.Jochimiak, G.Szakonyi, X.S.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.80
Space group	P 3 2 1
Cell size a, b, c (Å), α, β, γ (°)	120.321, 120.321, 132.202, 90.00, 90.00, 120.00
R / Rfree (%)	24.3 / 27.6

The binding sites of Zinc atom in the Crystal Structure of the SV40 Large T Antigen Helicase Domain (pdb code 1n25). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the SV40 Large T Antigen Helicase Domain, PDB code: 1n25:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the SV40 Large T Antigen Helicase Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the SV40 Large T Antigen Helicase Domain within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn700 b:56.1 occ:1.00 ND1 A:HIS317 1.9 34.7 1.0 SG A:CYS305 1.9 53.0 1.0 SG A:CYS302 2.1 59.0 1.0 NE2 A:HIS313 2.1 43.7 1.0 CG A:HIS317 2.7 35.4 1.0 CE1 A:HIS313 2.9 43.8 1.0 CE1 A:HIS317 2.9 34.3 1.0 CD2 A:HIS313 3.0 43.5 1.0 CB A:HIS317 3.0 38.3 1.0 CB A:CYS302 3.2 75.9 1.0 CB A:CYS305 3.7 52.9 1.0 CD2 A:HIS317 3.8 34.4 1.0 ND1 A:HIS313 3.9 43.9 1.0 NE2 A:HIS317 3.9 34.0 1.0 CG A:HIS313 4.0 43.6 1.0 N A:CYS305 4.1 59.5 1.0 CA A:CYS305 4.4 57.6 1.0 CB A:LYS304 4.5 68.0 1.0 CA A:HIS317 4.5 42.9 1.0 CA A:CYS302 4.6 81.7 1.0 O A:HIS313 4.8 42.2 1.0 C A:LYS304 4.8 59.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the SV40 Large T Antigen Helicase Domain


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the SV40 Large T Antigen Helicase Domain within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn700 b:53.7 occ:1.00 ND1 B:HIS317 2.0 37.0 1.0 SG B:CYS302 2.0 62.0 1.0 SG B:CYS305 2.1 60.6 1.0 NE2 B:HIS313 2.2 35.9 1.0 CE1 B:HIS317 2.8 34.4 1.0 CE1 B:HIS313 3.0 35.5 1.0 CG B:HIS317 3.1 38.6 1.0 CB B:CYS302 3.1 65.5 1.0 CD2 B:HIS313 3.3 37.1 1.0 CB B:HIS317 3.6 42.5 1.0 CB B:CYS305 3.8 62.0 1.0 NE2 B:HIS317 4.0 35.4 1.0 N B:CYS305 4.0 63.8 1.0 CD2 B:HIS317 4.1 36.4 1.0 ND1 B:HIS313 4.1 37.6 1.0 CG B:HIS313 4.3 37.9 1.0 CB B:LYS304 4.4 66.3 1.0 CA B:CYS302 4.5 70.1 1.0 CA B:CYS305 4.5 64.6 1.0 CB B:PHE299 4.9 81.0 1.0 C B:LYS304 4.9 64.2 1.0 CA B:PHE299 4.9 75.2 1.0 C B:CYS302 5.0 66.2 1.0

D.Li, R.Zhao, W.Lilyestrom, D.Gai, R.Zhang, J.A.Decaprio, E.Fanning, A.Jochimiak, G.Szakonyi, X.S.Chen. Structure of the Replicative Helicase of the Oncoprotein SV40 Large Tumour Antigen Nature V. 423 512 2003.
ISSN: ISSN 0028-0836
PubMed: 12774115
DOI: 10.1038/NATURE01691