Zinc in PDB 1my0: Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw)

The structure of Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw), PDB code: 1my0 was solved by R.Jin, E.Gouaux, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.95 / 1.90
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	114.477, 164.204, 47.403, 90.00, 90.00, 90.00
R / Rfree (%)	22.6 / 24.9

The binding sites of Zinc atom in the Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw) (pdb code 1my0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw), PDB code: 1my0:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:21.6 occ:1.00 OE1 A:GLU42 2.1 20.8 1.0 OE2 B:GLU166 2.1 23.7 1.0 NE2 A:HIS46 2.2 23.7 1.0 OE1 B:GLU166 2.7 24.2 1.0 CD B:GLU166 2.7 25.6 1.0 CD A:GLU42 3.0 23.0 1.0 CD2 A:HIS46 3.1 23.9 1.0 CE1 A:HIS46 3.2 25.7 1.0 O B:HOH866 3.3 12.6 1.0 OE2 A:GLU42 3.3 22.4 1.0 CG B:GLU166 4.2 22.2 1.0 N B:SER168 4.2 15.9 1.0 CD2 A:LEU241 4.3 21.8 1.0 CG A:HIS46 4.3 22.9 1.0 ND1 A:HIS46 4.3 23.5 1.0 CG A:GLU42 4.3 20.1 1.0 O B:ALA165 4.6 17.6 1.0 CB B:SER168 4.7 16.2 1.0 CB A:GLU42 4.7 16.9 1.0 CA B:SER168 4.8 15.8 1.0 CB B:GLU166 4.8 21.7 1.0 C B:PRO167 4.9 18.7 1.0 CA B:PRO167 5.0 18.4 1.0 CA B:GLU166 5.0 20.7 1.0

Zinc binding site 2 out of 5 in the Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn702 b:27.8 occ:1.00 NE2 B:HIS23 2.1 26.8 1.0 O B:HOH828 2.2 23.9 1.0 OE2 B:GLU30 2.2 24.8 1.0 OE1 B:GLU30 2.9 23.4 1.0 CD B:GLU30 2.9 22.8 1.0 CD2 B:HIS23 3.0 24.1 1.0 CE1 B:HIS23 3.2 25.2 1.0 CD B:LYS20 4.0 23.2 1.0 CG B:HIS23 4.2 26.6 1.0 NZ B:LYS20 4.2 24.2 1.0 ND1 B:HIS23 4.2 26.1 1.0 CG B:GLU30 4.3 24.7 1.0 O B:GLU30 4.4 22.6 1.0 CE B:LYS20 4.7 22.2 1.0 CD1 B:LEU26 4.8 42.8 1.0

Zinc binding site 3 out of 5 in the Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn704 b:24.1 occ:1.00 NE2 B:HIS46 2.0 18.8 1.0 OE1 B:GLU42 2.2 22.6 1.0 CD2 B:HIS46 3.0 19.2 1.0 CD B:GLU42 3.1 20.9 1.0 CE1 B:HIS46 3.1 21.7 1.0 OE2 B:GLU42 3.3 23.2 1.0 CD2 B:LEU241 3.7 19.5 1.0 CE B:LYS45 4.0 27.0 1.0 NZ B:LYS45 4.0 22.7 1.0 OE1 B:GLN244 4.0 38.9 1.0 CG B:HIS46 4.1 18.5 1.0 ND1 B:HIS46 4.2 21.5 1.0 CG B:GLU42 4.4 19.6 1.0 CB B:GLU42 4.7 15.4 1.0

Zinc binding site 4 out of 5 in the Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw) within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn703 b:35.7 occ:1.00 OD2 C:ASP65 2.3 27.0 1.0 OD1 C:ASP65 2.8 27.5 1.0 CG C:ASP65 2.8 26.9 1.0 CB C:ASP67 4.0 39.3 1.0 CB C:ASP65 4.3 25.6 1.0 CG C:ASP67 4.8 43.2 1.0 CG2 C:THR68 4.9 31.1 1.0 N C:ASP67 4.9 35.7 1.0

Zinc binding site 5 out of 5 in the Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Titration Experiments (Ampa Co-Crystals Soaked in 100 Nm Brw) within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn705 b:57.0 occ:1.00 OE1 C:GLU42 2.3 35.1 1.0 NE2 C:HIS46 2.4 45.1 1.0 CE1 C:HIS46 3.3 45.1 1.0 CD C:GLU42 3.4 35.4 1.0 CD2 C:HIS46 3.4 43.4 1.0 NZ C:LYS45 3.6 46.0 1.0 CE C:LYS45 3.7 43.7 1.0 OE2 C:GLU42 3.7 38.7 1.0 CD2 C:LEU241 4.3 27.8 1.0 ND1 C:HIS46 4.5 44.4 1.0 CG C:HIS46 4.5 42.8 1.0 CG C:GLU42 4.7 32.8 1.0 CB C:GLU42 5.0 28.1 1.0

R.Jin, E.Gouaux. Probing the Function, Conformational Plasticity, and Dimer-Dimer Contacts of the GLUR2 Ligand-Binding Core: Studies of 5-Substituted Willardiines and GLUR2 S1S2 in the Crystal Biochemistry V. 42 5201 2003.
ISSN: ISSN 0006-2960
PubMed: 12731861
DOI: 10.1021/BI020632T