Atomistry » Zinc » PDB 1jpu-1k51 » 1jqb
Atomistry »
  Zinc »
    PDB 1jpu-1k51 »
      1jqb »

Zinc in PDB 1jqb: Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability

Enzymatic activity of Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability

All present enzymatic activity of Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability:
1.1.1.2;

Protein crystallography data

The structure of Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability, PDB code: 1jqb was solved by I.Levin, F.Frolow, O.Bogin, M.Peretz, Y.Hacham, Y.Burstein, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.01 / 1.97
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 86.700, 147.800, 125.100, 90.00, 90.00, 90.00
R / Rfree (%) 21.9 / 24.7

Zinc Binding Sites:

The binding sites of Zinc atom in the Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability (pdb code 1jqb). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability, PDB code: 1jqb:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 1jqb

Go back to Zinc Binding Sites List in 1jqb
Zinc binding site 1 out of 4 in the Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1353

b:35.8
occ:1.00
 OD2 A:ASP1150 1.9 25.5 1.0
NE2 A:HIS1059 2.2 24.9 1.0
SG A:CYS1037 2.5 29.3 1.0
CG A:ASP1150 3.1 25.9 1.0
CD2 A:HIS1059 3.1 25.4 1.0
CE1 A:HIS1059 3.2 26.6 1.0
CB A:CYS1037 3.2 30.1 1.0
OD1 A:ASP1150 3.6 23.0 1.0
OG A:SER1039 3.7 36.4 1.0
OE2 A:GLU1060 4.0 23.7 1.0
CB A:SER1039 4.1 34.6 1.0
CB A:ASP1150 4.3 22.6 1.0
ND1 A:HIS1059 4.3 25.5 1.0
CG A:HIS1059 4.3 23.9 1.0
CE A:MET1151 4.4 25.3 1.0
CD A:GLU1060 4.4 23.5 1.0
CG A:GLU1060 4.7 23.2 1.0
CA A:CYS1037 4.7 29.5 1.0

Zinc binding site 2 out of 4 in 1jqb

Go back to Zinc Binding Sites List in 1jqb
Zinc binding site 2 out of 4 in the Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn2353

b:41.9
occ:1.00
 OD1 B:ASP2150 2.0 29.9 1.0
NE2 B:HIS2059 2.3 28.9 1.0
OE2 B:GLU2060 2.3 31.1 1.0
SG B:CYS2037 2.5 30.1 1.0
CB B:CYS2037 3.0 30.3 1.0
CG B:ASP2150 3.1 26.0 1.0
CD2 B:HIS2059 3.1 27.2 1.0
CD B:GLU2060 3.2 25.7 1.0
CE1 B:HIS2059 3.3 29.9 1.0
CB B:ASP2150 3.6 21.2 1.0
CG B:GLU2060 3.7 24.0 1.0
CE B:MET2151 3.8 17.5 1.0
O B:HOH241 3.8 36.4 1.0
OG B:SER2039 4.1 41.5 1.0
OD2 B:ASP2150 4.2 28.7 1.0
OE1 B:GLU2060 4.2 25.1 1.0
CG B:HIS2059 4.3 26.9 1.0
ND1 B:HIS2059 4.3 28.6 1.0
CA B:CYS2037 4.4 31.3 1.0
O B:HOH139 4.7 31.6 1.0
O B:HOH191 5.0 26.5 1.0

Zinc binding site 3 out of 4 in 1jqb

Go back to Zinc Binding Sites List in 1jqb
Zinc binding site 3 out of 4 in the Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn3353

b:42.0
occ:1.00
 OD2 C:ASP3150 2.2 34.4 1.0
NE2 C:HIS3059 2.2 35.2 1.0
SG C:CYS3037 2.6 37.8 1.0
CE1 C:HIS3059 3.1 34.4 1.0
CG C:ASP3150 3.1 30.2 1.0
CB C:CYS3037 3.3 37.9 1.0
CD2 C:HIS3059 3.3 34.5 1.0
OD1 C:ASP3150 3.5 26.8 1.0
CB C:SER3039 3.8 51.6 1.0
ND1 C:HIS3059 4.3 34.5 1.0
CB C:ASP3150 4.3 26.0 1.0
OE2 C:GLU3060 4.3 29.6 1.0
OG C:SER3039 4.4 55.1 1.0
CG C:HIS3059 4.4 33.0 1.0
CE C:MET3151 4.6 23.0 1.0
CD C:GLU3060 4.6 29.8 1.0
CA C:CYS3037 4.8 39.3 1.0
CG C:GLU3060 4.9 29.5 1.0

Zinc binding site 4 out of 4 in 1jqb

Go back to Zinc Binding Sites List in 1jqb
Zinc binding site 4 out of 4 in the Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Alcohol Dehydrogenase From Clostridium Beijerinckii: Crystal Structure of Mutant with Enhanced Thermal Stability within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn4353

b:36.2
occ:1.00
 OD2 D:ASP4150 1.9 27.0 1.0
NE2 D:HIS4059 2.2 21.9 1.0
SG D:CYS4037 2.5 27.6 1.0
O D:HOH112 2.6 36.6 1.0
CG D:ASP4150 3.1 25.7 1.0
CD2 D:HIS4059 3.1 21.3 1.0
CE1 D:HIS4059 3.2 22.1 1.0
CB D:CYS4037 3.3 28.1 1.0
OD1 D:ASP4150 3.7 28.1 1.0
OG D:SER4039 3.8 37.6 1.0
OE2 D:GLU4060 3.9 27.3 1.0
CB D:SER4039 4.0 36.0 1.0
CB D:ASP4150 4.2 22.9 1.0
ND1 D:HIS4059 4.3 20.4 1.0
CG D:HIS4059 4.3 18.8 1.0
CE D:MET4151 4.3 18.2 1.0
CD D:GLU4060 4.5 22.7 1.0
CA D:CYS4037 4.8 27.4 1.0
CG D:GLU4060 4.8 20.8 1.0

Reference:

O.Bogin, I.Levin, Y.Hacham, S.Tel-Or, M.Peretz, F.Frolow, Y.Burstein. Structural Basis For the Enhanced Thermal Stability of Alcohol Dehydrogenase Mutants From the Mesophilic Bacterium Clostridium Beijerinckii: Contribution of Salt Bridging Protein Sci. V. 11 2561 2002.
ISSN: ISSN 0961-8368
PubMed: 12381840
DOI: 10.1110/PS.0222102
Page generated: Sun Oct 13 03:49:27 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy